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Citations for GE Life Sciences :
2551 - 2600 of 4130 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was diluted to 70 mM NaCl and injected into an HiTrap Heparin HP 5 ml (GE Healthcare) column equilibrated with 20 mM HEPES pH 7.9 ...
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... The medium from 1 L of culture was filtered through a 0.22-μm membrane and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) in the presence of 20 mM of imidazole for 2-3 h at 4 °C with mild agitation ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 0.5 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2-3 hours on a roller ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then further purified by anion exchange chromatography using a 5 ml HiTrap Q HP column (GE Healthcare) across a gradient of 100 mM to 1M NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... The cleared supernatant was sterile filtered and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes of lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... The TEV-protease cleaved sample was loaded onto a 5 mL Q-Sepharose HP column (GE Healthcare Life Sciences), equilibrated in low-salt buffer and maintained at ∼4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biophysics 2020Quote: ... SEC-MALS was performed at room temperature by passing 500 μl protein (12μM) at 0.3 ml/min flow rate through a Superdex S200 SEC column (GE Healthcare) pre-equilibrated with 10mM potassium phosphate buffer supplemented with 150mM NaCl and 2mM β-mercaptoethanol (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed cleavage reactions were purified by chromatography on a 5 mL HiTrapQ Sepharose Column (GE Healthcare Life Sciences, USA) (or for SPARTIN ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were diluted with PBS to 10 ml and incubated with 200 μl of GST-Sepharose (GE Healthcare) for 1 hour on ice under agitation ...
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bioRxiv - Biophysics 2020Quote: ... 75 μL of 14.4 mg/mL IM30* were loaded on a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with 25 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2021Quote: ... The mixture was clarified by centrifugation at 90,000 x g and the supernatant was added to 0.5 mL StrepTactin Sepharose resin (GE Healthcare) per gram of cells and rotated at 4°C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2021Quote: ... 0.012% LMNG was concentrated up to 8 mg/ml using a 30 kDa MWCO Vivaspin 20 concentrator (GE healthcare). Extensive optimisation of crystal conditions using sitting-drop and hanging-drop vapour diffusion and other post-crystallisation treatments yielded plate shaped crystals after 3-4 days of incubation at 20°C ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 20 mM MOPS-NaOH pH 6.7 and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Samples were first fully submerged in 3 mL of Ficoll-Plaque Plus endotoxin-free water (GE Healthcare, Uppsala, Sweden) in either a 6-well culture plate or 3-mL test tube for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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bioRxiv - Immunology 2020Quote: ... Fractions corresponding to assembled SARS-CoV-2 S-I53-50NPs (8.5-9.5 mL) were collected and concentrated using Vivaspin columns with a molecular weight cutoff of 10,000 kDa (GE Healthcare). Protein concentrations were determined by the Nanodrop method using the proteins peptidic molecular weight and extinction coefficient as determined by the online ExPASy software (ProtParam).
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Secondary purification was performed by anion exchange chromatography using a HiTrap Q FF 5 mL prepacked column (GE Healthcare) installed on an Akta Start instrument (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare), and after washing with 10 column volumes (CV ...
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bioRxiv - Immunology 2020Quote: The stability of FPhtΔ3 under acidic conditions was evaluated by SEC on a 24 mL Superdex200 column (GE Healthcare) equilibrated in 20 mM HEPES ...
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bioRxiv - Physiology 2019Quote: ... A baseline aortogram with runoff was obtained with injection of 10 mL of Visipaque™ 320 (lodixanol; GE Healthcare) and C-arm fluoroscopy (GE OEC 9900 Elite).
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bioRxiv - Developmental Biology 2019Quote: ... which is spread across the glass bottom well before adding 1ul of thrombin (10 U/ml; GE Healthcare Lifesciences) to clot the fibrinogen ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was then applied to a HiTrap Q HP 5 mL anion exchange chromatography column (GE Healthcare) attached to an ÄKTA Explorer 100 system (Amersham Pharmacia Biotech) ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate 120 ml water samples were filtered serially through 2.7 μm Whatman GF/D (GE Healthcare, Little Chalfort, UK) and 0.22 μm Sterivex (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was further purified by cation-exchange chromatography on a 5 mL HiTrap S column (GE Healthcare) with a salt gradient of 0 to 1 M NaCl in 1×PBS supplemented with 1 mM DTT ...
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bioRxiv - Biochemistry 2021Quote: ... and those containing GsI-IIC RT were pooled and loaded onto a 5-ml Heparin HP column (GE Healthcare) pre-equilibrated in Heparin Buffer A (20 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was filtered (0.45 µm) and then loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 0.5 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2-3 hours on a roller ...
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bioRxiv - Immunology 2020Quote: ... Approximately 10mg of DH717.1 Fab was loaded onto a Superdex 200 increase 10/300 column using a 500μL loop and run at 0.5 mL/min using an Äkta Pure system (GE Healthcare). DH717.1 Fab peaks were collected via fractionation using a 96-well plate and were analyzed using the Unicorn 7.0.2 software (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: Samples of individual proteins and the different complexes were injected onto a PC3.2/30 (2.4 ml) Superdex 200 Increase gel filtration column (GE Healthcare) pre-equilibrated in 50 mM Tris pH 8.5 and 300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... and the membrane extract was loaded on a Chelating Sepharose Fast Flow bed resin (∼10 ml; GE Healthcare, UK) previously activated with 200 mM NiCl2 (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Following centrifugation at 18000 ×g the soluble fraction was applied to a 5 ml HisTrap HP column (GE Healthcare). The column was washed and protein eluted along a gradient using 20 mM Tris pH8 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of complement component C5 at 31.6 μM (5.96 mg/ml) was injected onto a Superdex 200 Increase 5/150 column (GE Healthcare) at a flow rate of 0.35 ml/min ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting sample was applied to a 120 ml HiLoad 16/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... the histones were purified from inclusion bodies under denaturing conditions on a 5 mL HiTrap SP FF (GE Healthcare) cation exchange column on a Next Generation Chromatograph (NGC ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was filtered (0.8 μM) and applied to a 10 or 20 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (lysis buffer without protease inhibitors) ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... The filtrate was applied to a 5 mL HisTrap HP Nickel Sepharose™ affinity column (GE Healthcare Life Sciences) and washed with five column volumes of binding buffer (50 mM sodium phosphate buffer ...