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Citations for GE Life Sciences :
2501 - 2550 of 3174 citations for Anti Cullin 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured by Bradford assay and 20 µg of denatured protein (in Laemmli buffer at 95°C for 5 min) was loaded in a 7.5% polyacrylamide gel and blotted onto a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK). The membranes were blocked in 5% milk in PBS with 0.01% Tween (PBST ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting ABC holotoxins were then separated from excess TcB-TcC by size exclusion chromatography on a Superose 6 increase 5/150 column (GE Life Sciences) equilibrated in labelling buffer ...
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bioRxiv - Biochemistry 2023Quote: The supernatant containing the target protein was then loaded on a 5 mL Ni-NTA column followed by a size exclusion column (Superdex 75, GE Healthcare, USA). YsiA is then eluted stepwise with 30 mM ...
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bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 3×10mins with TBS-T and 1×10mins with TBS before developing the signal by incubating the membrane in Amersham Hyperfilm ECL for 5 minutes (GE Healthcare, UK).
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Improved Activity and Kinetics of Endoglucanase Biofuel Enzyme with Addition of an AzoTAB SurfactantbioRxiv - Bioengineering 2023Quote: ... The protein solution was filtered and applied to a 5 mL HiTrap Q HP anion exchange column (GE Healthcare, Cat. no. 17115301) and later washed with 30× column volume with the protein buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the combined fractions were subjected to anion-exchange chromatography on two connected 5 ml HiTrap Capto Q ImpRes (GE Healthcare Life Sciences) anion-exchange columns using a linear gradient ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The supernatant was syringe-filtered to 0.22 µm and then purified using a 5 mL HisTrap FF nickel affinity column (GE Healthcare, Piscataway, NJ). Fractions were eluted with an imidazole buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2023Quote: Human packed RBC were spin washed (1800 rpm; 5 minutes) three times with chilled 1X PBS and resuspended in RPMI (HyClone, GE Healthcare, USA) at 2% haematocrit ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysates were filtered through a 0.45 µm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with wash buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Imidazole was added to the supernatant to final concentration of 10mM loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare) equilibrated in NiNTA A buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysates were filtered through a 0.45 μm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with 5 CV of wash buffer (20 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... The cell lysates were filtered through a 0.45 μm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with 5 CV of wash buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Purity and molecular weights were analyzed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analytical size exclusion chromatography (SEC) using a Superdex™ 75 5/150 GL column (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing Fibril Δ(273-512)-His6 was loaded into a pre-packed 5 mL Ni-NTA column (HisTrap; GE Healthcare) equilibrated with binding buffer ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting lysate was filtered through a 0.2 μm nylon filter and loaded onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences) that had been equilibrated in Buffer A ...
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bioRxiv - Bioengineering 2024Quote: ... beads were suspended in 0.22 mm filtered PBS with 5 ml (10 units) of PreScission protease (catalog # 27084301; GE healthcare Life Sciences) and incubated overnight at 4°C on a rotospin test tube rotator for on-column PreScission protease cleavage ...
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The antibody was pre-incubated with 20 μl of prewashed protein G Sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies were immobilized using direct immobilization using amine coupling on CM3 sensor chips (GE Healthcare) at ∼5000 RU ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected using horseradish peroxidase-coupled secondary antibodies (GE Healthcare Life Sciences, Piscataway, NJ) and ECL Plus or ECL Prime western blotting detection reagents (GE Healthcare Life Sciences) ...
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bioRxiv - Biophysics 2019Quote: ... used at 1:5000 dilution and detection with peroxidase-labelled secondary antibodies (GE Healthcare, UK) and ECL Plus (enhanced chemiluminescence ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Bioengineering 2021Quote: ... The antibody secreted into the culture medium was purified using Protein A resin (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were detected using ECL™ Western Blotting Detection Reagents (RPN2209, GE Healthcare, IL, USA). As a control ...
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bioRxiv - Cancer Biology 2020Quote: ... the antibody-bound membranes were treated with enhanced chemiluminescent western blot detection reagents (GE Healthcare) and visualized with an x-ray film (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... secondary antibodies conjugated with HRP and ECL Prime detection reagents were acquired from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies at 1: 5000 dilution (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... the antibody was pre-incubated with 20 μl of prewashed protein G sepharose (GE healthcare) at 4°C for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... or with HRP-conjugated secondary antibodies and ECL Prime Western Blotting System (#RPN2106, GE Healthcare) and imaged using a ChemiDoc imaging system (Biorad) ...
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bioRxiv - Bioengineering 2020Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse monoclonal antibodies were purified from hybridoma supernatant using protein G columns (GE Healthcare). The specificity of CAST monoclonal antibodies was confirmed by immunohistochemistry with CAST KO mice (56) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins recognised by the antibodies were visualised using ECL prime (GE Healthcare, Chicago, IL, USA) and digital images were acquired with a LAS4000 mini imager (FUJIFILM Wako Pure Chemical ...
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bioRxiv - Cell Biology 2019Quote: ... Antibody-conjugated proteins were collected by incubating with Protein A Sepharose beads (17528001, GE Healthcare). Beads were then washed 4-5 times with the lysis buffer described above ...
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bioRxiv - Immunology 2019Quote: ... followed by HRP-conjugated secondary antibodies and detection with chemiluminescent substrate solution ECL2 (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... α-rabbit IgG secondary antibody conjugated to HRP (from donkey) was purchased from GE Healthcare and used at 1:5,000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated for two hours with the corresponding HRP conjugated secondary antibody (GE Healthcare). After incubation with the secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:4000) for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified from serum-free culture supernatants using mAb Select SuRe resin (GE Healthcare) followed by rapid desalting using a HiPrep 26/10 Desalting column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... IgG1 antibodies were isolated from cell supernatant using a HiTrap protein A column (GE Healthcare) and Äkta Pure protein chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... we added 100 µl/well of Amersham ECL-HRP linked mouse IgG antibody (GE Healthcare) diluted 1/10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with secondary antibodies and visualised using an ImageQuant LAS 4000 imaging system (GE Healthcare). A list of antibodies used for immunoblotting is provided in Supplementary Table1 ...
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bioRxiv - Cell Biology 2021Quote: ... Blots stained with AF-conjugated secondary antibodies were imaged using a Typhoon Imager (GE Healthcare) or a BioRad ChemiDoc ...
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bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...