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Citations for GE Life Sciences :
1 - 50 of 222 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... an Amersham Cy3 mono-reactive NHS ester dye pack (GE healthcare) was resuspended with labeling buffer and mixed with enough purified APE1 to yield a 100 μL solution of 10 mg/mL APE1 ...
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bioRxiv - Biophysics 2022Quote: ... Lectins were labeled with Cy5 mono-reactive dye (NHS ester) from GE Healthcare. Briefly ...
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bioRxiv - Genomics 2023Quote: ... Cy3B–NHS ester (GE Healthcare PA63101), or Alexa Fluor 488–NHS ester (Invitrogen A20000 ...
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bioRxiv - Systems Biology 2024Quote: ... Cy3B–NHS ester (GE Healthcare PA63101), or Alexa Fluor 488– NHS ester (Invitrogen A20000 ...
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bioRxiv - Systems Biology 2020Quote: ... or Cy3B-NHS ester (GE Healthcare PA63101) or Alexa Fluor 488-NHS (Invitrogen A20000 ...
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bioRxiv - Systems Biology 2021Quote: ... or Cy3B-NHS ester (GE Healthcare PA63101) or Alexa Fluor 488-NHS ester (Invitrogen A20000 ...
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bioRxiv - Neuroscience 2024Quote: ... or Cy3B NHS ester (GE Healthcare, PA63101). GM1 was labeled with cholera toxin subunit B (CTxB ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP and Input samples were labeled with mono-reactive NHS ester fluorescent Cy5 and Cy3 dyes (GE Healthcare) respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling with Cy5-NHS ester (GE Healthcare, PA15101) yielded 54–70% labeling efficiency ...
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bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa-488 or Alexa-568 NHS esters (GE Healthcare) as described previously49.
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bioRxiv - Developmental Biology 2021Quote: ... with 4 µl of Cy5 NHS Ester (GE Healthcare; 1 mg dye dissolved in 10 µl of anhydrous DMSO ...
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bioRxiv - Biophysics 2022Quote: Cy3B-NHS ester (PA63101) was purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were labeled with Cy3 NHS ester (GE Healthcare) at an amine-modified internal thymine and HPLC purified on a 218TP C18 reverse-phase column (Grace/Vydac ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescently labeling of Pol β followed the same procedure using an Amersham Cy5 mono-reactive NHS ester dye pack (GE healthcare). Labeling efficiency was determined by calculating the molar ratio of dye-to-protein within the solution by measuring absorbance at 280 nm and 530 nm for APE1-Cy3 or 649 nm for Pol β-Cy5 ...
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bioRxiv - Cell Biology 2022Quote: For whole mount staining of embryonic hearts Ad2 was directly labelled via an NHS ester by the fluorescent dye Cy2 according to the instructions of the manufacturer (GE Healthcare, #32000). Ad2-Cy2 was applied at a concentration of 6 μg/ml in PBS/0.1%Tx100/5% goat serum for 2 hr at room temperature to paraformaldehyde fixed E10.5 hearts followed by several washing steps ...
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bioRxiv - Neuroscience 2020Quote: ... were coupled with Cy3B mono-reactive NHS ester (PA63101, GE Healthcare) according to the supplier’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Amersham Cy5.5 Mono NHS Ester was from GE Healthcare (Buckinghamshire, UK), Amicon Ultracel membranes (10 kDa ...
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bioRxiv - Microbiology 2022Quote: LecB was labeled with Cy3 mono-reactive NHS ester (GE Healthcare) or with biotin using NHS-PEG12-biotin (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... Cy3 or Cy5 NHS-dyes (GE Life Sciences) were dissolved in DMSO and added directly into the RNA-lysine solution mentioned above (final dye concentration ~ 500 μM from 10 mM stocks) ...
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bioRxiv - Biophysics 2023Quote: ... Cy3B NHS ester (Cat# PA63101) was purchased from GE Healthcare (Pittsburgh, PA). CK666 (Cat# ab141231 ...
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bioRxiv - Biophysics 2022Quote: Tubulin from bovine brain (PurSolutions) was labeled with Cy5 NHS ester (GE Healthcare) as previously described51.
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bioRxiv - Cell Biology 2022Quote: ... were pooled and coupled with either Cy®3 Mono NHS Ester (GE healthcare) or Alexa FluorTM (AF ...
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bioRxiv - Bioengineering 2024Quote: Tubulin from bovine brain (PurSolutions) was labeled with Alexa 568 NHS ester (GE Healthcare) following previously outlined procedures.
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bioRxiv - Bioengineering 2023Quote: Tubulin from bovine brain (PurSolutions) was also labeled with Alexa 568 NHS ester (GE Healthcare) as previously described(58).
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... an excess of Cy3b-NHS dye (50 μg, GE Life Sciences, # PA63101) was dissolved in DMSO and added to a solution containing 10nmol of the DNA ligand strand in 1xPBS with 0.1M NaHCO3 ...
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bioRxiv - Immunology 2020Quote: ... Cy3 conjugated to xfR5 mAb by Cy3 NHS Ester Mono-Reactive CyDye (GE Healthcare; PA, USA), following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... unbound Alexa Fluor 488 NHS Ester was removed with a PD-10 desalting column (GE Healthcare, Piscataway, NJ) using NH4OAc buffer (0.15 M ...
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bioRxiv - Neuroscience 2020Quote: ... Azido-PEG-11-alcohol was purchased from Broad Pharm and Cy5 NHS ester was purchased from GE healthcare and used as received ...
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bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Then cells were washed 3 times with PBS and stained with 75 μM CypHer5E NHS ester (GE Healthcare, PA #15401) for 1 hour at 25°C[13] ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... This oligo was conjugated with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1- carboxylate (SMCC linker through NH2-NHS ester reaction. The SMCC-linked oligo was purified through Nap column (GE Healthcare Life Sciences). The purified SMCC-linked oligo was conjugated with OVA through cysteine (thiol)-maleimide group coupling ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
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bioRxiv - Cell Biology 2023Quote: ... NHS-HiTrap (GE Healthcare, Ref. 17071701), HisPur™ Cobalt Resin (Thermofisher ...
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bioRxiv - Biophysics 2021Quote: ... 8 mM of NHS-Cy5 (GE Healthcare) or NHS-Sulfo-Cyanine 7 (Lumiprobe ...
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bioRxiv - Immunology 2020Quote: ... N-hydroxysuccinimide (NHS)-Sepharose beads (GE Healthcare) were crosslinked with recombinant proteins according to the manufacturer’s instructions and were used for the purification of antibodies against DJ-1 protein ...
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bioRxiv - Genomics 2022Quote: ... the DNA oligonucleotides conjugated with amino-ddUTP were mixed with 100 µg of Cy3-NHS or Cy5-NHS (Lumiprobe or GE Healthcare) in 0.1 M sodium bicarbonate and incubated overnight at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: NHS (N-hydroxysuccinimide) activated Sepharose 4 fastflow (GE Healthcare) beads coupled with an RNA aptamer alone control or aptamer fusion (zebrafish ndr1 3’UTR sequences fused to a Tobramycin aptamer ...
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bioRxiv - Microbiology 2020Quote: ... was covalently coupled to NHS HP SpinTrap (GE Healthcare) at 815 μg/mL of matrix ...
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bioRxiv - Molecular Biology 2022Quote: ... coupled to N-hydroxysuccinimidyl (NHS)-activated Sepharose (GE Healthcare) was used to immunocapture transferrin from plasma samples ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Cy3B-NHS (# PA63101) was purchased from GE Life Sciences. P2 gel (# 150-4118 was purchased from BIO-RAD ...
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bioRxiv - Biochemistry 2021Quote: ... Cy3 or Cy5 amine-reactive dyes (Amersham CyDye Mono-Reactive Dye Packs, GE Healthcare) were dissolved in 20 µL DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... Antigenic proteins were coupled to NHS-activated sepharose (GE Healthcare), whereas antigenic peptides were coupled to SulfoLink coupling gel (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP nanobody covalently coupled to NHS-agarose (GE Healthcare) and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NHS-activated Sepharose 4 Fast Flow was obtained from GE Healthcare and protein ladder from Thermo Fisher Scientific.
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bioRxiv - Molecular Biology 2021Quote: ... and affinity-purified using HiTrap NHS-activated HP columns (GE Healthcare) coupled with His-Dicer-2(1-257)-MBP ...
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bioRxiv - Cell Biology 2024Quote: NHS-Activated Sepharose 4 Fast Flow (GE Healthcare; Catalog number: 17090601)
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bioRxiv - Cell Biology 2024Quote: NHS-Activated Sepharose 4 Fast Flow (GE Healthcare; Catalog number: 17090601)