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Citations for GE Life Sciences :
201 - 222 of 222 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Free dye was removed by size exclusion chromatography using a HiPrep 16/60 Sephacryl S 100 HR column (GE Healthcare) equilibrated with PBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The protein was separated from free dye using gel filtration on a Superdex 75 pg 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Labeling of Spt7 and residual dye depletion were confirmed by in-gel fluorescence imaging on a Typhoon imager (GE Healthcare). In vitro transcription activity of the nuclear extract was verified by the in vitro transcription assay described below.
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bioRxiv - Cell Biology 2020Quote: ... The labeled protein was buffer exchanged into 20 mM Tris pH 7.4 and 50 mM NaCl and unreacted dye was removed using HiTrap Desalting Columns (GE Life Sciences). Labeled protein was then snap frozen for storage at −80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Labeling of SNAP fusion proteins and depletion of free dye was confirmed by SDS-PAGE followed by fluorescence imaging on a Typhoon imager (GE Healthcare). Extracts were checked to verify transcription activity (e.g. ...
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Biochemistry 2020Quote: ... The reaction was quenched with 10 mM DTT and free dye was separated by size exclusion chromatography on a HiLoad Superdex 200 16/60 column (GE Healthcare). Fractions contained labelled protein were pooled and concentrated with Amicon Ultra-15 50 kDa cutoff centrifugal filter units (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: SpCas9hinge was either single labelled with Cy3 only or double labelled with Cy3 and Cy5 using maleimide monoreactive dyes (Amersham, GE Healthcare) (19) ...
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bioRxiv - Biochemistry 2022Quote: ... Removal of free dyes outside liposomes and exchange of outside buffer was achieved by passing samples through a desalting column (PD-10, GE Healthcare) equilibrated with the outside buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling of SNAPf fused activators and residual dye depletion were confirmed by in-gel fluorescence imaging on a Typhoon imager (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... Proteins in gels were either stained with Coomassie blue dye in gel or used for transfer onto nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in PBST (PBS and 0.05% Tween-20 ...
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... the excess unreacted dye was removed from each protein by passing it through a 600 μl Sephadex G-25 Fine resin (GE Healthcare), pre-equilibrated with a buffer containing 20 mM Hepes-K pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... dye-peptide-MHC complexes in PLB were purified using a 10,000–molecular weight cutoff spin concentrator (Vivaspin 500, GE Healthcare, Pittsburgh, PA) and 1x TBS wash [TBS ...
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bioRxiv - Biophysics 2021Quote: ... The suspension was incubated up to 1 h and the non-coupled free dye was removed by exclusion chromatography using a PD10 exclusion column (GE Healthcare, Sweden) equilibrated with 50 mM NaH2PO4/Na2HPO4 with 1% glycerol.
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bioRxiv - Cell Biology 2022Quote: ... 50 μg of protein per sample were solved in urea lysis buffer and labeled with 250 pmol CyDye DIGE Fluor dyes (GE Healthcare, Germany). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Cell Biology 2020Quote: ... Each oligonucleotide probe contained 4 primary amines that were conjugated to Cy3 using the Mono-Reactive Dye Pack (PA23001, GE Healthcare Life Sciences). To this end ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted fractions were concentrated and incubated with at least fivefold molar excess of Cy3- or Cy5-maleimide dye (GE Healthcare, Pittsburgh, PA) for 30 min at 25 °C and additionally for 14 h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... The labelled protein was purified from the excess of free dye by a P10 desalting column with Sephadex G25 matrix (GE Healthcare, Waukesha, WI, USA), divided into aliquots ...