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Citations for GE Life Sciences :
2451 - 2500 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged or MBP-tagged proteins were captured by glutathione-agarose resin (GE Healthcare, 17075601) or amylose resin (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... the RTL4CV protein was detected with an ECL Prime Western Blotting Detection kit (GE Healthcare) using an anti-GFP antibody (MBL ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred at 70 V for 2 h onto a nitrocellulose membrane (GE Healthcare) using a wet electroblotting system (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... protein spots were transferred to Amersham Hybond-P poly(vinylidene difluoride) (PVDF) membranes (GE Healthcare) and the Western Blot (WB ...
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bioRxiv - Immunology 2024Quote: ... The eluted proteins were further gel filtered using a Superdex 200 Increase column (GE Healthcare) in storage buffer (50 mM HEPES-NaOH ...
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bioRxiv - Genomics 2024Quote: ... and 100 µg of protein was purified using a 2D Clean-up Kit (GE HealthCare) following the manufacturer’s instructions with the exception that 400 μl of precipitant and co-precipitant were used in the first step ...
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bioRxiv - Immunology 2024Quote: ... staining or alternatively by immunoblotting transferring proteins to nitrocellulose membrane (GE Healthcare, Chicago, IL, USA) and detection using a primary anti-Penta-His antibody (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... antibodies in the supernatant were collected and captured by Magnetic Protein A-Sepharose (GE Healthcare). Bound antibodies were eluted by solution buffer Glycine pH 2.0 and further purified by gel filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Genetics 2024Quote: ... Proteins were detected by chemiluminescence using ECL SelectTM Western blotting Detection reagent (GE Healthcare, AmershamTM) and a Fujifilm LAS-3000 camera.
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bioRxiv - Neuroscience 2024Quote: ... The proteins were cleaved from the GST resin by incubation with PreScission Protease (GE Healthcare) overnight at 4°C.
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bioRxiv - Plant Biology 2024Quote: ... Biotinylated proteins were pulled-down using Streptavidin Mag SepharoseTM (Cytiva 28-9857-99; GE Healthcare) and then submitted to on-bead digestion ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were purified by GST affinity chromatography on the glutathione Sepharose beads (GE Healthcare) in lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 20 V for 60 minutes in transfer buffer (25 mM Tris base ...
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bioRxiv - Biophysics 2024Quote: ... The proteins were purified by affinity chromatography using an ÄKTA Start (GE Healthcare ÄKTA Start). The lysate was passed through a 5 mL pre-equilibrated HisTrap HP column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was then applied to a HiTrap S HP (5 ml) column (GE Healthcare) equilibrated in low salt buffer ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were purified by Ni-NTA (2 x HiTrap Chelating HP, 5 mL, GE Healthcare), buffer exchanged to cleavage buffer (20 mM TrisHCl pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... The chromatography was performed using a fast protein liquid chromatography system (Äkta go GE Healthcare) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) for 4h at 4°C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... Target proteins were initially purified by immobilized metal ion affinity chromatography (Chelating Sepharose, GE Healthcare) following a standard protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The GST-tagged proteins were purified using a glutathione Sepharose column (GSTrap HP, GE Healthcare), followed by overnight dialysis against A50 buffer containing 1 mM DTT at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and then the proteins were transferred onto the PVDF membrane (GE Healthcare Life Science, 10600023). The blocking and antibody incubation were performed accordingly ...
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bioRxiv - Cell Biology 2024Quote: ... Protein signal was visualized using the Amersham Imager 600 (GE Life Sciences #29-0834-61). The obtained images were quantified using Fiji (imagej.net/software/fiji/) ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Biophysics 2020Quote: ... the protein was loaded onto a HiLoadTM 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were purified by chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), in buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
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bioRxiv - Cell Biology 2020Quote: ... Further purification of S-nitrosylated proteins were done using Thiopropyl Sepharose 6B bead (GE Healthcare# 170420001) in the presence of 50mM Na-ascorbate as a mild reducing agent ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were visualised using Amersham™ Imager 600 (GE Healthcare Life Sciences, Malborough, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... and GST-tagged target protein was purified using Glutathione Sepharose 4B (GE Healthcare, Marlborough, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was subjected to size-exclusion chromatography on a HighLoad 16/60 Superdex75 (GE Healthcare).