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Citations for GE Life Sciences :
1901 - 1950 of 3996 citations for 6 Quinoxalinecarboxylicacid 1 2 3 4 tetrahydro 2 oxo methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Biophysics 2020Quote: ... The flow-through was then collected and concentrated using a 100 kDa concentrator and further purified on a Superose 6 gel filtration column (GE Healthcare Life Sciences) in 20 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: Protein extracts (150 mg per sample) were incubated with 300 µl of IgG-Sepharose 6 Fast Flow beads (GE Healthcare, Chicago, IL, USA) for 2 h at 4°C with gentle rotation (1000 rpm) ...
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bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was injected into a Ni2+-NTA agarose affinity column (containing 5 mL Ni2+-Sepharose 6 FF beads, GE Healthcare, Cat. 17531803). The column was incubated for 15 minutes and washed twice with 30 mL washing buffer (50 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... This was performed using an purifier system connected to a Superose 6 Increase 10/300 GL column (both from GE healthcare, Solingen, Germany) at a flow rate of 0.5 ml · min−1 in running buffer (50 mM Tris/HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and then incubated with 500-μl of Nickel-Sepharose 6 Fast Flow (GE Healthcare Life Sciences, Piscataway, NJ, USA) for 2 h at 4°C ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Mpro was eluted with 300 mM imidazole in the lysis buffer and then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size- exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s protocol and removed the FLAG tag and His6-tag with TEV digestion followed by reverse nickel affinity purification and sizeexclusion chromatography on a Superose 6 Increase column (GE Healthcare Life Sciences). We biotinylated the protein with BirA ligase as previously described32.
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bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
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bioRxiv - Microbiology 2020Quote: ... The sdAbs with a His-tag fused at C-terminus were purified over Ni Sepharose 6 Fast Flow (GE Healthcare, Boston, MA, USA) and eluted with 400 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... were mixed together and incubated for at least 1 h at 4 °C before removing the excess of free TcB-TcC by SEC on a Superose 6 Increase 5-150 column (GE Healthcare Life Sciences). For TcB-TcC variants containing HVR-RBD chimeras ...
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bioRxiv - Immunology 2020Quote: Purified C105 Fab was incubated with SARS-CoV-2 S trimer at a 2:1 molar ratio per protomer on ice for 30 minutes prior to purification by SEC on a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences). Fab–S complexes were concentrated to 1.6 mg/ml in Tris-buffered saline (TBS) ...
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bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
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bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The digested protein was concentrated using a 100 kDa concentrator and further purified on a Superose 6 Increase 10/300 gel filtration column (GE Healthcare Life Sciences) equilibrated in an Mg2+-containing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free biotin (or dye) was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labelled protein on a Superose 6 gel filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Eluted fractions were diluted 3-fold with R buffer and loaded onto a HiTrap SP column (GE Healthcare). Rrp1 eluted at around 600 mM NaCl with a linear gradient of 0.1-1.0 M NaCl in R buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was transferred on a column containing 3 ml Ni-Sepharose High Performance resin (GE Healthcare), washed two times with lysis buffer containing 30 mM and 50 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... zGDNFmat -zGFRα1D1-3 was further purified using size exclusion chromatography using a Superdex 200 (16/600) (GE Healthcare) in 20 mM Tris (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... separated by SDS-PAGE using a 3-15% Tris-Acetate gel and transferred to nitrocellulose membranes (GE Healthcare). Densitometry analysis was performed by the Image J software (National Institutes of Health).
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bioRxiv - Neuroscience 2020Quote: ... age range: 20-29 years) were scanned in a 3-Tesla MRI scanner (DISCOVERY MR750, GE Healthcare, USA) using a 32-channel head-coil ...
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bioRxiv - Immunology 2020Quote: Whole-blood leukocyte DNA was extracted using the Nucleon BACC 3 DNA extraction kit (GE healthcare, Buckinghamshire, UK). We genotyped patients using the Illumina OmniExpressExome-8 Bead Chip (Illumina ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Membranes were washed with PBS-T for 3 × 10 min and were developed by ECL Prime (GE Healthcare) and imaged on a Biorad ChemiDoc MP Imaging System.
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bioRxiv - Neuroscience 2023Quote: ... MRI images were acquired with the aid of a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI) and an 8-channel head coil ...
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bioRxiv - Immunology 2024Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Biochemistry 2019Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tagged proteins were filtered on a Superdex 200 10/300 GL or Superose 6 Increase 10/300 GL column (GE Healthcare, Little Chalfont, UK) and then labeled with SNAP-surface-549 (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified extract was either directly loaded onto Superose 6 column following pre-clearing with 150 μl of Sepharose CL-2B (GE Healthcare, 17-0140-01) or subjected to Halo purification by incubating with 100 μl of HaloLink™ Resin (Promega ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
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bioRxiv - Biophysics 2022Quote: ... The caveolin-containing eluate was concentrated and further separated by size exclusion chromatography using a Superpose 6 Increase 10/300 GL column (GE Healthcare, Boston, MA, USA) in buffer containing 200 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation procedures were performed by mixing ~1.6 mg of total proteins and 30 μl of the IgG Sepharose 6 Fast Flow beads (GE Healthcare, Cat# 17-0969-01) at 4°C for 2h and precipitating the NTAP tagged Orc4 ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-AtEPL1A and GST-AtEPL1B were purified on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Biochemistry 2019Quote: ... Protein A Sepharose 4 Fast Flow and Con A Sepharose 4B were purchased from GE Healthcare.
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was carefully layered on top of 4 ml of 90% Percoll (GE Healthcare, Chicago ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were incubated with appropriate antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) or Ni-NTA Agarose (QIAGEN ...
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bioRxiv - Cell Biology 2021Quote: ... Image stacks spanning full ICDs were acquired using a DeltaVision OMX-Blaze (Version 4; Applied Precision) equipped with a 60x PL APO NA = 1.42 objective (Olympus ...
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bioRxiv - Plant Biology 2020Quote: Experiments were conducted at 4°C using a Superdex 75 10/300 GL column (GE Healthcare) equilibrated in running buffer (20 mM HEPES pH 7.5 ...