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Citations for GE Life Sciences :
151 - 200 of 222 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... affinity columns of GST-fused Asct2(NT)-antigen and GST-fused Asct2(CT)-antigen were produced by conjugating the antigens with HiTrap NHS-activated HP (GE Healthcare). To purify anti-Asct2(NT ...
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bioRxiv - Biophysics 2020Quote: ... Free dyes were removed using a PD-10 desalting column (GE Healthcare Life Sciences). An additional IMAC was performed ...
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bioRxiv - Biophysics 2021Quote: ... Excess dyes were removed on a PDMiniTrap Sephadex G-25 desalting column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... Unbound dye was removed by PD-10 desalting column (GE Healthcare and Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Myelin was labeled using Amersham CyDye Cy5 Mono-Reactive Dye (GE Healthcare Life Sciences), using half of the recommended dose (1 vial per 2mg myelin) ...
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bioRxiv - Cell Biology 2024Quote: ... Unconjugated dye was removed by size exclusion chromatography (GE Healthcare, AKTA purifier, Superdex 200). IgG concentration was calculated as:
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Single-molecule FRET probes allosteric effects on protein-translocating pore loops of a AAA+ machinebioRxiv - Biophysics 2023Quote: ... Any unreacted dye molecules were removed on a desalting column (Sephadex G25, GE Healthcare). For the preparation of ClpB hexamers with only one labeled subunit ...
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bioRxiv - Microbiology 2021Quote: ... were isolated from buffy coats from healthy donors (UK NHS Blood and Transplant Service) by density centrifugation using FicollPaque Plus (GE Life Sciences) and cryopreserved in 10% DMSO (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Microbiology 2021Quote: mACE2 or hACE2 was immobilized onto a CM5 sensor chip surface using the NHS/EDC method to a level of ∼600 response units (RUs) using BIAcore® 3000 (GE Healthcare) and PBS as running buffer (supplemented with 0.05% Tween-20) ...
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bioRxiv - Biophysics 2023Quote: ... The amine modified dT nucleotides were labeled with Cy5 in a 20 µL reaction containing 10 mM (200 nmol) NHS-Cy5 (GE Healthcare, PA15100), 0.1 mM (2 nmol ...
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bioRxiv - Plant Biology 2024Quote: ... Enrichment of N-terminal labeled peptides was carried out using 200 µL NHS-Sepharose slurry (17-0906-01, GE Healthcare, Solingen, Germany) per 100 µg protein ...
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bioRxiv - Cell Biology 2021Quote: ... Excess dye was removed using a PD-10 desalting column (GE Healthcare, Chicago, IL, USA). Titrations were performed in PBS using a constant 1 μM concentration of fluorescein-coupled peptide with increasing concentration of R7R8 fragment (either wild type or vvv mutant) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was labelled individually with both Cy-3 and Cy-5 dyes (GE Healthcare) using Superscript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted dye was removed by passing through the PD MidiTrap G-25 column (GE Healthcare) three times ...
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bioRxiv - Biochemistry 2022Quote: ... EVs were labeled with a Cy3 Mono-Reactive dye pack (GE Healthcare Ltd., Tokyo, Japan) for detection by fluorescence microplate readers (Molecular Devices Japan Ltd. ...
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bioRxiv - Biophysics 2020Quote: ... followed by the separation of unreacted dye on a desalting column (Sephadex G25, GE Healthcare), buffer-exchange to native buffer (25 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... the dye values were measured using ImageQuant TL version 8 (GE Healthcare, Chicago, IL, USA) and Adobe Photoshop Elements were normalized to density values per mm2 of a worm’s projection area ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged RBD was concentrated to 5 mL and covalently coupled to a 5 mL HiTrap NHS-activated Sepharose column (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The unreacted dye was removed by gel filtration chromatography using Sephadex G-25 resin (GE Healthcare). For GrpE ...
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bioRxiv - Biophysics 2023Quote: ... Any unbound dye was subsequently removed using a HiPrep™ 26/10 Desalting column (GE Healthcare) connected to an ÄKTA™ FPLC system.
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bioRxiv - Biochemistry 2024Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2020Quote: We employed a protocol similar to that previously described.12 The broad-spectrum kinase inhibitor Ki-NET (CTx-0294885) was conjugated to NHS-activated Sepharose4 Fastflow resin (GE Healthcare, Little Chalfont, UK). Briefly ...
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bioRxiv - Cell Biology 2022Quote: White blood cells were isolated from leukocyte cones (NC24) supplied by the NHS blood and transplant service by centrifugation on Ficoll-Paque Premium (GE Healthcare 17-5442-03) for 60 min at 300 g ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Biophysics 2021Quote: ... separated from free dye by gel filtration using a PD-10 column (GE Life Sciences, Chicago, IL), and diluted to a working lipid concentration of 144 μM ...
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bioRxiv - Biophysics 2020Quote: ... The labeled viruses were purified from excess dyes on a Nap-5 gel filtration column (GE Healthcare) that was equilibrated with 50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... the modified proteins were purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
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bioRxiv - Biophysics 2020Quote: ... the free dyes were removed by centrifugation in MICROSPIN G-50 column (GE Healthcare, 27-5330-01). The labeled proteins were stored at −80°C ...
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bioRxiv - Biophysics 2021Quote: ... H2A.Z(K120C)-H2B-3xFlag and Swc7 were all cysteine-labeled with maleimide-functionalized Cy3 dye (GE Healthcare) using previously published protocols (53) ...
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bioRxiv - Microbiology 2022Quote: ... A488-Labelled phage was isolated from free dye by passing over two PD10 desalting columns (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... This protein was coupled to a 1 ml packed column volume of NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences, Marlborough, MA). 10 ml of serum was diluted 1:1 in binding buffer (20mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
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bioRxiv - Biophysics 2020Quote: ... excess FluoZin-1 dye was removed by passing the sample through a PD-10 desalting column (GE Healthcare) pre-equilibrated with reconstitution buffer.
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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bioRxiv - Biochemistry 2021Quote: ... The excess dye was separated from the labelled protein by SEC (Superdex 75 10/300 GL, GE Healthcare) performed in SEC buffer D (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... uncoupled dyes were separated from labelled antibodies by size exclusion chromatography (on Sephadex G25 PD10 columns GE Healthcare) followed by dialysis against PBS 1x overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fab were separated from unconjugated dye in an equilibrated PD MiniTrap G-25 desalting column (GE Healthcare). Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa ...
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bioRxiv - Biophysics 2023Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and buffer was exchanged for ultrapure water ...
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bioRxiv - Biophysics 2024Quote: ... The excess dye was removed using a PD-10 Sephadex G-25 desalting column (GE Healthcare, IL, USA), and the buffer was exchanged with ultrapure water ...
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Biochemistry 2021Quote: ... Free dye was removed from labelled MukB by SEC using a Superdex 200 10/300 GL column (GE Healthcare). Labelling efficiency (typically 40-70% ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the dye-labelled protein were detected by in-gel fluorescence imaging with an Amersham Typhoon (GE Healthcare) laser scanner.
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bioRxiv - Immunology 2024Quote: ... Unreacted dye was removed by size exclusion chromatography (SEC) using Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in HBS (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The protein dye mixture was then desalted in a pre-packed Sephadex G-25M column (PD-10, GE Healthcare) using labeling buffer ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... and in-gel fluorescence of the SecY-conjugated ATTO 643 dye was visualized (Amersham Imager 680RGB, GE Healthcare Life Sciences).