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Citations for GE Life Sciences :
2501 - 2550 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the protein was applied to a 5 ml HiTrap SP FF column (GE Healthcare Biosciences) and eluted with a linear gradient of NaCl (25-500 mM) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was detected by using ImageQuant™ LAS 500 imager (GE Healthcare, GENERAL ELECTRIC COMPANY).
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bioRxiv - Biochemistry 2020Quote: ... Resultant protein mixtures were analysed by ion exchange chromatography using HiTrap Q HP (GE Healthcare) and comparable samples from full elution profiles of wild-type and mutant proteins for each KASH binding-partner were analysed by SDS-PAGE ...
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bioRxiv - Bioengineering 2021Quote: ... The proteins were further purified by size exclusion chromatography (SEC) using Superdex 200 (GE Healthcare). The selected fractions were pooled and concentrated again for further use.
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bioRxiv - Bioengineering 2020Quote: All protein constructs were initially purified by IMAC on Nickel Sepharose Excel resin (GE Healthcare). 0.2 µm-filtered supernatants were applied to columns by gravity flow ...
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bioRxiv - Bioengineering 2020Quote: ... DBCO-functionalized nanobody was further refined on the fast-protein liquid chromatography (FPLC, GE Healthcare). DNA reporter conjugation was performed by incubating DBCO-functionalized nanobody (1 eq. ...
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bioRxiv - Biochemistry 2021Quote: ... The phospho-proteins were buffer exchanged using a Superdex75 10/300 GL column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was precleared with beads (Protein G sepharose™ 4 flast flow, GE Healthcare) for 1 hour at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using anti-SERCA2 antibody and protein A-coupled Sepharose beads (GE Healthcare). Beads were washed twice with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved protein was further purified through a Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Total protein was separated with SDS-PAGE gels and transferred to PVDF membrane (GE Healthcare). Membrane were blocked with 5% BSA in TBST for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... IgGs in supernatants were purified through a HiTrap protein A column (GE Healthcare, Chicago, IL). Antibodies were eluted with 0.1M glycine pH 2.7 ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was further purified by gel filtration (Superdex 200 10/300 GL, GE Healthcare) and concentrated to approximately 1.5 μM (Amicon) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then eluted using an AKTA Purifier 10 FPLC System (GE Healthcare, Fairfield, CT), with a gradient of 100 mM to 1 M NaCl in 20mM Tris buffer containing 5% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred on Amersham Protran Premium 0.2 μm nitrocellulose membranes (GE Healthcare, Solingen, Germany) a Bicine/Bis-Tris transfer buffer (25 mM Bicine ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins were transferred to the nitrocellulose membrane (GE Healthcare Life Sciences, Chicago, IL, USA), blocked with 3% BSA for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified on a size-exclusion Superdex200 Increase 10/300 GL column (GE Healthcare) in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified by anion exchange using an HP/Q Sepharose column (GE Healthcare) which was equilibrated with 2 column volumes (120 mL ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein complex was further purified on a HiTrap Heparin HP column (GE Healthcare), followed by size exclusion chromatography on a Superdex 200 10/300 GL equilibrated in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein supernatant was loaded onto a nickel-nitrilotriacetic acid (Ni-NTA) column (GE Healthcare) containing 0.1 M Ni2+ and equilibrated with the same loading buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was removed from unbound ligand by adsorption to Q-sepharose beads (GE healthcare, USA), the beads were washed ...
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bioRxiv - Biochemistry 2020Quote: ... These proteins were subsequently purified through gel permeation chromatography on Superdex 200 column (GE Healthcare) in Buffer E ...
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bioRxiv - Plant Biology 2020Quote: ... the wild-type and mutated AIPP3-BAH proteins were purified with glutathione-Sepharose (GE Healthcare) and eluted with elution buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then purified by ion exchange chromatography on a HiTrapSP HP column (GE Healthcare), equilibrated in 20 mM Citrate pH 5.0 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The target proteins were collected and desalted for subjecting MonoQ 4.6/100PE column (GE Healthcare) using Buffer A and C as shown in Fab proteins ...
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bioRxiv - Biophysics 2021Quote: ... The concentrated protein was loaded to an analytical grade size exclusion column (S75, GE Healthcare) for the further purification ...
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bioRxiv - Microbiology 2021Quote: ... Protein expression induction and purification was carried out similarly with Glutathione Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to Amersham Protran nitrocellulose membranes (0.45 µm pore size, GE Life Sciences) using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... SIL-proteins were purified using the Ni-Sepharose High-Performance resin (GE Healthcare Life Sciences) and precipitated using methanol:chloroform:water precipitation in Eppendorf Protein LoBind® tubes ...
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bioRxiv - Molecular Biology 2021Quote: ... The Ni-NTA affinity chromatography purified eLACCO1 protein was further applied on Superdex200pg (GE healthcare) size exclusion chromatography and buffer exchanged to TBS buffer supplemented with 1 mM CaCl2 ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were precleared using 20µl of protein G sepahrose beads (GE Healthcare, 7-0618-01) for 1hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then visualized by chemiluminescence using an ECL Western blotting detection system (GE Healthcare) and monitored by using a LAS-3000 imaging system (FujiFilm).
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were subsequently purified by anion exchange using a Mono Q column (GE Healthcare) in HB buffer (35 mM PIPES-KOH pH 7.2 ...
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bioRxiv - Biophysics 2021Quote: ... the resultant protein was further polished by a Superdex 200 gel filtration column (GE Healthcare). The purified protein was concentrated up to 60 mg/ml in buffer containing 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... and protein that contained flow-through was loaded onto a Heparin HP column (GE Healthcare). Rad2 was eluted from by a linear gradient of heparin buffer A (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... This solution was loaded onto a 5 ml HiTrap Protein G HP column (GE Healthcare) equilibrated with 20 mM Na3PO4 pH 7 and eluted with 0.1 M glycine-HCl pH 2.7 ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were directly loaded onto anion exchange chromatography (HiTrap Q HP 5ml column; GE Healthcare). The column was equilibrated with 4 CV gradient wash from 0–20 % buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: CEL protein variants were purified by HiTrap Heparin HP Affinity Column (GE Healthcare Life Sciences) controlled by an ÄKTA pure protein purification system as described previously (20) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified by size-exclusion chromatography using a Superdex 200 column (GE healthcare) in SEC buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The Fab and IgG proteins were purified from cell supernatants by Ni2+-NTA (GE Healthcare) and protein A affinity chromatography (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The ITC protein sample p52 (1-398) went through desalting column (HiTrap desalting, GE Healthcare) to freshly made ITC buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2022Quote: ... the protein was further purified on a Superdex 200 HiLoad 16/600 column (GE Healthcare) in gel filtration buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The Ulp1 treated protein sample was loaded directly onto a prepacked GSTrap column (GE Healthcare). Protease 3C was added to the eluted GST-AAGAB:AP2α binary complex to cleave the GST tag ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was then injected onto a 16/600 or 10/300 Superdex200 column (GE Healthcare) equilibrated with gel filtration buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed via affinity chromatography using HisTrap HP or GSTrap FF (GE Healthcare) based on the tag expressed in the N-terminal part of the protein ...
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bioRxiv - Neuroscience 2022Quote: Proteins were transferred from the acrylamide gel onto the nitrocellulose membrane (0.22 μm, GE Healthcare). Membranes were incubated with the primary antibodies (α-TSPAN5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was further purified on a Mono S 10/100 GL column (GE Healthcare) using a 100–400 mM KCl gradient in 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The dialyzed protein was passed through the equilibrated Q-Sepharose Fast Flow beads (GE Healthcare). The beads were washed with dialysis buffer 1 with gradual increase in NaCl concentrations 40 mM ...