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Citations for GE Life Sciences :
2151 - 2200 of 4368 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Supernatant containing desired protein was applied to Glutathione-Sepharose resin (GE Healthcare Life Science) equilibrated with lysis buffer and agitated for 2 hours ...
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bioRxiv - Physiology 2024Quote: ... Condensed protein was loaded onto Superdex 200 prep grade 10/30 column (GE Healthcare). The LBD samples following size exclusion chromatography was stored in 50 mM potassium chloride (pH 7.4) ...
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bioRxiv - Biophysics 2024Quote: ... The proteins were further purified by a Superdex 200 column (GE Healthcare Life Sciences) equilibrated with the balance buffer (50 mM PBS pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... The eluted proteins were gel filtered using a Superdex 75 Increase column (GE Healthcare) in storage buffer (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2024Quote: ... The GST-tagged proteins were extracted with Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 1.5-2 hr and recovered by 3C protease cleavage at 4°C overnight in 50mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was further purified by Superdex-75 size exclusion column (GE Healthcare LifeScience) in FPLC buffer (20 mM Tris-Hcl ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to an Amersham Hybond P 0.45 μm PVDF membrane (GE Healthcare). Membranes were blocked with 5% milk (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... and radiolabeled proteins were revealed with Typhoon™ FLA 9500 fluorescent scanner (GE Healthcare). The intensity of radioactive bands was quantified using the ImageQuantTL software (Molecular Dynamics ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... lysates were incubated with purified GST fusion proteins on glutathione-Sepharose beads (GE Healthcare), GFP-trap beads (Chromotek) ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein bands were detected using ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biochemistry 2024Quote: ... the proteins were polished using gel filtration (Superdex 200 16/60) columns (GE Healthcare) equilibrated with SEC buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified using a HiTrap SP HP cation-exchange column (GE Healthcare) with a linear gradient of 0–1000 mM NaCl in 50 mM sodium phosphate (pH 7.0) ...
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bioRxiv - Bioengineering 2024Quote: ... the proteins were purified using immobilized metal affinity chromatography using HisTrap HP (GE Healthcare), followed by gel filtration through HiLoad 16/60 Superdex 75 for Cre and Superdex 200 for the Cas9 fusion proteins ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was absorbed onto 1mL of Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 2 hrs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The proteins were loaded separately onto a size exclusion chromatography (Superdex S200, GE Healthcare) equilibrated with 20 mM Tris/HCl at pH 7.4 ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMC) were enriched by density gradient centrifugation of peripheral blood from healthy human donors through a Ficoll-Paque PLUS (GE Healthcare) gradient ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... 1 mM DTT) at 4°C overnight and then human cGAS was further purified on a PD-10 desalting column (GE Healthcare) eluted with storage buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... Human PBMCs were isolated from leukopheresis packs obtained from the Central Blood Bank (Pittsburgh, PA) via Ficoll-Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Microbiology 2021Quote: ... The primary antigen-antibody complex was detected by incubating the blot with goat anti-rabbit IgG HRP (1:10000, Jackson-ImmunoLab) and sheep anti-human IgG HRP (1:5000, GE Healthcare) in 3% milk in PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... lymphocytes and monocytes were isolated from heparinized human peripheral blood by Ficoll-PaqueTM PLUS (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) gradient centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... siRNA (Catalog no. L-013607-00-0005) Human DHX9 (1660) siRNA (Catalog no. L-009950-00-0005) were from Ge Healthcare Dharmacon (Colorado ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from human buffy coats from the New York Blood Center by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare). Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2023Quote: The complementary DNA of human ZIP4 (GenBank access number: BC062625) and human ZIP8 (GenBank access number: BC012125) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... was constructed by inserting the cDNA for human SSH1 (869–1049) into the BamHI and NotI sites of pGEX-4T (GE Healthcare). E ...
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bioRxiv - Microbiology 2024Quote: ... were isolated from patients with adult T-cell leukemia-lymphoma (ATL) and human T-cell leukemia virus type 1 (HTLV-1) carriers by Ficoll-Paque PLUS (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Microbiology 2020Quote: ... F(ab’)2 fragment was purified though HiTrap Protein A High Performance column (GE Healthcare) and HisTrap High Performance column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: ... The labelled protein was applied to a Thiopropyl Sepharose 6B column (GE Healthcare Life Sciences) to remove double labelled protein ...
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was then loaded onto a 5 mL HiTrap Heparin HP column (GE Healthcare), washed with 5 CV of Heparin Wash Buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The eluted protein was purified by Superose6 Increase 10/30 (GE Life Sciences, Cat. #29091596) gel filtration.
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bioRxiv - Biophysics 2020Quote: ... The protein solution was then loaded onto a Superdex 200 10/300 column (GE Healthcare). The peak fractions were pooled and concentrated (Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were passed over an either HisTrap or GstTrap FF 5□ml column (GE Healthcare), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Diluted proteins were subsequently passed over a HiTrap Heparin HP 5□ml column (GE Healthcare). After washing with binding buffer ...
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bioRxiv - Cell Biology 2020Quote: ... overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... HRP-labeled proteins were detected with Amersham ECL Western Blotting Detection Reagent (GE Healthcare, UK) or Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... along with 5µL of protein ladder (Full-Range RPN800E, GE Healthcare Rainbow Molecular Weight Markers). After transfer ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and proteins were electrotransferred to nitrocellulose membranes (Amersham Hybond-ECL, GE Healthcare, Chicago, IL, USA). The membranes were incubated overnight at 4°C with primary antibody (Table S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were loaded on a Superdex 200 16/60 column (GE Healthcare Life Sciences) equilibrated with 20 mM Tris-HCl pH 7.5 and 50 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was further purified using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated with 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Physiology 2021Quote: ... The protein solution was then dropped on a nitrocellulose membrane (GE Healthcare Protran® BA85) to form 2 μL and 5 μL dots for each concentration group ...
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bioRxiv - Developmental Biology 2020Quote: ... The fusion proteins were affinity-purified on Glutathione Sepharose® 4 Fast Flow (GE Healthcare), and then bound to POROS GoPure XS (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare). Then the RNAs were bound to the beads ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... Protein bands were visualized by chemiluminescence using the detection kit (GE Healthcare Europe, Solingen, Germany) on a chemiluminescence imager (INTAS Science imaging ...