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Citations for GE Life Sciences :
2401 - 2450 of 4765 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Proteins in the supernatant were further purified by HiTrap Heparin HP column (GE Healthcare) with A Buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... Marker proteins were from the isoelectric focusing calibration kit (high pH range, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was further purified using a Superose 6 increase column (GE Healthcare) pre-equilibrated with the binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... GST fusion proteins or GST alone were bound on glutathione-Sepharose resin (GE Healthcare) and incubated with 5 μg of purified Snu71 or BBP protein in pull-down buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... GP63 and α-Tubulin was performed by transferring proteins to nitrocellulose membranes (GE healthcare). The membranes were blocked ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE HealthCare) and stained with Ponceau red ...
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bioRxiv - Microbiology 2023Quote: ... the filtered protein-nanodisc solution was purified using a Superose 6 column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was purified by anion exchange chromatography on a HiTrapQ column (GE Healthcare) via a linear NaCl gradient and twice by size exclusion chromatography using a Superdex S200 26/60 column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of anti-HA monoclonal antibodies crosslinked to protein G MicroBeads (GE Healthcare) were added to the cleared lysate and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare®) on an iBright FL1500 (ThermoFisher Scientific®).
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein fractions were run through a desalting column (PD-10, GE Healthcare), the buffer was exchanged into 3 mL ion-exchange Buffer A (50 mM Bis-tris pH 7.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The eluted protein was further purified on a StrepTrap HP affinity column (GE Healthcare) eluted using 10 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein bands were detected using an Amersham Imager 600 UV (GE Healthcare, Eindhoven, Netherlands) and visualized using ImageJ-Fiji software (version 1.53t ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were concentrated to 10 mg/mL using vivaspin 10 MWCO concentrators (GE healthcare). Crystallisation screens were performed by sitting drop vapour diffusion using 3 Lens Crystallisation Plates (SWISSCI ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were applied onto a Superose 12 10/300 GL column (GE Healthcare), which had been pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2024Quote: ... Refolded proteins were further purified on a Superdex-200 16/600 column (GE Healthcare) using 20 mM TrisHCl [pH 8.0] ...
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bioRxiv - Microbiology 2024Quote: ... The GST fusion proteins were affinity purified via glutathione-sepharose (GE Healthcare, Dornstadt, Germany) by gravity flow ...
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bioRxiv - Genetics 2024Quote: ... Chromatin fragments bound to antibody were captured on Protein A-Sepharose beads (GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were wet transferred overnight onto a 0.45-μm nitrocellulose membrane (GE Healthcare) in ice-cold Towbin buffer at 4°C ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMC) were enriched by density gradient centrifugation of peripheral blood from healthy human donors through a Ficoll-Paque PLUS (GE Healthcare) gradient ...
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bioRxiv - Biochemistry 2019Quote: Human BRG1 AT-BD (residues 1434–1569) or BD (residues 1454-1569) were cloned into a pGEX6p-1 vector (GE Healthcare) from full length BRG1 cDNA obtained from GE Open Biosytems ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Human PBMCs were isolated from leukopheresis packs obtained from the Central Blood Bank (Pittsburgh, PA) via Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... lymphocytes and monocytes were isolated from heparinized human peripheral blood by Ficoll-PaqueTM PLUS (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) gradient centrifugation ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... was constructed by inserting the cDNA for human SSH1 (869–1049) into the BamHI and NotI sites of pGEX-4T (GE Healthcare). E ...
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bioRxiv - Biochemistry 2023Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) and human ZIP8 (GenBank access number: BC012125) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... The labelled protein was applied to a Thiopropyl Sepharose 6B column (GE Healthcare Life Sciences) to remove double labelled protein ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was then loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare), washed with 5 CV of Heparin Wash Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein was purified by Superose6 Increase 10/30 (GE Life Sciences, Cat. #29091596) gel filtration.
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bioRxiv - Biophysics 2020Quote: ... The protein solution was then loaded onto a Superdex 200 10/300 column (GE Healthcare). The peak fractions were pooled and concentrated (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were passed over an either HisTrap or GstTrap FF 5□ml column (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Diluted proteins were subsequently passed over a HiTrap Heparin HP 5□ml column (GE Healthcare). After washing with binding buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and immune complexes were captured using the Protein G Sepharose (17-0618-01, GE Healthcare). Samples for gel electrophoresis were prepared in Laemmli Sample Buffer (LDS ...
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bioRxiv - Cell Biology 2020Quote: ... overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... HRP-labeled proteins were detected with Amersham ECL Western Blotting Detection Reagent (GE Healthcare, UK) or Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... along with 5µL of protein ladder (Full-Range RPN800E, GE Healthcare Rainbow Molecular Weight Markers). After transfer ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...