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Citations for GE Life Sciences :
2601 - 2650 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Eluted protein was concentrated and further purified on a Superose 6 10/300 Increase column (GE Healthcare) with 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were further purified using 16/600 Superdex 75 pg size-exclusion chromatography columns (GE Healthcare) equilibrated in protein buffer (20mM Tris pH 7.8 ...
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bioRxiv - Neuroscience 2020Quote: ... Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare) followed by incubation for 2 hr at 4°C ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The reconstituted protein was further purified on the Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in a buffer comprising 20 mM Bis-Tris pH 6.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions used were ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibody and dilution used was rat anti-HA-HRP 3F10 (Roche Applied Science) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions were ...
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bioRxiv - Cell Biology 2021Quote: ... Labeled proteins were purified by final buffer exchange using size exclusion chromatography (Superdex 200 column, GE Healthcare) in the appropriate buffer (i.e ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated DnaA protein was subjected to size exclusion chromatography on an Äkta pure instrument (GE Healthcare). 200 μL of concentrated DnaA-6xHis was applied to a Superdex 200 Increase 10/30 column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting reagents (Amersham, GE Healthcare, UK) or SuperSignal West Dura Extended Duration Substrate (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... denatured proteins were then separated via SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Amersham). Blots were preincubated in a blocking solution of 5% BSA in 0.2% TBST (0.1 M Tris base ...
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bioRxiv - Plant Biology 2020Quote: ... the purified RCA protein was immobilized on a carboxy-methylated dextran sensor chip (CM5) chip (GE Healthcare) using an amine coupling method kit (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 10 mM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Microbiology 2021Quote: ... recombinant protein was purified from the filtered culture supernatant using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL western blotting detection reagent (GE Healthcare). Thereafter ...
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bioRxiv - Microbiology 2019Quote: ... Typically 100 µg of protein samples were injected into Superdex 200 10/300 increase column (GE Healthcare) by a Shimadzu autosampler coupled HPLC machine ...
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bioRxiv - Cell Biology 2021Quote: ... between 600-900 μg of each CLASP1 protein was injected into a Superose 6 column (GE Healthcare) in a buffer containing 50 mM phosphate pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
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bioRxiv - Biophysics 2020Quote: ... the protein solution was fractionated using a DEAE Sepharose Fast Flow anion exchange chromatography resin (GE healthcare) eluting with a gradient from 0 to 1 M NaCl and further fractionated by size-exclusion chromatography using a HiPrep™ 26/60 Sephacryl® S-500 HR column ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The immuno-complexes were collected with 50 μl of protein A sepharose (17-0780-01, GE Healthcare) at 4 °C for two hours with rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoreactive protein bands were visualized via enhanced chemiluminescence and scanned with a LAS-4000 system (GE Healthcare).
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bioRxiv - Evolutionary Biology 2022Quote: ... The protein sample was further purified using a Superdex200 26/600 size-exclusion chromatography column (GE Healthcare), and fractions were collected and concentrated to 1.8 mg/ml in 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated and further purified on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: Equal amounts of protein were resolved on 10% SDS-PAGE and blotted onto nitrocellulose membranes (GE Healthcare). Immunoblots were blocked with 5% fat-free milk in PBS containing 0.1% Tween-20 for 1h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... ActRIIB-Fc was isolated using affinity chromatography with Mab Select Sure Protein A (GE healthcare - Waukesha, WI), followed by dialysis into 10mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The tagless protein was further purified by anion exchange chromatography (Mono Q 10/100 GL, GE Healthcare) in 25 mM potassium phosphate (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: Samples were precleared by adding 60 μl of 20% Protein A Sepharose™ CL-4B (GE Healthcare) in IP Buffer (15 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... the buffer exchanged protein was concentrated and injected on a SD200 10/300 increase column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Anti-TNFα IgG1 was purified on Protein A HiTrapHP columns (GE Healthcare Life Sciences, Little Chalfront, UK) using Akta-prime plus (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... partition coefficients of standard proteins from the high-molecular-weight gel filtration calibration kit (GE Healthcare #28403842) were plotted against Log(MW ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified using size exclusion chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) to remove nonspecific proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Collected fractions containing membrane proteins were loaded onto a Superose 6 increase 10/300 column (GE Healthcare) and eluted with 30 mL of NCMN Buffer E ...
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bioRxiv - Biophysics 2022Quote: ... the modified proteins were purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by size exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2022Quote: Samples were separated on SDS-polyacrylamide gel and proteins were transferred onto a nitrocellulose membrane (GE healthcare) using a wet transfer system (BioRad) ...
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bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
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bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were further purified by size exclusion chromatography on a Superdex200 16/60 column (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Microbiology 2021Quote: ... The approximate molecular weights of the loaded proteins were determined by molecular weight calibration kits (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the protein was concentrated and further purified by gel filtration using a superpose 6 increase (GE healthcare) in TBS containing 0.01% LMNG ...