Labshake search
Citations for GE Life Sciences :
2651 - 2700 of 4122 citations for Cow Cathepsin L1 CTSL Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: ... The excess dye was separated from the labelled protein by SEC (Superdex 75 10/300 GL, GE Healthcare) performed in SEC buffer D (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... His6-tag free Ape1 protein was then loaded onto a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM Tris-HCl pH 7.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... cells according to the high titer protocol provided and purified on a Protein A HiTrap column (GE Healthcare) with the ACTA Pure FPLC system (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... the bromodomain was purified further using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare), equipped with a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare) ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was loaded onto a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) and purified using Assay Buffer or Assay Buffer without EDTA (for use in DNA cleavage assays) ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted protein was further purified using a Superdex Increase 10/300 GL column (GE Healthcare, Chicago, IL) equilibrated with buffer containing 350 mM NaBr ...
-
bioRxiv - Cancer Biology 2021Quote: ... We subjected equal amounts of proteins to SDS-PAGE and transferred them to nitrocellulose membranes (GE Healthcare #10401197). Non-specific binding was blocked by incubation with TBST plus 5% non-fat milk ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein was loaded on a HiPrep Q FF Anion Exchange Column (GE Healthcare Life Sciences, Marlborough, MA) and eluted by buffer with 300 mM NaCl ...
-
bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
-
bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
-
Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: SEC-MALS analysis of the purified proteins was performed on Superdex 75 10/300 GL column (GE Healthcare) using an Agilent HPLC system equipped with the 18-angle light scattering detector (Wyatt Dawn HELIOS II ...
-
bioRxiv - Microbiology 2021Quote: ... The protein complex was then purified by SEC using a Superdex 200 Increase 10/300 GL (GE Healthcare) column ...
-
bioRxiv - Cell Biology 2019Quote: ... for the detection of ubiquitinated protein aggregates or a 0.2 μm pore size cellulose acetate membrane (GE Healthcare) for the detection of huntingtin aggregates using a 96 well format Dot-Blot apparatus (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
-
bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
-
bioRxiv - Immunology 2020Quote: ... we used fritted deep well plates containing 25 μL of settled protein G resin (GE Healthcare Life Sciences) per well ...
-
bioRxiv - Molecular Biology 2021Quote: ... the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
-
bioRxiv - Immunology 2020Quote: ... fritted deep-well plates were used containing 25 μl of settled protein G resin (GE Healthcare Life Sciences) per well ...
-
bioRxiv - Immunology 2021Quote: ... IgG2 and IgG4 antibodies were isolated from cell supernatant using a HiTrap protein A column (Cytiva, GE Healthcare). IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva ...
-
bioRxiv - Microbiology 2021Quote: Proteins resolved by SDS PAGE were transferred to a nitrocellulose transfer membrane (GE Healthcare Amersham™ Protran™) at 80 V for 75 minutes or 15 V overnight at 4°C using a Bio-Rad wet transfer apparatus ...
-
bioRxiv - Immunology 2021Quote: ... The protein was further purified on a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare). SDS-PAGE was performed to determine the purity and size of the protein ...
-
bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
-
Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
-
bioRxiv - Plant Biology 2020Quote: Thylakoid proteins were separated by SDS-PAGE and then transferred to a polyvinylidene membrane (Hybond P, GE Healthcare) using a tank blot system (Perfect Blue Web M ...
-
bioRxiv - Cell Biology 2021Quote: ... protein extracts were subject to SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL). Primary antibodies used were anti-P2Y1 (Santa Cruz ...
-
bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Salt concentration is adjusted and protein is loaded on a 10 ml HiTrap Heparin HP column (GE Healthcare) equilibrated in 20 mM HEPES- KOH pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... All target proteins were subjected to size exclusion chromatography using the HiLoad 16/600 Superdex 75 (GE Healthcare) equilibrated in 25 mM Tris pH8.0 ...
-
bioRxiv - Cancer Biology 2022Quote: ... After protein fractionation by SDS-page samples were transferred to a nitrocellulose membrane (cat#10600002 from GE healthcare) and incubated with primary antibody solutions overnight at 4 °C (p21 ...
-
bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Total protein was loaded and separated in SDS-page gels and then transferred to PVDF membrane (GE Healthcare). For blocking ...
-
bioRxiv - Plant Biology 2022Quote: ... Protein was concentrated using a spin concentrator (Amicon, 10K MWCO) and injected onto Superdex 200 column (GE Healthcare) equilibrated in 25 mM HEPES (pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
-
bioRxiv - Microbiology 2022Quote: ... the protein was concentrated and loaded onto a Superdex 75 16/60 size exclusion (SEC) column (GE Healthcare). The peak corresponding to the protein was concentrated to about 12 mg/ml and flash frozen in liquid nitrogen and stored at −80°C.
-
bioRxiv - Microbiology 2022Quote: ... The supernatants containing antibodies were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: ... The protein solutions were further diluted with buffer B and eIF4A1 fractions subjected to a ResourceQ (GE Healthcare) anion exchange column ...
-
bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were concentrated and applied onto a Superdex 75 HiLoad 26/60 size-exclusion column (GE Healthcare) pre-equilibrated with gel-filtration buffer (10 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were then visualized by chemiluminescence reagent (Cyanagen(XLS3,0100),Thermo Fisher(34580)) using Amersham Imager 600 (GE Healthcare) and band densitometry was measured using FIJI software ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were visualized using the Amersham ECL Prime Western Blotting Detection System and Amersham Hyperfilm ECL (GE Healthcare). The following primary antibodies were used ...
-
bioRxiv - Cancer Biology 2024Quote: ... The supernatant was then incubated with 30 µl 50% protein A-Sepharose slurry (GE Healthcare, 17-0780-01) for 1 hr at 4 °C ...
-
bioRxiv - Neuroscience 2022Quote: ... The recombinant protein in the supernatant of the cell lysate was mixed with Glutathione Sepharose resin (GE Healthcare) and the mixture was washed extensively in 10mM HEPES pH 7.3 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Molecular weights were determined using pre-stained protein ladders (Thermo Fisher Scientific, catalog #26616; GE Healthcare, catalog #RPN800E). Protein abundance was detected on membranes using the SuperSignal West Femto enhanced chemiluminescence kit (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
-
bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
-
bioRxiv - Biophysics 2023Quote: ... the protein was subjected to size-exclusion chromatography using sephacryl S-100 HR 16/60 column (GE HealthCare). The final purified protein was concentrated to 1 mM using Amicon (3 kDa cutoff ...
-
bioRxiv - Neuroscience 2024Quote: ... The immuno-complexes were then incubated with 30 μl protein G sepharose beads (GE healthcare, 17-0618-01), which were pre-equilibrated with Lysis Buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 30 μl of a protein A/G sepharose mixture (GE Healthcare; #17-0780-01 and #17-0618-01), pre-equilibrated in ChIP dilution buffer was added to the lysates and incubation continued for 2 h at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... fritted deep-well plates were used containing 25 μl of settled Protein G resin (GE Healthcare Life Sciences) per well ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins were separated by 8-10% SDS-PAGE and transferred to a Hybond ECL nitrocellulose membrane (GE Healthcare) or Amersham Protran 0.45 μm NC nitrocellulose membranes (Carl Roth ...