Labshake search
Citations for GE Life Sciences :
2801 - 2850 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS/PAGE gel and transferred to Protran nitrocellulose membrane (0.2 μm, GE Healthcare). Immunoblotting was performed by incubating with primary antibodies diluted in Tris-buffered saline (20mM Tris pH 7.6 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
-
bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
-
bioRxiv - Biochemistry 2023Quote: ... The dialyzed proteins were concentrated and purified by SEC (HiLoad Superdex-200 16/600 column, GE Healthcare) into tau buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... the protein was transferred to an Amersham™ Hybond™ P0.45 polyvinylidene difluoride (PVDF) membrane (GE Healthcare) in 1x Tris/Glycine transfer buffer (National diagnostics ...
-
bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...
-
bioRxiv - Neuroscience 2023Quote: ... the protein was cleaved with Tev protease and purified on a HiTrap Q HP column (GE Healthcare) using a salt gradient ...
-
bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... Acetylated LCas12a proteins were purified following the steps of Ni-NTA column (HisTap™ HP, GE Healthcare), ion exchange chromatography (HiTrap™ SP HP ...
-
bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was passed through a HiLoad® 26/600 Superdex® 75 pg (GE Healthcare) size exclusion chromatography column and the resulting pure protein was stored in gel filtration buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were concentrated and loaded onto a Superdex 200 column (10/300 GL; GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
-
bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were collected with 50 ul of protein A sepharose (17-0780-01; GE Healthcare) at 4°C for two hours with rotation ...
-
bioRxiv - Biophysics 2024Quote: ... Dialysed protein was further purified by ion-exchange chromatography using a HiTrap-FF Q column (GE Healthcare), resulting in the elution of N-myc proteins in the flow-through and at low salt concentrations ...
-
bioRxiv - Microbiology 2024Quote: ... Collected supernatant containing the Fc fused nanobody was incubated overnight with protein-A Sepharose beads (GE Healthcare), after which proteins were purified using poly-prep chromatography columns (Bio-Rad) ...
-
bioRxiv - Biochemistry 2024Quote: ... the protein was additionally purified by size-exclusion chromatography (Superdex 200 16/60 column; GE Healthcare™).
-
bioRxiv - Neuroscience 2024Quote: ... All proteins were further purified by SEC using a Superdex 200 10/300 GL column (GE Healthcare) with 20 mM Tis-HCl at pH 8.0 ...
-
bioRxiv - Biophysics 2024Quote: ... The cleaved proteins were further purified by cation exchange chromatography using HiTrap SP HP column (GE Healthcare) equilibrated in buffer C ...
-
bioRxiv - Microbiology 2024Quote: ... The mAbs were isolated from the culture supernatants by affinity chromatography using Protein G beads (GE Healthcare) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2024Quote: ... 25 mg of protein was incubated with 25 µl IgG-sepharose 6 FAST Flow beads (GE Healthcare) for 1 h on a rotator at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
-
bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
-
bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit or antirat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
-
bioRxiv - Cancer Biology 2019Quote: ... incubated with corresponding horseradish peroxidase-linked secondary antibodies (Amersham, GE Healthcare) for 1h at room temperature and detected by chemiluminescence imaging system (Bio-Rad).
-
bioRxiv - Cancer Biology 2021Quote: ... incubated with appropriate HRP-labelled secondary antibodies (GE Healthcare Life Sciences), washed as before and developed using ECL (Thermo Fisher ...
-
bioRxiv - Bioengineering 2020Quote: ... The antibody was detected using the ECL plus reagents (GE Healthcare). Detection was done in a dark room ...
-
bioRxiv - Systems Biology 2021Quote: ... primary antibodies and polyclonal goat anti-mouse HRP conjugated (GE Healthcare) secondary antibody ...
-
bioRxiv - Microbiology 2020Quote: ... Secondary HRP-conjugated antibodies used were donkey anti-rabbit (GE Healthcare) and goat anti-mouse (Sigma-Aldrich).
-
bioRxiv - Immunology 2021Quote: ... for IgG antibody or LambdaFabSelect Agarose Beads (GE Healthcare Life Sciences) for Fab at 4°C on a rotating shaker overnight ...
-
bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used was anti-rabbit HRP-conjugated from GE Healthcare.
-
bioRxiv - Cancer Biology 2020Quote: ... and HRP-conjugated secondary antibodies: anti-rabbit (GE Healthcare Cat# NA934); anti-mouse (GE Healthcare Cat# NA931 ...
-
bioRxiv - Biochemistry 2022Quote: ... HRP-linked monkey anti-rabbit secondary antibody was from GE Healthcare Life Sciences (Little Chalfont ...
-
bioRxiv - Cancer Biology 2022Quote: ... The secondary antibody was anti-rabbit IgG (GE Healthcare, 1:30000). Signal detection was performed with SuperSignal™ West Femto Maximum Sensitivity Substrate chemiluminescence detection system (ThermoFisher ...
-
bioRxiv - Microbiology 2022Quote: ... peroxidase-conjugated anti-mouse-IgG antibody (GE Healthcare, Chicago, IL, USA), diluted 1:5,000 in blocking buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... the HRP-linked anti-rabbit IgG secondary antibody (GE Healthcare, NA934) was used at a dilution of 1:5000 or 1:10000 ...
-
bioRxiv - Microbiology 2021Quote: ... and HRP-linked donkey anti-mouse IgG antibody (NA931V; GE Healthcare). Cell supernatants containing the pseudotype viral particles were centrifuged at 109,000 g or 35 min at 4 °C using a TLA100.3 rotor with an Optima TLX ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2022Quote: ... HRP-coupled antibodies were detected by enhanced chemiluminescence (Amersham, GE Healthcare) and X-ray films ...
-
bioRxiv - Developmental Biology 2019Quote: ... After incubation with HRP-conjugated secondary antibodies (GE Healthcare, 1:5000), signals were detected using SuperSignal™ Femto West Chemoluminescent Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-goat as the secondary antibody (GE Healthcare) were used at a dilution of 1:3000.
-
bioRxiv - Cancer Biology 2019Quote: ... anti-mouse or antirabbit HRP-conjugated antibodies (GE Healthcare, 1:5000).
-
bioRxiv - Microbiology 2020Quote: ... Bound antibody was visualized using ECL chemiluminescent detection reagent (GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... Phages were detected by HRP-conjugated anti-M13 antibody (GE Healthcare). For signal generation ...
-
bioRxiv - Biochemistry 2020Quote: ... Antibody complexes were detected using enhanced chemiluminescence detection reagents (GE Healthcare) and digital images captured using GeneSnap (SynGene ...
-
bioRxiv - Immunology 2020Quote: ... Purification of labelled antibodies was performed using NAP5 Columns (GE HealthCare). The dye to antibody ratio was quantified using Nanodrop and only antibodies with a composition of 3-4 Alexa Fluor 405 and 0.9-1.2 Alexa Fluor 647 per antibody were used for imaging.
-
bioRxiv - Immunology 2021Quote: ... Hit detected by fluorescent secondary antibody using ImageQuant software (GE Healthcare). An expression vector (plRES-hEGFR- IRES-ZsGreen1 ...