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Citations for GE Life Sciences :
2101 - 2150 of 2500 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The successfully cleaved samples were subsequently buffer exchanged using a Superdex S200 5/150 GL or 10/300 increase column (GE Healthcare) connected to an Agilent HPLC 1260 Affinity equipped with a fraction collector ...
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bioRxiv - Microbiology 2023Quote: ... After elution with 500 mM imidazole the VHH-containing fractions were buffer-exchanged with PBS using a Vivaspin column (5 kDa cutoff, GE Healthcare). The purified VHHs were analyzed by SDS-PAGE and Coomassie staining and by intact mass spectrometry ...
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bioRxiv - Biochemistry 2023Quote: ... Transcription template and HDV ribozyme with T7 promoter sequences were cloned into pUC57 vector and the extracted plasmids were further purified with Source Q 5/50 GL column (GE Healthcare). The DNA fractions were precipitated with 0.3 M NaAc and 70% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Capturing of ORF1p-His6 protein was performed using Immobilized-Metal Affinity Chromatography (IMAC) by loading of 7M Urea Extract on 5 mL HisTrap FF FPLC column (GE Healthcare) at 0.5 mL/minute flow rate followed by washing with 10 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... was performed at 20 °C using S75 (IDR constructs) and S200 Increase 5/150 (NTD and ARD constructs) analytical SEC columns (GE Healthcare) equilibrated in the appropriate buffers (see Tables S1 and S2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed in PBS-T and incubated with 1 mL of Enhanced ChemiLuminescence (ECL) (Amersham) reagent for 5 minutes to allow protein visualization on an x-ray Amersham Hyperfilm ECL (GE Healthcare). Fiji-Image J Software was used for quantification of the difference in protein expression comparing with GAPDH or HSP70 expression.
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Blots were developed with standard BCIP (5 % (w/v) in DMF) + 250 μL NBT (5 % (w/v) in 70 % DMF) or using the ECL western blotting reagent (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Immunology 2023Quote: ... 6 and 14 we euthanized 5 individuals per group and performed an intra-cardiac terminal blood collection for PBMCs isolation with Ficoll-Paque Plus (GE healthcare). We performed a complete necropsy of all animals and collected samples of the lungs ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h prior to exposure to a phosphor-screen for ∼5 h to overnight and imaged using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The fractions collected at the elution peak were pooled together and loaded into a MonoS 5/50 GL column (GE Healthcare) previously equilibrated with IEX Equilibration buffer (50 mM HEPES pH7.8 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions from ion-exchange chromatography were filtered through a 5 µm syringe filter and loaded onto a 22 mL Source 15ISO hydrophobic interaction column (GE Healthcare) equilibrated in buffer C (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Imidazole and NaCl present in the Ni-NTA running buffer were eliminated by using a HiTrap Desalting 5 mL (GE Healthcare) column.
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation with 20,000 x g followed by filtration with a 5 μM filter and loaded on a HisTrap HP column pre-equilibrated with lysis buffer (GE Healthcare). The column was washed first with lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was filtered and loaded onto a 5 mL HisTrap HP column (Cytiva) using an AKTA Start (GE Healthcare). The column was washed in buffer containing 50 mM Tris-HCl (pH 8) ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was treated with 100 mM phosphate buffer pH 7.5 and 50 mM EDTA before purification by gel filtration at room temperature on a Superose-6 Increase column (GE Healthcare) equilibrated with buffer containing 25 mM TRIS pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation with 20,000 x g at followed by filtration with a 5 μM filter before loading on a HisTrap HP column (GE Healthcare). The column was washed first with lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved sample was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences), and the flowthrough was collected ...
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bioRxiv - Biophysics 2024Quote: ... FcγRⅠ WT was diluted in acetic acid buffer pH 5 and immobilized on the surface of Series Sensor Chip CM5 (GE Healthcare) by amine coupling method ...
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bioRxiv - Biochemistry 2024Quote: ... at 8000 × g then subjected to two rounds of buffer exchange using equimolar Tris-KCl buffer and a 5-mL desalting column (HiTrap Desalting; GE Healthcare) pre-equilibrated with four column volumes of Tris-KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluent was combined with an additional 2x 20 mL of wash buffer and subsequently applied to a 5 mL HiTrap Q (GE Healthcare) ion-exchange column equilibrated in a buffer containing 25 mM Tris•Cl ...
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bioRxiv - Genomics 2024Quote: ... The mixed PCR fragments were digested with 5 restriction enzymes (AluI, RsaI, DdeI, HaeIII, and BfuCI) and subjected to labeling by incorporation of Cy3-dCTP (GE HealthCare) and Cy5-dCTP (GE HealthCare ...
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bioRxiv - Cell Biology 2024Quote: All TIRF experiments (except Figure 3 F and G) were acquired at 37 °C/5% CO2 with the DeltaVision OMX SR microscope (GE Healthcare) with a 60×/1.42 NA oil Plan Apochromat objective (Olympus ...
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bioRxiv - Microbiology 2024Quote: ... The cleavage solution was desalted using a HiPrepTM 26/10 Desalting column (Cytiva) before reverse affinity chromatography using the 5 ml Histrap column (GE Healthcare). Cleaved protein collected in the flow-through fraction was concentrated (30 kDa cut-off ...
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bioRxiv - Immunology 2024Quote: ... supernatants underwent protein affinity purification using a high-performance 5 mL HiTrap MabSelect SuRe column (Cytiva, catalog no. 11003494) from GE Healthcare Life Sciences for large-scale production ...
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bioRxiv - Neuroscience 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... Human monocyte cell line U937 was maintained at 37°C in 5% CO2 and RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Hyclone, GE Healthcare). Differentiation of U937 monocytes in macrophages was induced with 100 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Biochemistry 2024Quote: ... The proteases were then loaded onto an equilibrated MonoS 5/50 GL ion exchange column (GE Healthcare Cat. #17-5168-01) and washed with 10 column volumes (CV ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was cleared by centrifugation and loaded onto two serially connected HisTrap 5 ml columns (Cytiva) which were connected to a ÄKTA FPLC system (GE Healthcare). Columns were washed with lysis buffer and bound proteins were eluted with a linear imidazole gradient (20 – 500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... DsbA-RbmB was purified from the cleared lysate by loading the supernatant onto a cobalt-charged 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... Dried gels were then exposed to a storage phosphor screen for six days before acquiring radiograms using a Typhoon 5 Imager (GE Healthcare). All experiments were performed in three independent replicates that were carried out on three separate days ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered through a 0.45 µm membrane and loaded onto a nickel affinity column (5 mL GE Healthcare HisTrap). The protein was eluted using a gradient from 20 to 500 mM imidazole (solution B ...
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bioRxiv - Biophysics 2024Quote: ... The eluate was concentrated with 5 kDa spin concentrator and loaded on a Superdex 200 Increase 10/300 column (GE Healthcare) using 20 mM HEPES pH 7.5 ...