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Citations for GE Life Sciences :
2301 - 2350 of 2500 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and purified using immobilized metal affinity chromatography via a HisTrapTM HP 5 mL (GE Healthcare, Chicago, IL, USA) and an AKTA FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... sfGFP-ACE2 was purified by an ion exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.) eluted by a linear salt gradient from 10 to 1000 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 5 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The samples loaded on SDS-PAGE and γ-32P phosphorylation signals were detected using Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2022Quote: ... Purification of resulting protein was performed by immobilized metal affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare, Frei-burg, Germany) containing HisTrap chelating stationary phase (Amersham Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was applied to an immobilized metal ion affinity chromatography column (HisTrap FF, 5 mL resin; GE Healthcare, Little Chalfont, UK). After washing with 25 mL of the 1 x T-buffer containing 25 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated pellet was resuspended in Buffer A and the solution subjected to Ni(II) immobilized affinity chromatography using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) with a gradient from 100% buffer A to 100% buffer B (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was exposed with phosphor imaging screens for 5 minutes before being scanned with a Typhoon™ FLA9500 (GE Healthcare Life Sciences). The bands corresponding to the probes were cut out of the gel and conserved at -20°C for future work ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants were applied to two connected Strep-Trap HP 5 ml columns packed with Strep-Tactin ligand immobilized in an agarose matrix (GE Healthcare Life Sciences) previously equilibrated with equilibration buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then vacuum filtered and loaded at 1 mL/min onto a HiTrap Q HP column (5 mL, GE Healthcare Life Sciences) pre-equilibrated with buffer D [10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred to nitrocellulose membranes and incubated with streptavidin-AlexaFluor-647 followed by fluorescence detection with a Cy5 filter (excitation/emission: 649/670 nm) with an Amersham Typhoon 5 Biomolecular Imager (GE Healthcare Life Sciences). FY01 contains a Cy5 fluorophore ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered (0.45 μm) and the target protein purified via immobilized metalion affinity chromatography (IMAC) using a 5 mL HisTrap™ HP column (GE Healthcare, UK). The lysis buffer (50 mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... The supernatant was then collected for purification using a 5 mL His Trap™ FF Crude Column containing Ni-NTA (GE Healthcare, USA). The column was first equilibrated using five volumes of His-tag Buffer + 10 mM imidazole and the supernatant was loaded into de column ...
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bioRxiv - Immunology 2021Quote: ... containing 5% non-fat milk (5% milk TBS-N) for 1 hour and probed with primary and HRP-conjugated secondary antibodies (GE Healthcare, 1:3000) diluted in 5% milk TBS-N for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed three times for 5 min in 0.05% PBST at RT and protein bands were subsequently visualized by ImageQuant™ LAS 4000 (GE Healthcare Life Sciences) using EZ-ECL Chemiluminescence detection kit for HRP (Biological Industries ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Analytical size exclusion HPLC of purified complexes of Af1:7B8 and Af2:7B8 were carried out using a Superdex 200 5/150 GL column (GE Healthcare, Freiburg, Germany) equilibrated in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed trice with 1× DPBS for 5 min each and then washed thrice for 20 min using HyPureTM molecular biology grade deionized distilled (DDI) water (GE Healthcare Life Sciences). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing the tagged domain were pooled and exchanged to the initial buffer containing 5 mM imidazole by using a PD10 desalting column (GE Healthcare Life Sciences). His-tagged TEV protease (0.1 mg/ml final concentration ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and C111S constructs were extracted from harvested cells by homogenization and soluble fractions loaded on a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) equilibrated in binding buffer [20 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Unencapsulated ANTS and DPX were removed by gel filtration through a 5 mL HiTrap Desalting Sephadex G-25 resin column (GE Healthcare Life Sciences). To ensure the correct size and obtain LUVs ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was filtered (0.2 µm) and applied onto a Ni-NTA column (HisTrap FF, 5 ml, GE Healthcare Europe GmbH, Freiburg, Germany). Bound protein was washed with 10 column volumes of washing buffer (25 mM TRIS-HCl pH 7.8 ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a 5 mL HiTrap Chelating HP Sepharose column on the ÄKTA FPLC protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with 10 mL of starting buffer A (native buffer + 10 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5 ml column, GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Physiology 2024Quote: ... and a multi-frequency linear-array probe (12 L-RS, 5 – 13 MHz, 38.4-mm field of view, GE Healthcare, Milwaukee, WI, USA) were used to obtain ultrasonography images of the dominant VL and RF (based on kicking preference ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted soluble fraction was then loaded at 1 mL/min in Buffer A onto a 5 mL HiTrap SPFF column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 70 mL gradient to 100% Buffer B ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered (0.45 μm filter) and loaded on a 5 mL HisTrap Fast Flow column (GE Healthcare, 17-5255-01). The purification was conducted on the bench using a peristaltic pump ...
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bioRxiv - Biophysics 2021Quote: ... the sample was concentrated to 2 mL and loaded onto a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare) equilibrated and run in Sizing Buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: Proteins (200 μl at 2 μM) were separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysed cells were centrifuged 20,000 xg for 30 minutes at 4C and soluble lysate was filtered prior to IMAC purification using 2×5mL His-Trap columns (GE Healthcare) affixed to an Akta Pure ...
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bioRxiv - Molecular Biology 2021Quote: ... The PAGE gel was exposed to a phosphosimage screen for ∼2 hours and analyzed using a Amersham Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the mixture was centrifuged for 2 mins and the supernatant was desalted by filtration with a Sephacryl S-400 HR column (GE Healthcare), centrifuged at 16 krpm ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for 1 hour prior to purification on a Superdex 200 10/300 size-exclusion column (GE Healthcare). Rac1 (G12V ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated to 2 mL and further purified by size-exclusion chromatography using HiLoad 16/600 Superdex 200 (Ge healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare) column into 20 mM NaPO4 150 mM NaCl pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... The ligase was inactivated with 2 M NaCl and the sample was injected into a Sephacryl S-1000 size exclusion column (GE Healthcare) to remove excess oligos and DNA ligase.
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bioRxiv - Bioengineering 2020Quote: Binding of SARS-CoV-2 RBD to ACE2 –Fc and ACE2mod–Fc fusion proteins were measured using a Biacore T200 instrument (GE Healthcare). Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU ...
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bioRxiv - Biochemistry 2021Quote: 50 μl of the N protein samples (1 mg/ml) purified according to protocols 1 or 2 were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with 20 mM HEPES ...