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Citations for GE Life Sciences :
2651 - 2700 of 3174 citations for Anti Cullin 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Synthetic Biology 2024Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5 ml column, GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Immunology 2021Quote: Purification of the recombinant antibodies was conducted on a ÄKTAprime plus system (GE Healthcare Life Sciences) at 4°C using a 1 mL HiTrap® Protein A HP pre-packed Protein A Sepharose® column (Cytiva) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the bound antibodies were visualized by chemiluminescence using the ECL plus reagent (GE Healthcare, Buckinghamshire, UK). The GAPDH content was analyzed as the loading control using a rabbit polyclonal antibody.
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were detected using mouse IgG HRP linked whole Ab (1:4000; NXA931; GE healthcare).
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bioRxiv - Cancer Biology 2019Quote: ... or a combination of the appropriate antibody and Protein A or G sepharose beads (GE Healthcare) were used to immunoprecipitate tagged or endogenous proteins from cleared lysates ...
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bioRxiv - Biochemistry 2020Quote: ... samples were incubated with appropriate antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) or Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with Horseradish Peroxidase (HRP)-conjugated secondary antibody (GE healthcare, NXA931V or NA934V) in 1% non-fat BSA in PBST at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1mg of purified antibody was injected into the AKTA protein purification system (GE Healthcare Life Sciences) and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... Secondary horseradish peroxidase (HRP)-conjugated antibody dilutions were used as follows: goat α-rat (GE Healthcare) 1:2,000 ...
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bioRxiv - Systems Biology 2020Quote: ... The following secondary antibodies were used for immunoblotting: ECL rabbit HRP-linked IgG (GE Healthcare, NA934) and ECL mouse HRP-linked IgG (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated with HRP conjugated secondary antibody (IgG; mouse or rabbit) (GE Healthcare UK) at 1:5000 dilution in blocking buffer and incubated at RT with shaking for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were washed and immunoreactive bands were developed for five minutes with ECF (GE Healthcare) and revealed in a Chemidoc Imaging System (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... either a 1:10,000 dilution of α-mouse-HRP conjugated antibody (GE Healthcare Life Sciences NA931) or a 1:2,000 dilution of α-mouse-HRP conjugated antibody (used for western blot of immunoprecipitations ...
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bioRxiv - Cell Biology 2022Quote: ... antibody-antigen complexes were detected by FUSION SOLO S (VILBER) using an ECL system (GE Healthcare). Quantification was performed via densitometry using Fusion Capt software (VILBER) ...
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bioRxiv - Molecular Biology 2020Quote: ... and then incubated for 1 h with ECL mouse IgG HRP-conjugated whole antibody (GE Healthcare) or rabbit IgG HRP-conjugated whole antibody (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... the antibodies secreted into the supernatant were collected and captured by protein A Sepharose (GE Healthcare). The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... The binding of HRP-conjugated secondary antibodies was revealed by ECL Plus (GE Healthcare Life Sciences) and chemiluminescence was measured using a ChemiDoc XRS apparatus (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Genetics 2019Quote: ... and the secondary antibodies were visualized by ECL Prime Western Blotting Detection Reagents (GE Healthcare, UK).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBST before incubation with secondary HRP-conjugated antibodies (GE Healthcare). Membranes were further washed three times with TBST before ECL exposure.
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bioRxiv - Immunology 2020Quote: ... The secreted antibodies were subsequently purified from the supernatant on HiTrap Protein G columns (GE Healthcare). Plasmid DNA encoding a gliadin-reactive IGHV4-4:IGKV4-1 antibody with R in position 55 (corresponding to the IGHV4-4*07 allele ...
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bioRxiv - Immunology 2019Quote: ... Antibody binding was visualized with Amersham ECL Western blotting detection reagent (GE Healthcare, Little Chalfont, UK).
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bioRxiv - Cell Biology 2019Quote: ... Signals were detected with HRP-conjugated secondary antibodies and visualized with ECL detection reagent (GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... membrane was incubated with horseradish peroxidase-conjugated secondary antibodies before analyses by enhanced chemiluminescence (GE Healthcare). B-Actin was used as loading control.
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bioRxiv - Neuroscience 2021Quote: ... followed by 1 h at room temperature in the appropriate HRP-labelled secondary antibody (GE Healthcare). Blots were developed with ECL Clarity (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was concentrated and purified over HiTrap Protein G HP antibody purification column (GE Healthcare). To visualize 410C9 in vivo ...
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bioRxiv - Immunology 2020Quote: ... The washed membrane was incubated with a secondary antibody labeled with horseradish peroxidase (HRP) (GE healthcare) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies and the ECL prime western blotting system (GE Healthcare, RPN2232) were used for detection of primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: Antibodies against CFTR were pre-incubated with protein-A or protein-G Sepharose beads (GE Healthcare) for 15 minutes at 4°C before adding protease-treated or non-treated lysates ...
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bioRxiv - Plant Biology 2023Quote: ... The antibodies were purified from rabbit serum by affinity chromatography on CH-Sepharose 4B (GE Healthcare) coupled to 6His-CPK3.
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies: 1:10000 goat α rabbit or goat α mouse ECL (GE healthcare NA931, NA934).
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bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...
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bioRxiv - Developmental Biology 2023Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies and the ECL prime western blotting system (GE Healthcare, RPN2232) were used for detection of primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
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bioRxiv - Immunology 2024Quote: Monavalent SPR of CD19 antibodies was conducted on BI-Acore T200 instrument (GE Healthcare Life Sciences). The experiment was run at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The antibody binding was visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare; #RPN2236) and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies were used as follows: Horseradish peroxidase (HRP)-conjugated mouse IgG (1:10,000; GE Healthcare) or rabbit IgG (1:3000 ...
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bioRxiv - Biochemistry 2019Quote: ... The reacting agents were then separated from the now methylated proteins by size-exclusion chromatography using NapTM-5 columns according to the manufacturer’s instructions (GE Healthcare Life Sciences, Amersham, UK).
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The culture medium containing the secreted ACE2 was subsequently loaded onto an ion-exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.), and subsequently eluted by a linear salt gradient ranging between 10 and 1000 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Genomics 2020Quote: ... The mixture was left to react for 30 minutes protected from ambient light and the crude reaction mixture was passed through a NAP-5 column (GE Healthcare Life Sciences, 17085301) for collection and purification of the fluorophore-conjugated secondary antibody ...