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Citations for GE Life Sciences :
2701 - 2750 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the protein was further purified by size-exclusion chromatography on a Sephacryl 300 column (GE Healthcare) in PBS.
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
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bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... The protein was injected onto a Superdex-75 Increase 10/300 GL column (GE Healthcare 29148721) for size exclusion chromatography in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was loaded onto a HiLoad™ 16/600 Superdex™ 200 column (GE Healthcare) equilibrated with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected with enhanced chemiluminescence (ECL) detection reagents (RPN2236 or RPN2235, Amersham Biosciences, GE Healthcare,) and ChemiDoc™ XRS+ System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were separated by SDS-PAGE and transferred onto 0.45μm pore Protran nitrocellulose membrane (GE Healthcare) using semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Physiology 2024Quote: ... Target proteins were visualized by enhanced chemiluminescence using a commercially available kit (GE Healthcare, Freiburg, Germany).
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bioRxiv - Plant Biology 2024Quote: ... The bound GST-COI1 protein was detected using an anti-GST HRP conjugate (RPN1236, GE Healthcare, USA ...
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bioRxiv - Immunology 2024Quote: ... then further purified using Gammabind Plus Sepharose Protein G 25 mL column (GE Healthcare, Life Sciences), with 20 min contact time ...
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bioRxiv - Biochemistry 2024Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988) by amine coupling ...
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bioRxiv - Biochemistry 2024Quote: ... Protein for crystallization was further purified by size-exclusion chromatography on a Superdex 75 (GE Healthcare) with gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... The proteins separated on the gels were transferred to nitrocellulose membranes (Amershan Protan membranes, GE Healthcare) using transfer buffer (0.192 M Glycine ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins from the SDS-PAGE were transferred to a 0.45 µm nitrocellulose membrane (GE Healthcare, USA) at a current of 1 mA per cm² of SDS-PAGE for 45 min using the IMM-1-A semi-dry blotting system (The W.E.P ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysates were incubated with FLAG-Trap Protein-G Sepharose (GE Healthcare, Milwaukee, WI, USA) solution for 4 h ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred overnight onto nitrocellulose membranes (Whatman, GE Healthcare Life Sciences, Buckinghamshire, UK) in a 1 litre tank containing transfer buffer at 30 V.
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bioRxiv - Cancer Biology 2024Quote: ... mixed with 25 μl of protein A-Sepharose beads (GE healthcare, catalog no. 17-0469-01). The immunoprecipitated proteins were eluted from protein A-Sepharose beads by boiling with 2x sample buffer and subjected to SDS-PAGE.
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bioRxiv - Biochemistry 2024Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
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bioRxiv - Neuroscience 2024Quote: ... The protein samples were loaded into a Superdex 200 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with a buffer comprising 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions were then applied to a HiPrep- 16/60 Sephacryl S-300 HR (GE Healthcare) column equilibrated in TB+++ and RanGAP1- containing fractions were concentrated ...
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bioRxiv - Synthetic Biology 2024Quote: IgG/Fab protein was purified by size exclusion chromatography (SEC) using an ÄKTA system (GE Healthcare) equipped with a Hi-Load 16/60 Superdex 75 gel filtration column (GE Healthcare Life Sciences) ...
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bioRxiv - Bioengineering 2024Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer composed of 100 mM tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were purified by nickel affinity chromatography using a HisTrap FF pre-packed column (GE Healthcare). The column was equilibrated with the starting Buffer A ...
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bioRxiv - Bioengineering 2024Quote: ... The soluble protein fraction was then loaded on a HisTrap HP column (GE Healthcare, Chicago, USA) and His-tagged proteins were eluted with 500 mM imidazole in elution buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biophysics 2024Quote: Average molar masses and association states of proteins were determined from SEC (AKTA FPLC; GE Healthcare) coupled to multi-angle light scattering (DAWN ...
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bioRxiv - Cell Biology 2024Quote: ... the protein was subjected to size-exclusion chromatography using a S200 16/200 column (GE Healthcare) in 20 mM HEPES (pH7.4) ...
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bioRxiv - Biochemistry 2024Quote: ... and the untagged protein was collected by reverse IMAC on a HisTrap HP column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... or monocytes were isolated from buffy coat of healthy human donors (Biological Specialty Corp) by density centrifugation in Ficoll®-Paque PLUS (GE Healthcare Life Sciences). Monocytes were enriched either through positive selection using CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected using chemiluminescence with the ECL Prime Western Blotting Detection Reagent™ (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 12 % and 15 % gels followed by protein transfer onto nitrocellulose membranes (GE Healthcare Life Sciences, 0.45 µm). For better visibility of endogenous HA-hATG8s membranes were boiled for 5 min in PBS after protein transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pre-cleared with a 20 μl bed volume of protein-A sepharose beads (GE Healthcare) by end-over-end rotation for 1 hr at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Labeled protein was further purified on a Superdex 200 increase 10/300 gel filtration column (GE Healthcare) equilibrated in buffer B + 100 mM potassium acetate (KOAc) ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein bands were visualized using an Amersham ECL Advance Western Blotting Detection Kit (GE Healthcare, Germany).
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bioRxiv - Microbiology 2020Quote: ... ECL (Amersham) chemiluminescence substrates were used for protein detection using an ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The translated proteins were resolved using SDS-PAGE and analyzed by phosphorimager analysis (Typhoon, GE life sciences).
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins (GST-OsbZIP86 and MBP-OsSAPK10) were purified using Glutathione Sepharose 4B (GE Healthcare, USA) and Amylose Magnetic Beads (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... Separated proteins were then wet transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Science, 10600003) in Towbin buffer (25mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... Bands corresponding to various proteins were detected by corresponding antibodies using ImageQuant LAS 4000 (GE Healthcare, Tokyo) and analyzed using ImageQuant software ...