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Citations for GE Life Sciences :
2401 - 2450 of 3174 citations for Anti Cullin 5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase conjugated Goat anti-Mouse IgG (GE Healthcare) diluted 1:5000 was used as secondary antibodies for the detection of Mouse IgGs.
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bioRxiv - Neuroscience 2021Quote: ... and sheep anti-mouse (NA931, GE Healthcare, 1:10000). The guinea-pig anti-KCTD16 antibody was raised against a synthetic peptide derived from mouse KCTD16 (aa7-23 ...
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bioRxiv - Molecular Biology 2020Quote: ... and HRP-linked anti-Mouse IgG (NA931, GE Healthcare) were used as secondary antibody ...
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bioRxiv - Genomics 2020Quote: ... HRP-conjugated anti-mouse (GE Healthcare NA931V, 1:10000) and HRP-conjugated anti-rabbit (Santa Cruz sc-2004 1:5000 ...
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bioRxiv - Microbiology 2019Quote: ... sheep anti-mouse HRP-linked IgG (GE Healthcare, UK) for Western immunoblotting and rat anti-mouse IgG H+L conjugated with FITC for FACs analysis (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... followed by secondary HRP-conjugated anti-mouse (GE Healthcare) or anti-rabbit (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rabbit-IgG-F(ab’)2 (GE Healthcare) antibody for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse (GE Healthcare Cat# NA931, 1:5000 dilution), and then detected using an ECL SuperSignal West Femto y West Pico detection kit (Pierce).
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bioRxiv - Cell Biology 2022Quote: ... HRP conjugated donkey anti-rabbit IgG (GE Healthcare, NA934V), 1:10000 ...
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bioRxiv - Neuroscience 2019Quote: ... or anti-rabbit HRP-conjugated (1:20,000; GE Healthcare) in blocking solution for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... or anti-rabbit (GE Healthcare Life Sciences, NA934V/AG) antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and anti-rabbit conjugated HRP (1:500; GE Healthcare) as secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated with goat anti-GST (GE Healthcare) for an hour and GST binding was detected kinetically using an alkaline-phosphatase-linked rabbit anti-goat IgG secondary antibody (diluted 1:2000 in 0.1% BSA/PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... and HRP-anti mouse secondary (GE Healthcare; 1:5000). Western blots were developed on X-ray film using a SRX-101A table top film processor.
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bioRxiv - Microbiology 2020Quote: ... and sheep anti-mouse IgG (GE Healthcare, 1:2000) HRP-conjugated secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit IgG HRP (1:5000 GE Healthcare NA934V), anti-mouse IgG HRP (1:5000 Abcam ab97046).
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bioRxiv - Microbiology 2021Quote: ... HRP-conjugated anti-mouse (NA931, GE Healthcare, IL USA), anti-rabbit (NA934 ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-mouse IgG (1:10,000; NXA931V; GE Healthcare).
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bioRxiv - Neuroscience 2022Quote: ... or sheep anti-mouse IgG (GE Healthcare, 1:10,000) in TBS-T containing 5% milk for 2 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... using anti-Rabbit HRP-conjugated (1:5000, GE Healthcare) as a secondary antibody ...
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bioRxiv - Physiology 2024Quote: ... and sheep anti-mouse (GE Healthcare #NA931V, 1:1000). Protein bands were detected using Chemiluminescent Substrate (Thermo Fischer ...
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bioRxiv - Immunology 2024Quote: ... or peroxidase-conjugated anti-rabbit IgG (#NA934; GE Healthcare), were added as the secondary antibodies to the buffer in which the membrane was soaked ...
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bioRxiv - Biochemistry 2023Quote: ... or donkey anti-rabbit (1:10,000; GE Healthcare #NA934V).
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bioRxiv - Biochemistry 2023Quote: ... The anti-mouse M13-HRP (GE Healthcare Cat# 27942101) was added at 1:5000 dilution for 1hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-rabbit (GE Healthcare # NA9340, dilution 1:10,000) origin and ECL substrate (Thermo Scientific # 32106) ...
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bioRxiv - Immunology 2023Quote: ... or anti-rabbit IgG (for IgT detection; GE Healthcare), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... and donkey anti-rabbit IgG (Cell signaling, GE Healthcare) used in a dilution of 1:10000 and incubated at room temperature for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... and goat-anti-rabbit were obtained from GE HealthCare/Amersham ...
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bioRxiv - Biochemistry 2023Quote: ... the secondary mAB anti-mouse (sheep, GE Healthcare, LNA931V) and developed using Clarity Western ECL Substrate (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed with 1x TTBS and placed in a 1:25000 secondary HRP-conjugated IgG anti-rabbit or anti-mouse solution (GE Healthcare®, Sweden) according to the primary antibody datasheet specifications ...
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bioRxiv - Cancer Biology 2021Quote: ... a cell suspension containing 20,000 cells per 50uL was prepared in Biolog IF-M1 containing 0.3mM glutamine and 5% dialyzed FBS (dFBS) (Hyclone GE Life Sciences, SH30079.01) and plated into PM-M1 and PM-M2 96-well plates at 50 μL and 20,000 cells per well ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
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bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
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bioRxiv - Genetics 2019Quote: ... 293T cell line was cultured in DMEM high-glucose medium with 5% HyClone® fetal bovine serum (GE Healthcare, Piscataway, NJ, USA) and were maintained at 37°C with 5% CO2.
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bioRxiv - Biochemistry 2019Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 10/300 column or on a Superose 6 5/150 column (GE Healthcare, Germany) in gel filtration buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Neuroscience 2019Quote: ... α-syn was centrifuged at 12000g for 5 minutes and the supernatant loaded onto a Superdex™ 200 10/300 GL column (GE Healthcare). The column was eluted with PBS at a flow rate of 0.5 mL/minute ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
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bioRxiv - Cell Biology 2021Quote: ... Figure 5F and Figure 5-figure supplement 1D were acquired and processed by iterative deconvolution using Soft-WoRx software (Applied Precision Ltd.). For Figure 1E ...
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bioRxiv - Bioengineering 2021Quote: ... The dialyzed eluate was further passed through an equilibrated 5 mL heparin column (HiTrap™ Heparin HP) on a FPLC (AKTA pure, GE healthcare) and eluted using a linear NaCl gradient generated from heparin buffers A and B (40 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare). Fractions containing protein of interest were pooled ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Purification was performed by combing a HisTrapFF column and analytical size-exclusion chromatographic Superdex™ 75 5/150 with an ÄKTAexplorer 100 (GE Healthcare). The human cDNA sequence for the HIF-1α-PAS-B domain was synthesized and subcloned into the prokaryotic expression vector pGEX-4T-1 (Amersham) ...
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bioRxiv - Biochemistry 2020Quote: ... Both the LaGH13_20 and LaGH13_31B enzymes were purified by immobilized metal ion affinity chromatography on 5 ml HisTrap HP columns (GE Healthcare, Uppsala, Sweden) equilibrated with binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... diluted two-fold against fresh buffer B and then manually applied at room temperature to a 5 mL HiTrap Heparin cation exchange column (GE Healthcare # 17040701) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purification from precipitated cell-culture media was done on HisTrap HP 5 ml and HiLoad 16/600 Superdex 75 (GE Healthcare, USA) columns by the method described previously (Zahradník et al. ...
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bioRxiv - Biophysics 2021Quote: ... Bacterial cells were disrupted by sonication and the soluble fraction was loaded on a 5 mL HiTrap Chelating HP column (GE Healthcare - USA) previously equilibrated with same buffer ...