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Citations for GE Life Sciences :
2001 - 2050 of 3996 citations for 6 Quinoxalinecarboxylicacid 1 2 3 4 tetrahydro 2 oxo methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated with anti-LptD antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) at 4°C over-night with slow rotation ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 mg of trypsin digested peptides were separately processed on an AKTA Purifier UPLC system (GE Healthcare) fitted with an in-house column packed with 5-μm TiO2 beads (GL Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was nutated (2h, 4°C) with 10ml glutathione-Sepharose 4B resin (GE Healthcare, Pittsburg, PA) in resuspension buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
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bioRxiv - Biochemistry 2021Quote: Aβ42 monomers were immobilized by amine coupling onto flowcell 4 on a CM5 sensor chip (GE Healthcare) using a BIAcore 3000 instrument (BIAcore AB) ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were re-adsorbed onto a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was cleaved for 16 h at 4°C using 1U of thrombin (GE Healthcare) per 100 μg of total protein ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Plant Biology 2022Quote: ... crude extracts (4 ml) were passed through 0.5 ml DEAE-Sephadex A-25 columns (GE Healthcare, USA). The bound GSL was digested on the column for 18 h at room temperature with ∼0.8U myrosinase/thioglucosidase ...
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bioRxiv - Molecular Biology 2024Quote: ... Femurs were placed in a 4-limb positioning jig and scanned in eXplore Locus scanner (GE Healthcare). The femur containing jig was immersed in water and specimens were scanned at an 18 µm voxel size using the following settings ...
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bioRxiv - Microbiology 2023Quote: ... of which 450 µl were subjected to isoelectric focusing on pH 4-7 IPG strips (GE Healthcare), followed by SDS-PAGE with 12 % acrylamide gels ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Neuroscience 2023Quote: The binding of FUS to (G4C2)4 RNA was analyzed using a Biacore T200 instrument (GE Healthcare). (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K ...
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bioRxiv - Cell Biology 2024Quote: ... coli BL21 Star (DE3) and affinity-purified on a Glutathione Sepharose 4 Fast Flow column (GE Healthcare), followed by ion-exchange chromatography on a POROS GoPure HQ column (Applied Biosystems ...
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bioRxiv - Biochemistry 2019Quote: ... to 3 ml and the protein further purified by size exclusion chromatography (Hiload 16/600 Superdex 200 pg, GE healthcare) equilibrated with SEC buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Wet gels were exposed to a phosphor screen for 0.5 h to 3 h and then scanned using a Typhoon FLA 7000 imaging system (GE Healthcare), after which gels were stained with Coomassie Brilliant Blue.
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bioRxiv - Bioengineering 2020Quote: ... and 35 ml of this suspension was carefully added to 15 ml Ficol-Paque PLUS (1.077 g cm-3, GE Healthcare) and centrifuged at RT ...
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bioRxiv - Plant Biology 2021Quote: ... and the peptides used for SPR was dissolved in HBS-EP+ (10 mM HEPES, 150 mM NaCl, 3 mM EDTA and 0.05% [v/v] surfactant P20 (GE Healthcare)) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were separated into 24 fractions using the 24 cm IPG strips pH 3-10 NL (GE HealthCare, Chicago, IL). To achieve the required low salt concentration 2.5 mg wheat germ extract was diluted with water and centrifuged 10 min. ...
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bioRxiv - Biochemistry 2019Quote: ... Wet gels were exposed to phosphor screen (0.5–3 hrs) before being scanned using a Typhoon FLA 7000 imaging system (GE Healthcare). Background subtracted autoradiograph band intensities were determined with ImageStudio (Li-COR ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins into rehydration buffer were incubated with an 18 cm immobilized pH 3–11 nonlinear gradient dry strip (GE Healthcare) for 16 hours at 20°C ...
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bioRxiv - Immunology 2020Quote: ... with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences) size exclusion chromatography column previously equilibrated with 7M urea ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Physiology 2022Quote: ... Membranes were then washed 3 more times and the signal was detected by chemiluminescence using ECL Prime reagent (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... membranes were finally incubated for 3 min with an enhanced chemiluminescence assay (ECL-Kit, GE Healthcare Life Science, Amersham, UK) and digital images of the responsive bands automatically captured via an imaging system (ChemiDoc MP ...
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bioRxiv - Microbiology 2023Quote: ... and washed 10 minutes 3 times in PBST before being incubated with horseradish peroxidase-conjugated anti-rabbit IgG (GE Healthcare) diluted 1:2000 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or with horseradish peroxidase (HRP)-conjugated secondary antibodies followed by a 3-min incubation with a chemiluminescent substrate (GE Healthcare) for ChemiDoc imaging ...
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bioRxiv - Bioengineering 2023Quote: ... All images were acquired in a 3 Tesla MRI with 32 channel receiver system (GE Healthcare, Chicago, Illinois 60661, USA) and made anonymous in compliance with HIPAA standards and rules ...
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bioRxiv - Cell Biology 2022Quote: ... 6 µL of the reaction were diluted to 100 µL in 2x SSC (3 M NaCl, 0.3 M sodium citrate) and slot-blotted on a Hybond XL nylon membrane (GE Healthcare). For detection of ChIP samples ...
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bioRxiv - Neuroscience 2023Quote: The experiments were conducted using a 3-Tesla scanner equipped with an 8-ch phased array coil (GE Healthcare, Signa). Functional images were acquired using a T2*-weighted gradient-echo echo-planar imaging sequence ...
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bioRxiv - Neuroscience 2022Quote: ... the oligomers were separated from the monomeric and fibrillar forms of the protein by size exclusion chromatography on a Superose 6 HR10/300 column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA) equilibrated in PBS pH 7.4 at a flow rate of 0.5 mL/min ...
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bioRxiv - Immunology 2022Quote: ... The cell free supernatant derived during harvest of the fermented broth was processed using Blue Sepharose™ 6 Fast Flow column (GE Healthcare, Cat# 17-0948-01) in flow-through mode followed by a hydrophobic interaction chromatography (HIC ...
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bioRxiv - Cell Biology 2021Quote: ... Bound proteins were then eluted with 100 mM L-glutathione reduced and subjected to a second purification by addition of Nickel-Sepharose beads 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA). The protein was eluted with HKI500 buffer (20 mM Hepes ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of a Protein A sepharose (nProtein A Sepharose 4 fast flow, GE Healthcare, 17-5280-01) suspension was added and incubated for 1 hour at 4°C on a rotator ...
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...