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Citations for GE Life Sciences :
2551 - 2600 of 4122 citations for Cow Cathepsin L1 CTSL Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified on a HiLoad 16/600 Superdex 75pg or 200pg gel-filtration columns (GE Healthcare). To obtain stoichiometric MLL1RWSAD complex ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... while the supernatant of GST-fusion protein homogenates were loaded onto pre-equilibrated glutathione SepharoseTM beads (GE healthcare), and incubated on an orbital rotator for an hour in cold room.
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were separated by SDS-PAGE and transferred to a polyvinylidene difluoride membrane (GE Healthcare Life science). Equal loading was validated using Coomassie Brilliant Blue staining of the membrane ...
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bioRxiv - Microbiology 2020Quote: ... All protein lysates were separated on 12% SDS-polyacrylamide gels and transferred to polyvinyl difluoride (PVDF, GE Healthcare) membranes ...
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bioRxiv - Plant Biology 2021Quote: ... pastoris X-33 and the secreted proteins were purified by affinity chromatography (HiTrap Chelating HP column) (GE Healthcare). Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Eluted protein was then applied to size-exclusion chromatography (Superdex 200 Increase 10/300 GL column, GE Healthcare) with the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Microbiology 2021Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL Western blotting detection reagent (GE Healthcare, #GERPN2209). In a parallel gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and GST-tagged protein were isolated on glutathione sepharose 4B resin (GE Healthcare) at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... 20X concentrated protein extract (10 ml) was loaded in an anionic-exchange Q-sepharose column (GE Healthcare, USA) previously equilibrated with 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Eight days post transfection the supernatant was loaded on the HiTrap protein G HP 1ml column (GE Healthcare) in binding buffer (20 mM sodium phosphate pH 7.0 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was analyzed by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (GE Healthcare, #BA85). Membranes were incubated for 1 h in a blocking solution (Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was then purified by gel filtration using a Superdex 200 26/600 GL column (GE Life Sciences) before cleaving the His6-SUMO tag with ULP1 enzyme ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were resolved using SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, cat#10600001) as previously described in [8] ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC; HiLoad 16/600 Superdex 200 pg, GE Healthcare) in 50 mM phosphate pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were further purified by size exclusion chromatography (Superdex-75 10/300 GL column; GE Life Sciences) and judged greater than 95% pure by SDS-PAGE analysis.
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bioRxiv - Cell Biology 2022Quote: ... and 0.1 mg protein were loaded on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare) connected to an ÄKTA pure (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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bioRxiv - Biochemistry 2022Quote: ... Concentrated protein for Cryo-EM was loaded over a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) using SEC Buffer (25mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... Then the protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Then the proteins were purified from the lysate with the Ni2+-NTA column of ÄKTA Pure (GE healthcare). Then the proteins were biotinylated using Avidity biotinylation kit and recommended protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified with gel filtration on a Hi-load 16/60 Superdex-75 column (GE Healthcare) in 25 mM Trizma (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was diluted with lysis buffer before application to a 5ml HiTrap STREP column (GE Healthcare), washed with lysis buffer and eluted using buffer supplemented with 2 mM Desthiobiotin ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was concentrated and applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in the same buffer.
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bioRxiv - Immunology 2022Quote: IgG was purified by separation over a HiTrap Protein A HP Antibody Purification Column (GE Life Sciences, 17040203) fitted to a GE Life Sciences Akta Purifier 10 HPLC ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins of interest were visualized with the Amersham ECL Detection Reagents (GE Healthcare by SIGMA, Cat. No. RPN2105) or LiteAblot TURBO Extra-Sensitive Chemioluminescent Substrate (EuroClone ...
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bioRxiv - Microbiology 2022Quote: ... The protein was then further purified by gel filtration using Superose 6 increase 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein solution was run at room temperature on a Superdex 75 10/300 GL (GE Healthcare, Piscataway, NJ) column with 2 mg/ml gD1 and a mobile phase composed of Tris- HCl at pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was run over a size exclusion column (GE Healthcare HiPrep 26/60 Sephacryl S-300 HR) in sizing buffer (50 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... protein digestion was concentrated and applied to a HiLoad™ 16/600 Superdex™ 75pg column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... 500 µl of freshly purified protein was loaded on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The P5CS proteins were further purified through HiLoad 16/600 Superdex 200 pg gel-filtration chromatography (GE Healthcare) in column buffer (25 mM HEPES pH 7.5 and 100 mM KCl) ...
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bioRxiv - Plant Biology 2021Quote: ... the purified protein was loaded on an analytical size exclusion column (SEC, superdex200 10/300, GE Healthcare, USA) pre-equilibrated with buffer containing 25mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: The purified protein was analyzed by Size Exclusion Chromatography (SEC) (Superdex 200 10/30 GL column; GE Healthcare) coupled with multi-angle light scattering (MALS ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare Life Sciences, Waukesha, WI), which were incubated at 4°C overnight with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments encoding APC proteins were inserted into pGEX-5X-1 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). For in vitro transcription and translation reaction ...
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bioRxiv - Cell Biology 2020Quote: ... Protein transfer from gel to membrane was performed with a 0.45 μm PDVF AmershamTM HybondTM membrane (GE Healthcare, formerly Amersham Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved DEPTOR protein was then collected and applied to a 5 mL HiTrap Heparin HP column (GE Healthcare) equilibrated with HEP-A buffer (50 mM HEPES pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was then injected on a High load superdex 200 16/600 gel filtration column (GE Healthcare) in deuterated PBS buffer pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified via gel filtration using Superose 6 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with the purification buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was divided equally and incubated with 50 μl of prewashed 50% Protein G slurry (GE Healthcare) for each condition ...
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bioRxiv - Immunology 2022Quote: ... Protein was concentrated and further purified by size-exclusion chromatography using Superose 6 16/60 (GE Life Sciences) in a PBS buffer ...