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Citations for GE Life Sciences :
151 - 200 of 4182 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... designs (labeled and unlabeled) were further purified by size-exclusion chromatography on ÄKTAxpress (GE Healthcare) using a Superdex 75 10/300 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... probed with a specific 32P-labeled probe and scanned with a Typhoon Imager (GE healthcare). In JRL92 a CAN1 fragment was used as a probe ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled RNA substrates were subsequently separated from unincorporated γ32P using Microspin G25 columns (GE Healthcare) or ethanol precipitation ...
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bioRxiv - Neuroscience 2022Quote: ... and labeled with pCd-Cy3 using T4 RNA ligase (GE Healthcare Europe GmbH, Glattbrugg, Switzerland). The labeled RNAs were hybridized to a Mouse miRNA Microarray (G4472B ...
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bioRxiv - Biophysics 2023Quote: ... The labeled BRICHOS was separated from unreacted label by a PD-10 column (GE Healthcare) equilibrated with PBS ...
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bioRxiv - Bioengineering 2023Quote: Tubulin from bovine brain (PurSolutions) was also labeled with Alexa 568 NHS ester (GE Healthcare) as previously described(58).
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bioRxiv - Pathology 2024Quote: ... Chylomicrons were labeled with Cy5 according to manufactures instructions (GE Healthcare UK limited, Buckinghamshire, UK) and injected into hepatectomized rabbits (n=3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified using HiTrap protein A column (GE Healthcare, 17040303).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTA micro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were purified from excess SNAP-biotin by size-exclusion chromatography on a Superdex 75 Increase 3.2/300 column using an ÅKTAmicro system (GE Healthcare) in gel filtration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Capture of biotin-tagged nascent DNA was performed by incubating samples with streptavidin sepharose high performance (GE Healthcare Life Sciences) agarose beads for 16 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then hybridized with 32P-labeled oligonucleotide probes using the Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to PhosphorImager screens and signals were revealed using Typhoon imager (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... α [32P] dCTP-labeled probes were generated by random priming (Megaprime DNA labeling kit; GE Healthcare). Hybridization was performed in Church Buffer (1% [w/v] BSA ...
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bioRxiv - Cell Biology 2021Quote: ... The fluorescently labeled membrane was imaged by the Typhoon FLA 9500 scanner (GE Healthcare Life Sciences) per the manufacturer’s protocol.
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bioRxiv - Biophysics 2020Quote: ... 6-FAM labeled RNA products were visualized by Typhoon 95000 FLA Imager (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2020Quote: ... The washed membrane was incubated with a secondary antibody labeled with horseradish peroxidase (HRP) (GE healthcare) for 1 hour at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The fluorescently-labeled mAb conjugates were purified via size exclusion chromatography (PD-10 column; GE Healthcare) and concentrated using 50 KDa cutoff amicon filters.
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at room temperature for 1 hour prior to removal of unreacted Biotin-NHS by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Biophysics 2020Quote: ... 3’ biotinylated RNA constructs (linked via an extended TEG spacer arm) were purchased from Integrated DNA technologies (Baulkham Hills, NSW) and immobilised on a Biotin CAPture chip (GE Healthcare) in 10 mM sodium acetate pH 4.8 ...
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated in horseradish peroxidase-labeled goat anti-rabbit or goat anti-mouse antibodies (GE Healthcare; 1:10,000) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Labeled probes were cleaned up with MicoSpin S-200 HR columns (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual.
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Biophysics 2020Quote: ... The labeled viruses were purified from excess dyes on a Nap-5 gel filtration column (GE Healthcare) that was equilibrated with 50 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The dual-labeled heterodimer was then purified by cation exchange chromatography (Mono S 10/100, GE Healthcare) in 25 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Excess P32 gamma-ATP was then separated from labeled AAF DNA by G50 spin column (GE Healthcare). Labeled AAF DNA was mixed 1:4 with unlabeled AAF DNA prior to use for electromobility shift assays (EMSA).
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bioRxiv - Molecular Biology 2019Quote: ... was labeled with dCTP α-32P using Amersham Rediprime II DNA Labelling kit (GE Healthcare, Buckinghamshire, England) and used for hybridization at 42 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit; GE Healthcare). Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... H2A.Z(K120C)-H2B-3xFlag and Swc7 were all cysteine-labeled with maleimide-functionalized Cy3 dye (GE Healthcare) using previously published protocols (53) ...
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bioRxiv - Biochemistry 2022Quote: ... Radioactive labeled mitochondrial translation products were detected using Phosphor screens and Amersham Typhoon imaging system (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... a probe was labeled using the Amersham Gene Images AlkPhos Direct Labelling and Detection System (GE Healthcare) according to the manufacturer’s protocol with a DNA fragment generated by PCR using primer pair S01/S02 (Supp ...
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bioRxiv - Microbiology 2023Quote: ... Cy5-labeled cDNAs were detected by fluorescent mode using a Typhoon FLA 9500 (GE Healthcare Life Sciences) Gel Scanner and LPR Ch.2 filter and 635 nm laser.
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bioRxiv - Biochemistry 2023Quote: ... Radio-labeled analytical gels were dried onto Whatman paper and exposed to Storage Phospho Screens (GE Healthcare). The phosphoscreens were scanned with the Typhoon Trio+ with an excitation wavelength of 633 nm and an emission filter of 390 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled probes were then purified trough a G50 column from the Illustra MicroSpin G50 kit (GE Healthcare) and precipitated overnight with Salmon sperm DNA (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Protein purification was carried out with the AKTA-prime protein purification system (GE Healthcare). First ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from supernatants by protein A affinity chromatography using ÄKTA (GE Healthcare) or NGC (Bio-Rad ...