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Citations for GE Life Sciences :
2401 - 2450 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Total RNA of ARPE-19 cell lines was extracted using the Illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted and subjected to DNaseI treatment using the Illustra RNAspin kit (GE Healthcare). cDNA synthesis from the RNA samples was performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems #4374966 ...
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bioRxiv - Biochemistry 2022Quote: ... Bound secondary antibodies detected by chemiluminescence using the ECL kit according to the manufacturer’s instructions (GE Healthcare, Uppsala, Sweden). Finally ...
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bioRxiv - Immunology 2023Quote: ... Detection of HRP-linked antibodies was performed using chemiluminescence detection kits (ECL™ or ECL Plus™, GE Healthcare) according to the manufacturer’s instructions and visualized using ChemiDoc™ Touch Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2023Quote: The isolated protist cells were subjected to whole-genome amplification (WGA) using the Illustra GenomiPhi V2 Kit (GE Healthcare) as described previously [37] ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA extraction from bulk tumour or metastases samples was performed using the Illustra RNAspin Mini isolation kit (GE healthcare), following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was suspended in 50 µl of the hydration solution using a Tissue DNA isolation kit (GE HealthCare LifeSciences).
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bioRxiv - Synthetic Biology 2024Quote: ... cloned plasmids were verified by Sanger sequencing using template generated by the TempliPhi 500 Amplification Kit (GE Life Sciences) according to the manufacturers protocol or by Nanopore sequencing of purified plasmid DNA (Primordium Labs) ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was done using the chemiluminescent ECL-Plus detection kit (Amersham) on a digital Amersham Imager 680 (GE Healthcare). Semi-quantification of band intensity was determined using Image Lite Studio 5.2 software.
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bioRxiv - Genetics 2020Quote: ... Radiolabeled probes were prepared by random priming of PCR products covering the regions of interest with Megaprime kit (GE Healthcare) in the presence of α-32P dCTP (3000 Ci/mmol) ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were developed using the SuperSignal West Femto kit (Pierce) or fluorescence imaging according to the manufacturer’s protocol with Amersham Imager 600 (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Genomics 2020Quote: The total genomic DNA was extracted from a single aphid using Illustra Nucleon Phytopure kit modifying the manufacturer’s protocol (GE Healthcare). The lysis step was performed adding 10 ul of Proteinase K and incubating the sample in a water bath for 2 hours at 55°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was isolated from samples using illustra RNAspin Mini RNA Isolation Kit with RNase-free DNase I (GE Healthcare) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinants showing intense hybridization signal selected for the plasmid extraction using the IllustraTM plasmidPrep Mini Spin Kit (GE Healthcare). The inserts released by the EcoRI (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The ligated DNA templates were cleaned by an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, U.S.A). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Protein quantification was performed by gel densitometry measurements using the 1D analysis module of the ImageQuant TL software (v8.1) and the LMW-SDS Marker Kit (GE Healthcare) as standard ...
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bioRxiv - Zoology 2021Quote: ... Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare) as per manufacturer’s instructions (Figure A1 ...
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bioRxiv - Microbiology 2020Quote: ... Pure FasR and FasRF123A were dialysed against 10 mM sodium acetate pH 4.5 and coupled to a CM5 sensor chip using the Amine Coupling Kit (GE Healthcare). Micromolar concentrations of C20-CoA were dialysed against 25 mM Tris.HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... Digestion products were purified with the illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Little Chalfont, UK) and were directionally cloned in the yeast expression vector pYES2 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... and developed using the Amersham ECL Prime Western Blotting Detection Kit (catalog no. RPN2232; GE Healthcare Australia, Parramatta, NSW, Australia).
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bioRxiv - Microbiology 2021Quote: ... IgG secondary antibody for 1 h at RT and developed in Amersham ECL Western Blotting Detection Kit according to the manufacturer’s instructions (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... pH 4.0 were immobilized on a Biacore CM5 sensor chip (research grade) with the amine coupling kit following the manufacturer’s instructions (GE Healthcare). 1700 resonance units of FBN2-Ct or ADAMTS6-Ct was coupled to the chip for analysis in a Biacore 3000 instrument (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 2006 35 was covalently immobilized on a CM7 Series S sensor chip using an amine coupling reagent kit (GE Healthcare) at the range level of 3,000 to 5,000 response units (RU) ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... G2 and M phases of fertilized mouse eggs using the EllustraTM QuickPrep MicromRNA Purification Kit (GE healthcare UK Limited, UK). The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa) ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNAs were extracted from blood samples and amplified using illustra GenomiPhi V2 DNA Amplification kits (GE healthcare Life Sciences).
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bioRxiv - Microbiology 2023Quote: ... The positive DNA control was produced by random amplification of 2.5 µl DNA from the BAL of patient LA2 using the GenomiPhi HY kit (GE Healthcare), followed by a purification step using the columns of the QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: Phototransduction activity was assessed by measuring the light-induced hydrolysis of endogenous cyclic (c) GMP with an enzyme immunoassay kit (Biotrack EIA system) according to the manufacturer’s instructions (Amersham Bioscience GE Healthcare). Undifferentiated WTCAct or WTCUDC iPSC and cells differentiated into RS were kept in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were detected with a luminoimage analyzer (AE-6972C; ATTO, https://www.atto.co.jp) using Amersham ECL prime western blotting detection reagent kit (GE Healthcare, https://www.gehealthcare.com), as described previously (Rahman et al. ...
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bioRxiv - Microbiology 2024Quote: ... and amplified by Multiple Displacement Amplification (MDA) at 30°C for 2h using the Illustra GenomiPhi v3 kit (GE Healthcare). The SSU rRNA gene from each isolate was then amplified by PCR using the MDA product as template ...
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bioRxiv - Biochemistry 2024Quote: ... detection of the protein bands was achieved using either the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare, #RPN2232) or the Amersham™ ECL Select™ Western Blotting Detection Reagent (#RPN2235) ...
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bioRxiv - Plant Biology 2020Quote: ... Frozen tissue was ground in liquid nitrogen and total RNA was extracted using the GE Illustra RNA kit (GE Life Sciences), and 2 μg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Microbiology 2021Quote: ... All blots were washed 4X with PBS-T and then developed with a Amersham ECL Western Blotting Detection Kit (GE Healthcare).
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bioRxiv - Developmental Biology 2020Quote: ... 5 μg of total RNA was used to synthesize the first-strand cDNA using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer ...
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Biochemistry 2021Quote: ... The oligomerization state was determined by comparison of the elution volumes to the calibration curve of the column (Gel Filtration Calibration Kit, GE Healthcare). The fraction corresponding to the tetradecamer peak was analyzed by intact protein mass spectrometry ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were treated with peroxidase-conjugated secondary antibodies for 2h at room temperature and revealed using a chemiluminescence detection kit (ECL, GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... were loaded with 10 μL of Aβ42 oligomer samples alongside the Amersham High Molecular Weight calibration kit for native electrophoresis (GE Healthcare, USA). The gels were run at 4 °C using the electrophoresis system according to the Invitrogen instructions (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was neutralized by adding 1 M HCl and the cDNA was purified with the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). The 5’-3’ junction sequence was amplified by PCR with cox1 specific primers Atcox1-5’(−176..-196 ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Microbiology 2020Quote: ... The standard calibration curve was obtained using combined low molecular weight (LMW) and high molecular weight (HMW) Gel filtration Calibration kits (GE Healthcare). The resulting peaks from the elution of the protein were fractionated in 0.5 ml ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed three times with 1X TBST and processed with a LAS-4000 luminescent image analyzer (Fujifilm) after incubation with the ECL Prime enhanced chemiluminescence kit (GE Healthcare).
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bioRxiv - Evolutionary Biology 2021Quote: PCR products of the expected size (359 bp) yielding ≥ 20 ng were cleaned using ExoSAP-IT PCR Clean-up Kits (GE Healthcare). In cases where the yield of PCR products was lower than this threshold ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cleaned with an Illustra GFX PCR DNA and gel band purification kit (GE Healthcare, Chicago, IL, USA) and sequenced by using Sanger technology in an ABI 3730XL sequencer (STAB-VIDA ...
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bioRxiv - Biophysics 2022Quote: ... Protein concentration of the extracts were determined by SDS-PAGE and densitometry analysis using the LMW-SDS Marker Kit (GE Healthcare) as standard.
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...