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Citations for GE Life Sciences :
2901 - 2950 of 4122 citations for Cow Cathepsin L1 CTSL Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Excess DNA was removed from conjugated proteins using size exclusion chromatography on a Superdex200 10/300 column (GE Healthcare) in a 25 mM Tris buffer (pH 8 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein patterns in the polyacrylamide gels were recorded as digitized images using a calibrated densitometric scanner (GE Healthcare, USA) and analyzed (normalization ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The protein was then further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 (GE Healthcare) column using buffer III and a flowrate of 1 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... the M-Lipxtal protein was further purified by SEC using a HiLoad Superdex 75 26/600 column (GE Healthcare) equilibrated with SEC Buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The protein was further purified by size-exclusion chromatography on a Superdex 75 10/300 column (GE Healthcare, USA) equilibrated in buffer GF [20 mM Tris pH 7.0 ...
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bioRxiv - Genetics 2020Quote: ... Plasma lipoprotein particles were size fractionated by Fast Protein Liquid Chromatography (FPLC) using a Superose 6 column (GE Healthcare) and the cholesterol content in each fraction was measured accordingly26.
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bioRxiv - Neuroscience 2020Quote: ... Visualization of proteins was done by using Amersham ECL Western Blotting Detection Reagent and Amersham Hyperfilm (both GE Healthcare). Densitometric analysis of proteins was performed by ImageJ software ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentrated protein was further purified using HiLoad 16/600 Superdex-75 prep-grade gel-filtration column (GE Healthcare) pre-equilibrated with 20mM Tris HCl [pH 7.5] ...
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bioRxiv - Biochemistry 2020Quote: ... Twenty to 30 μg of proteins were separated by SDS-PAGE and then transferred to nitrocellulose membranes (GE Healthcare). Monoclonal anti-glutathione peroxidase 1 (GPx1 ...
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bioRxiv - Plant Biology 2019Quote: ... Samples containing 100 µg of protein were injected into a Superdex 200 increase 300/10 GL column (GE Healthcare) using a HPLC system (Ultimate 3000 ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Proteins were purified by size exclusion chromatography using Superdex200 10/300 GL column attached to AKTA purifier (GE Healthcare). Analytical gel filtration experiments were done using SHODEX Protein KW-800 GFC column (Showa-Denkko-KK ...
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bioRxiv - Immunology 2021Quote: ... The protein bands were detected using the enhanced chemiluminescence (ECL) Prime Western blotting detection system (GE HealthCare, Piscataway, NJ). The images of the blot were acquired on the ChemiDoc XRS using Bio-Rad ImageLab software (Bio-Rad laboratories ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was purified from the clarified supernatant using a Ni-NTA affinity chromatography column (HisTrap FF GE Healthcare) with binding buffer and eluted through on column cleavage with HRV 3C protease ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of the protein was estimated from the absorbance at 280 nm measured by a SimpliNano (GE healthcare) microvolume spectrophotometer with the extinction coefficient calculated by using the ExPASy ProtParam tool (51 ...
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bioRxiv - Physiology 2021Quote: ... The protein bands were detected using a chemiluminescence detection system (ImageQuant LAS 4000 mini; GE Healthcare, Little Chalfont, UK).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was harvested on day 5 and ACE2 protein was purified using HisTrap FF column (GE Life Sciences), followed by desalting ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the dye-labelled protein were detected by in-gel fluorescence imaging with an Amersham Typhoon (GE Healthcare) laser scanner.
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bioRxiv - Biochemistry 2021Quote: ... we further purified the proteins using a Superose™ 6 Increase 10/300 GL (GE Healthcare, 29-0915-96) size-exclusion column pre-equilibrated with 50 mM sodium acetate pH 4.2 ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: Protein samples were exchanged into a H-buffer using a PD-10 G-25 media desalting column (GE Healthcare) to induce droplet formation ...
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bioRxiv - Biochemistry 2020Quote: ... The protein samples were applied to a final size exclusion chromatography step using a Superose 6 column (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The protein and ligand were incubated for 10 min and spotted onto Protran BA85 nitrocellulose (0.45 μm, GE Healthcare) with a replicator tool (VP 408FP6S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was further purified with size-exclusion chromatography on HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) in NMR buffer ...
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bioRxiv - Immunology 2021Quote: The purification of 2H1 from NS0 cells consisted of affinity chromatography using rProtein A/Protein G GraviTrap (GE Healthcare). The antibodies were eluted with a 0.1 M glycine-HCL pH 2.7 solution and immediately neutralized with a 1 M Tris-HCl 1 M NaCl pH 9.0 buffer to maintain their stability in solution ...
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bioRxiv - Microbiology 2021Quote: ... size-exclusion (SEC) column connected to an ÄTKA™ pure fast protein liquid chromatography (FPLC) system (GE Healthcare Inc.). The column was equilibrated with SEC buffer (50 mM Tris ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... 100 μL of protein sample at 1 mg/mL was injected onto an S200 10/300 column (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Immunology 2021Quote: ... IgG from the hybridoma cell line supernatants was purified by affinity chromatography using protein G columns (GE Life Sciences). Purified H5.28 and H5.31 IgG generated from hybridomas was used for EC50 binding measurements ...
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bioRxiv - Neuroscience 2021Quote: ... Protein-antibody complexes were detected on X-ray films following addition of Enhanced Chemiluminescence (ECL) substrate (GE Healthcare, UK). The relative density of the pCREB and CREB bands was quantitated using ImageJ software (NIH ...
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bioRxiv - Biophysics 2020Quote: ... proteins were affinity purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast flow resin (GE Healthcare). Supernatant was two-fold diluted with 1xPBS (pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... for between 1 - 4 days and the protein signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Bioengineering 2022Quote: ... All proteins were further purified by size-exclusion chromatography using an S75 or a S200 Increase column (GE Healthcare), depending on protein size ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by size exclusion column (SEC) using HiLoad 26/600 Superdex 200 (GE Healthcare, USA) in SEC buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Eluted protein was further purified by size exclusion chromatography (SEC) using Superose 6 Increase 10/300 column (GE Healthcare), pre-equilibrated with the desired buffers.
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bioRxiv - Biophysics 2022Quote: ... Protein was further purified by size exclusion chromatography using a Superose 6 Increase column (10/300 GL, GE Healthcare) equilibrated with 150 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were visualized with the LAS-4000 system (ImageQuantTM LAS-4000, GE Healthcare Life Sciences, Pittsburgh, PA, USA) after a chemiluminescent reaction (Immobilon® Western Chemiluminescent HRP Substrate ...
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bioRxiv - Immunology 2022Quote: ... the protein-containing supernatant was purified by nickel-affinity and size-exclusion chromatography on an AKTAxpress system (GE Healthcare), which consisted of a 1 mL nickel affinity column followed by a Superdex 200 16/60 gel filtration column ...
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bioRxiv - Immunology 2022Quote: ... the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The eluted protein was concentrated and purified by gel-filtration chromatography (Superdex 200 10/300 GL, GE Healthcare, USA), using a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The cleaved protein was collected and further purified using gel filtration (HiLoad 16/60 Superdex pg 200, GE Healthcare) with GF buffer (20 mM Tris-HCl ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...