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Citations for GE Life Sciences :
1801 - 1850 of 3996 citations for 6 Quinoxalinecarboxylicacid 1 2 3 4 tetrahydro 2 oxo methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 4°C and loaded on a HisTrap column (GE Healthcare, Chicago, Illinois, United States) for purification ...
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bioRxiv - Molecular Biology 2019Quote: ... chromatin lysates were incubated for 4 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... 4°C and applied to a HiLoad 16/600 Superdex 200 column (GE healthcare) at 1 ml/min ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Biochemistry 2019Quote: Size-exclusion chromatography was performed at 4 °C on an AKTA FPLC (GE Healthcare). Approximately 10 mg of apoferritin (Sigma A3660-1VL ...
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bioRxiv - Biochemistry 2020Quote: ... 30μl of beads coupled with ProteinA or ProteinG (GE Healthcare 4 Fast Flow Sepharose), previously blocked with 0.5mg/ml chicken egg albumin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was mixed with 4 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 and suspension was applied on Flex-Column (420400-2510 ...
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bioRxiv - Neuroscience 2020Quote: ... Scans were performed using a 4-10 MHz endocavitary transducer (GE Healthcare, Milwaukee, WI). Patients were in lithotomy position with empty bladder ...
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bioRxiv - Cell Biology 2021Quote: ... 3D-SIM was performed using a DeltaVision OMX Version 4 Blaze microscope (GE Healthcare), equipped with 405- ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Protein A Sepharose™ 4 Fast Flow sepharose beads were purchased from GE Healthcare Life Sciences (Pittsburgh ...
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bioRxiv - Cancer Biology 2022Quote: ... for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare, SH30027.01) supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... 50 μl of pre-washed protein-G sepharose-4 fast flow (GE Life Sciences) was added to each tube and tumbled end-over-end for 4 h at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to a GFP nanobody expressed and purified by published protocols45 and incubated on a rocker for 1.5 hours ...
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bioRxiv - Microbiology 2019Quote: ... coli expressed SDAg6 to CNBr-Activated Sepharose 4 Fast Flow (GE Healthcare Life Sciences) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... MAbs were purified over Protein G Sepharose 4 Fast Flow resin (GE Healthcare; 17061802). The antibodies were buffer exchanged into PBS pH 7.4 prior to use in any cell based assays.
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was incubated with CNBr-Activated Sepharose 4 Fast Flow beads (GE Healthcare) coupled with an anti-GFP nanobody39 resin for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified from lysates with glutathione-Sepharose 4 Beads (GE Healthcare). His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for rabbit antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... were used in association with nPprotein A Sepharose 4 Fast Flow beads (GE Healthcare) whereas 10 μg of anti-Sir2 antibody (Santa Cruz ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... and cleaved from GST resin overnight at 4°C with PreScission Protease (GE Healthcare). GST was affinity purified as described above and eluted using 15 mM reduced L-gluathione ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Genetics 2024Quote: ... which were conducted in the presence of Protein G Sepharose 4 beads (GE Healthcare). For phospho-CTD ChIP ...
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bioRxiv - Neuroscience 2023Quote: ... with MOPS buffer and transferred overnight at 4°C onto nitrocellulose membranes (GE Healthcare) with Tris-Glycine transfer buffer (25 mM Tris-base ...
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bioRxiv - Immunology 2023Quote: ... or polyclonal IgG affinity-purified with Protein G Sepharose 4 Fast Flow (GE Healthcare) from heat-inactivated and filtered plasma were mixed with 10 TCID50 of SIVmac239 at a 1:1 ratio (5 μl:5 μl ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to Protein G Sepharose 4 Fast Flow beads (GE Healthcare) preincubated with or without MLCK mouse monoclonal antibody (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysate was then loaded onto glutathione SepharoseTM 4 Fast Flow resin (GE Healthcare), and washed with Tris buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting sera were purified with CNBr-activated sepharose 4 Fast Flow (GE Healthcare) conjugated with the same peptide ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated overnight at 4 °C with anti-GST antibody (GE Healthcare) at a 1/1000 dilution ...
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bioRxiv - Biophysics 2024Quote: ... Supernatant was mixed with GFP nanobody-conjugated Sepharose 4 Fast Flow resin (GE Healthcare). Resin was washed with buffer containing 0.06% digitonin ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) coupled to an anti-GFP nanobody expressed and purified by published protocols2 and incubated on a rocker for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... CNBr-Activated Sepharose 4 Fast Flow was obtained from GE Healthcare (17-0981-01). NanoGlo lytic detecton system was obtained from Promega (N3030) ...
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bioRxiv - Microbiology 2020Quote: ... S protein was purified by running the supernatant through a streptactin column and then by size exclusion chromatography using a Superose 6 increase 10/300 column (GE Healthcare Biosciences). Protein fractions corresponding to the trimeric S protein were collected and concentrated.
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bioRxiv - Cell Biology 2020Quote: ... Elutions with CAV1 were concentrated and further purified by size exclusion chromatography using Superpose 6 Increase 10/300 GL (GE Healthcare, Illinois) equilibrated with a buffer composed of 200 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... BG505 Env trimer was purified from transfected cell supernatants as described47 by 2G12 immunoaffinity and size-exclusion chromatography (SEC) with a Superose 6 16/600 column followed by Superdex 200 Increase 10/300 GL column (GE Life Sciences).
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL or Superose 6 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: Size exclusion chromatography experiments with HeLa cell extracts were performed with a pre-packed Superose 6 10/300 GL column (GE Healthcare ©). The column was equilibrated with buffer A (20 mM Hepes ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 10/300 column or on a Superose 6 5/150 column (GE Healthcare, Germany) in gel filtration buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy and visualized by SDS-PAGE with silver staining (ProteoSilver™ Plus Silver Stain Kit) ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved topo VI fractions were pooled and concentrated using Amicon Ultra 15 mL centrifugal filter units (30 kDa cut off, MilliporeSigma) before being passed down a Superose 6 10/300 (GE Life Sciences) column in Buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy ...
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bioRxiv - Biochemistry 2019Quote: ... the reaction was quenched with 10 mM DTT and subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 (GE Healthcare) in GF buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The reaction was stopped with 1.7 mM Tris-HCl pH 8.0 and crosslinked TcdA1-BSA-Lewis X was purified from excess of BSA-Lewis X by SEC using a Superose 6 3.2-300 column (GE Healthcare Life Sciences).
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bioRxiv - Genomics 2021Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Complex samples were either diluted to 0.02 mg/mL and applied to glow discharged negative stain grids or they were SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column to remove excess Fab prior to EM grid preparation ...
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bioRxiv - Genetics 2020Quote: ... the eluted protein solution was concentrated 10 times in a Vivaspin concentrator 30 kDa cut off and loaded in a Superose 6 increase 10/300 (GE Life Sciences) with PBS as a carrier buffer ...