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Citations for GE Life Sciences :
2501 - 2550 of 5100 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Biotinylated proteins were purified using SEC on a Superdex 200 10/300 Increase GL (GE Healthcare) or S75 10/300 Increase GL (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were further purified by gel filtration on Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein detection was finally performed using Amersham ECL Western Blotting detection reagent (GE Healthcare #CA95038-564L).
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bioRxiv - Immunology 2023Quote: ... The proteins were visualized by ECL-Plus (GEHealthcare) using Image Quant LAS500 (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
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bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... 25µL of Protein L beads (Cat. no. Capto™ L resin, GE Healthcare Cat. no. 17547802), pre-equilibrated with binding buffer (20mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... the protein was further purified by size-exclusion chromatography on a Sephacryl 300 column (GE Healthcare) in PBS.
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
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bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... The protein was injected onto a Superdex-75 Increase 10/300 GL column (GE Healthcare 29148721) for size exclusion chromatography in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was loaded onto a HiLoad™ 16/600 Superdex™ 200 column (GE Healthcare) equilibrated with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... then further purified using Gammabind Plus Sepharose Protein G 25 mL column (GE Healthcare, Life Sciences), with 20 min contact time ...
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bioRxiv - Biochemistry 2024Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Scaffolds and RSVF trimer or IgG proteins were immobilized on a CM5 chip (GE Healthcare # 29104988) by amine coupling ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred overnight onto nitrocellulose membranes (Whatman, GE Healthcare Life Sciences, Buckinghamshire, UK) in a 1 litre tank containing transfer buffer at 30 V.
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bioRxiv - Cancer Biology 2024Quote: ... mixed with 25 μl of protein A-Sepharose beads (GE healthcare, catalog no. 17-0469-01). The immunoprecipitated proteins were eluted from protein A-Sepharose beads by boiling with 2x sample buffer and subjected to SDS-PAGE.
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bioRxiv - Biochemistry 2024Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein for crystallization was further purified by size-exclusion chromatography on a Superdex 75 (GE Healthcare) with gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected with enhanced chemiluminescence (ECL) detection reagents (RPN2236 or RPN2235, Amersham Biosciences, GE Healthcare,) and ChemiDoc™ XRS+ System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were separated by SDS-PAGE and transferred onto 0.45μm pore Protran nitrocellulose membrane (GE Healthcare) using semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Biochemistry 2024Quote: ... Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare). Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Physiology 2024Quote: ... Target proteins were visualized by enhanced chemiluminescence using a commercially available kit (GE Healthcare, Freiburg, Germany).
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bioRxiv - Plant Biology 2024Quote: ... The bound GST-COI1 protein was detected using an anti-GST HRP conjugate (RPN1236, GE Healthcare, USA ...
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bioRxiv - Microbiology 2024Quote: ... The proteins separated on the gels were transferred to nitrocellulose membranes (Amershan Protan membranes, GE Healthcare) using transfer buffer (0.192 M Glycine ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were subsequently incubated with the indicated primary antibodies followed by the appropriate horseradish peroxidase-labelled antibodies (1/10000, Sigma-Aldrich or GE Healthcare, see Supplementary table). Immunodetection was performed using Super Signal West Pico or Femto Chemiluminescence Kit (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected using chemiluminescence with the ECL Prime Western Blotting Detection Reagent™ (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... 12 % and 15 % gels followed by protein transfer onto nitrocellulose membranes (GE Healthcare Life Sciences, 0.45 µm). For better visibility of endogenous HA-hATG8s membranes were boiled for 5 min in PBS after protein transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pre-cleared with a 20 μl bed volume of protein-A sepharose beads (GE Healthcare) by end-over-end rotation for 1 hr at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Labeled protein was further purified on a Superdex 200 increase 10/300 gel filtration column (GE Healthcare) equilibrated in buffer B + 100 mM potassium acetate (KOAc) ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein bands were visualized using an Amersham ECL Advance Western Blotting Detection Kit (GE Healthcare, Germany).
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bioRxiv - Microbiology 2020Quote: ... ECL (Amersham) chemiluminescence substrates were used for protein detection using an ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The translated proteins were resolved using SDS-PAGE and analyzed by phosphorimager analysis (Typhoon, GE life sciences).
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins (GST-OsbZIP86 and MBP-OsSAPK10) were purified using Glutathione Sepharose 4B (GE Healthcare, USA) and Amylose Magnetic Beads (New England Biolabs ...