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Citations for GE Life Sciences :
2351 - 2400 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the proteins were further purified on a HiLoad 16/600 Superdex 200 column (GE Healthcare). For purification of the GST-tagged proteins ...
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bioRxiv - Microbiology 2021Quote: ... Fc-fused nanobody was prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE using standard protocols for dimerization ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins was further purified using a 5 mL HiTrap HP Heparin column (GE Healthcare) and eluted with a buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was purified on a Ni2+-nitrilotriacetic acid (Ni-NTA) sepharose resin (GE Healthcare), eluting with a linear gradient of imidazole (100 – 400 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The protein of interest was purified from the medium on HisTrap™ Excel (GE Healthcare) as described above ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant of the soluble proteins was then loaded onto the HisTrap™ Excel (GE Healthcare) column in buffer A and eluted with a linear gradient of 0-100% of 1 M imidazole in buffer A in 20 column volumes (CV) ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein mass was loaded on SDS-PAGE and transferred to Hybond ECL (GE HealthCare) nitrocellulose membrane ...
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bioRxiv - Biophysics 2021Quote: ... Standard proteins used were from the gel filtration calibration kit LMW and HMW (GE Healthcare) with the exception of Cryptococcus neoformans acyl-CoA binding protein (ACBP ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Protein A Sepharose CL-4B and Glutathione Sepharose 4 Fast Flow were from GE Healthcare Life Sciences (Chicago ...
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bioRxiv - Cell Biology 2020Quote: ... Detection of the proteins was digitally acquired using an Amersham™ Imager 600 (GE Healthcare).
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Fate of the M-phase-assembled centrioles during the cell cycle in the TP53;PCNT;CEP215-deleted cellsbioRxiv - Cell Biology 2020Quote: ... Proteins at gels were transferred to Protran BA85 nitrocellulose membranes (10401196; GE Healthcare Life Sciences). The membranes were blocked with a blocking solution (5% nonfat milk in 0.1% Tween 20 in TBS or 5% bovine serum albumin in 0.1% Tween 20 in TBS ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized with an ECL western blotting detection kit (GE Healthcare, Chicago, IL, USA) through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin ...
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bioRxiv - Immunology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare) and cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare). The protein was cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with Protein G Sepharose 4 Fast Flow (GE Healthcare) overnight ...
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bioRxiv - Immunology 2021Quote: ... IgG3 antibodies were isolated using a HiTrap Protein G High Performance column (Cytiva, GE Healthcare). Antibodies were dialyzed overnight in PBS and filter-sterilized though 0.22 µm Spin-X filters ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein was separated with SDS-PAGE gels and transferred to PVDF membrane (GE Healthcare). Membranes were blocked with 5% BSA in TBST for 1h at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL, GE Healthcare). The full-length ACE2 construct contains the ACE2 protein (residues 1-805) ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated by 12% Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using an ECL Prime kit (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Biophysics 2022Quote: ... All proteins were further purified on a 5 ml HiTrap Heparin HP column (GE Healthcare), equilibrated in their respective dialysis buffer and eluted by an NaCl gradient containing 1 – 1.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The target proteins were purified with HisTrap HP columns (GE Life Sciences, Velizy-Villacoublay, France). Further purification was achieved by size-exclusion chromatography in buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing desired protein was applied to either Glutathione-Sepharose resin (GE Healthcare Life Science) for GST purification or Ni-NTA Agarose resin (QIAGEN ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved protein was further purified using a HiTrap HP Heparin column (GE Healthcare, Illinois, USA), eluting with a linear gradient to 1.0 M KCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
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bioRxiv - Plant Biology 2022Quote: ... protein bands and radioactive bands were detected with an ImageQuant LA4000 imaging system (GE Healthcare) and a Typhoon Trio phosphorimager (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... The intensity of the resultant protein bands was quantified using ImageQuant TL software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Protein was then purified from the supernatant using a StrepTrap HP column (GE Healthcare #28907547), followed by SEC purification over a S6 increase column (GE Healthcare #29091596 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified on a 5 ml HiTrap SP HP cation exchange column (GE Healthcare) and eluted with 1 M KCl ...
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bioRxiv - Neuroscience 2023Quote: ... Flag immunoprecipitates of IGF-1R-Flag were incubated with Protein G Sepharose beads (GE Healthcare). Beads were pelleted ...
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bioRxiv - Plant Biology 2022Quote: ... The protein was then enriched to high purity using a Ni-Sepharose column (GE Healthcare) and stored at 4°C before use.
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bioRxiv - Molecular Biology 2022Quote: ... the untagged protein was further purified through 15Q anion exchange column (GE Healthcare Life Sciences) and Uniondex 200 pg 16/60 size-exclusion column (Union Biotech ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were separated on a Superdex 75 10/300 increase SEC column (GE Healthcare) with a flow rate of 0.5 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... the Red1 proteins were polished using gel filtration (Superdex 75 16/60) column (GE Healthcare) equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were transferred using the semi-dry method onto a nitrocellulose membrane (Whatman, GE Healthcare). The membrane was blocked in the blocking buffer (5% skimmed milk ...
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bioRxiv - Immunology 2022Quote: ... The protein was fractionated by size-exclusion chromatography (Superose 6 increase 5/150, GE Healthcare) with buffer F (20 mM HEPES at pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were detected with a chemiluminescent substrate according to the manufacturer’s instructions (GE Healthcare). The membranes were processed for chemiluminescence detection of actin (43 kDa ...
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bioRxiv - Physiology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 100V for 90 min on ice in transfer buffer (48mM Tris–HCl and 39mM glycine supplemented with 20% methanol) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated on 7.5-12% SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Blots were blocked 1 h with 10% dry milk ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was further purified on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) in 10 mM Tris (pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm Amersham Protran® nitrocellulose membrane (GE Healthcare, Amersham) before being incubated in primary antibody resuspended in 5 % milk-powder at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... proteins were transferred to a 0.45-μm-pore-size nitrocellulose membrane (Hybond-ECL; GE Healthcare) and incubated with a α-HIS antibody (Genscript A00186) ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified by size-exclusion chromatography using a Superdex 200 column (GE healthcare) in SEC buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... Membrane total protein was visualized using an Amersham Imager 600 (AI600) (GE Healthcare Life Sciences) with UV transillumination to induce and capture a fluorescence signal ...
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bioRxiv - Biophysics 2023Quote: Proteins were resolved on 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (GE Healthcare). The primary antibodies used were mouse anti-EHD2 (L-05 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was further purified using a 5 mL HiTrap Heparin HP column (GE Healthcare), and then eluted with a linear gradient of 0.1-0.5 M NaCl ...
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bioRxiv - Microbiology 2023Quote: Protein samples were run on 12% polyacrylamide gels and transferred to nitrocellulose membranes (GE Healthcare) via semi-dry transfer in a Bio-Rad Trans-blot Turbo transfer machine ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred to previously hydrated with methanol PVDF membranes (Amersham™ Hybond, GE Healthcare) in a vertical tank (Mini-PROTEAN® Tetra Cell ...