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Citations for GE Life Sciences :
2351 - 2400 of 4130 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and soluble form of VAMP-2 lacking the transmembrane domain (VAMP-21-96) were purified using a Mono S column (GE Healthcare, Piscataway, NJ) as described previously26 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: The Exosc3 open reading frame encoding the mouse EXOSC3 protein was cloned into pGEX-6P-2 plasmid (GE Healthcare Life Sciences (now Cytiva)) to create an N-terminally glutathione-S-transferase (GST ...
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bioRxiv - Cell Biology 2024Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Microbiology 2020Quote: ... The response data were fit to a 1:1 binding model using Biacore™ Insight evaluation software (GE Healthcare). For Fab competition experiments (Figure S8) ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM DTT at pH 7.0) while incubating with PreScission protease (protease to protein ratio 1:2000) (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody dilutions in 5% milk/PBST: anti-MBP HRP conjugated, 1:50,000 (NEB, and anti-GST HRP conjugates, 1:2,000 (GE Healthcare,). Scanned western blot films were quantified using the gel analysis tool in ImageJ.
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bioRxiv - Cancer Biology 2021Quote: ... and visualized using HRP-conjugated secondary antibodies (donkey anti-rabbit, 1:15,000, and sheep anti-mouse, 1:15,000, GE Healthcare) and ECL substrate (SuperSignal West Femto Maximum Sensitivity Substrate and Atto Ultimate Sensitivity Substrate ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). The equilibrium dissociation constants (binding affinity ...
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bioRxiv - Microbiology 2021Quote: ... Data were double reference-subtracted and fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Sensorgrams were fitted using 1:1 binding model and kinetic constants determined using Biacore T200 Evaluation software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). To determine the levels of nanobody competition with the human ACE2 and VHH72 of known epitope specificity ...
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bioRxiv - Microbiology 2023Quote: ... and a 1×10 cm strip of Whatman Grade 1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA). As the filter paper was the only carbon source ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA products from a 48-well PCR plate (50 - 100 µL reaction per well) were pooled and injected into an anion exchange column (MonoQ-GL, GE Healthcare Life Sciences, Marlborough, MA, USA) for purification ...
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bioRxiv - Biophysics 2021Quote: ... cell debris was removed and the supernatant loaded onto a 10 ml volume nickel-sepharose column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were then purified by cation exchange using a 5-mL HiTrap SP HP column (GE Healthcare) and the collected fractions analyzed by SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... were added at 25ul/ml to exclude immune and endothelial cells using MagRack 6 (GE Healthcare 89129-096). Cells were either frozen for future use or stained for flow cytometry ...
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bioRxiv - Cell Biology 2020Quote: ... The cleaved dCas9 protein was further purified with a 5 ml SP Sepharose HiTrap column (GE Life Sciences) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Plin3 AH was purified by anion exchange chromatography on a 20 ml Hiprep Q HP column (GE Healthcare). It was eluted with a salt gradient from 10 mM to 400mM NaCl (3 column volumes ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants containing the protein extracts were then applied onto a HisTrap FF 5 ml column (GE Healthcare) loaded with nickel ions using an ÄKTA Start liquid chromatography machine (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant containing complexes of Panx and Sov was loaded onto 5 ml HisTrap Fastflow column (GE Healthcare). After extensive washing with Binding buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... passed through a 0.22 μm filter and applied to a 20 mL HiTrap Heparin HP column (GE Healthcare). MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... 61236 × g for 20 min at 4 °C) 4 ml of Glutathione Sepharose 4B bead suspension (GE Healthcare) were added to the clarified lysate and incubated for 20 min at 4°C under constant shaking ...
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bioRxiv - Genomics 2019Quote: ... The diluted supernatants were applied to a 5 mL HiTrap heparin column (HiTrap™ Heparin HP, GE Healthcare) coupled to an ÄKTA purifier system (ÄKTA explorer ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification was done using an AKTA Purifier FPLC system on a HisTrap FF 5 ml column (GE Healthcare). The column was washed and equilibrated with 10 volumes of LB prior to sample loading ...
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bioRxiv - Synthetic Biology 2019Quote: ... The cleared lysate was incubated with 0.5 mL of a nickel sepharose resin (GE healthcare, Little Chalfont, UK), pre-equilibrated with 50 mM of KH2PO4 buffer (pH 7.5) ...
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bioRxiv - Biophysics 2022Quote: ... Supernatants were filtered by 0.45 μm and loaded onto a 5 mL HiTrap MabSelect SuRe column (GE Healthcare) equilibrated with COP Wash buffer (20 mM Hepes pH 7.4 and 500 mM NaCl) ...
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bioRxiv - Biochemistry 2021Quote: ... 30 min) and the supernatant was loaded onto a 5 mL HisTrap HP Ni Sepharose column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on a Ni-IMAC-HisTrap™ FF 5 mL column (GE Healthcare, Chicago, IL) at 3.5 mL/min ...
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bioRxiv - Neuroscience 2020Quote: ... The retentate was then applied to a Q Sepharose High-Performance column (GE Healthcare; 60-mL bed volume) equilibrated with FPLC-A buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the complex at reasonable purity were loaded onto a 5 ml HiTrap Heparin column (GE Healthcare), and the bound protein was eluted with a gradient from buffer A to buffer B (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... was used in conjunction with a HiLoad 16/60 Superdex 200pg 120 ml gel filtration column (GE Healthcare). The column was equilibrated with the required buffer containing the necessary detergent ...
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bioRxiv - Biochemistry 2021Quote: ... and then loaded on to a HiTrap™ SP Sepharose HP column (5 ml/min, GE Life Sciences) followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... supplemented with 20 mM imidazole and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes (CV ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixture was slowly added on the top of 6 mL of Ficoll (Ficoll-PaqueTM PLUS GE Healthcare) in a 15 mL conical tube ...
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bioRxiv - Plant Biology 2021Quote: ... 20X concentrated protein extract (10 ml) was loaded in an anionic-exchange Q-sepharose column (GE Healthcare, USA) previously equilibrated with 20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... with a Superdex S200 10/300 increase column at a flow rate of 0.75 mL/min (GE Healthcare) in PBS.
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were loaded at 0.5 ml/min onto a Superdex™ 200 Increase 10/300 GL (GE Healthcare) column with an ÄKTA™ Pure (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: Solubilized porins were loaded onto an anionic-exchange column (Hitrap HQ, 5 mL, GE Healthcare Life Science, France) and subjected to detergent exchange with 0.1 M MES buffer pH 6.5 complemented with 0.1% lauryldimethylamine-oxide (LDAO ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2022Quote: ... The top fractions after IMAC were pooled and desalted using a 5 ml HiTrap Desalting column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to 5 mL of equilibrated Glutathione Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were washed in a column 3 times with 50 mL of Buffer C (10 mM phosphate buffer pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... the α-Syn solution was subjected to anion exchange column (AEC) (HiTrap Q Hp 5 ml, GE healthcare) followed by size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2020Quote: ... with a Superdex S200 10/300 increase column at a flow rate of 0.75 mL/min (GE Healthcare) [Figure 1 and Supplementary Figure 1].