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Citations for GE Life Sciences :
1 - 50 of 4182 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Myelin was labeled using Amersham CyDye Cy5 Mono-Reactive Dye (GE Healthcare Life Sciences), using half of the recommended dose (1 vial per 2mg myelin) ...
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bioRxiv - Biochemistry 2019Quote: ... resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare) in 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Free biotin was removed using size-exclusion chromatography by loading the labeled protein on a Superose 6 gel-filtration column (GE Healthcare, Pittsburgh, PA) eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... Biotin-labeled SUMO-fusion TRIM14 was coupled on the sensor chip SA (GE Healthcare). Dilution series of TBK1 or IRF-3 (1.25 ...
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bioRxiv - Biochemistry 2023Quote: ... Labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with the storage buffers of proteins (hSSB1 ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Biochemistry 2019Quote: ... The labeled proteins were purified using a PD10 (GE Healthcare) gel filtration column ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled proteins were purified with a PD10 (GE Healthcare) gel filtration column pre- equilibrated with Storage buffers (hSSB1 ...
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bioRxiv - Biochemistry 2024Quote: ... The labeled protein was purified with a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer and dialyzed against storage buffer to remove residual free dye.
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bioRxiv - Biochemistry 2024Quote: ... the labeled protein was purified using a PD10 (GE Healthcare) gel filtration column pre- equilibrated with INIP Storage buffer (25 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated proteins were immobilized on a CAP chip (Biotin CAPture kit, GE Healthcare). As a blocking control anti-LILRB2 antibody ...
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bioRxiv - Microbiology 2022Quote: SEC analysis of single proteins or combinations of the subunits was conducted using AKTA basic 10 FPLC system (GE healthcare) and a Superdex 200 Increase 10/300 SEC column ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Microbiology 2020Quote: ... Biotin CAPture Reagent provided in the Biotin CAPture Kit (GE Healthcare) was captured onto the sensor chip according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Mononuclear cells were separated from myelin via centrifugation in a 27% Percoll solution (GE Healthcare).
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bioRxiv - Neuroscience 2022Quote: ... Further astrocyte enrichment was accomplished by removing myelin with PercollTM (GE Healthcare, GE17-0891-02) gradients ...
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bioRxiv - Biochemistry 2023Quote: ... The labeled proteins were purified with size-exclusion chromatography (Superdex 200 column, GE Healthcare) with a buffer containing 50□mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-labeled proteins were detected with Amersham ECL Western Blotting Detection Reagent (GE Healthcare, UK) or Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescence-labeled protein was visualized with a Typhoon trio fluorescence scanner (GE Healthcare, Chicago, IL) and relative protein quantities were determined using FIJI (Schindelin et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were separated from myelin and debris by 30% isotonic percoll gradient (#17-0891-01, GE Healthcare) in Myelin Gradient Buffer (MGB) ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was removed by resuspending pelleted cells into 30% isotonic Percoll (GE Healthcare, Cat#17-5445-02) and centrifuging at 300g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The labeled proteins were further purified using the Superdex 200 10/300 columns (GE Healthcare, USA). As for TDP-43 LCD ...
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bioRxiv - Biochemistry 2023Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Biophysics 2023Quote: ... Single labeled protein was isolated via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare - protein bound in buffer A (+5 mM βME) and eluted with 0-40% buffer B (buffer A + 1 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Labeled protein was further purified on a Superdex 200 increase 10/300 gel filtration column (GE Healthcare) equilibrated in buffer B + 100 mM potassium acetate (KOAc) ...
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bioRxiv - Microbiology 2019Quote: Fifty micrograms of protein extract from each sample were labeled with Cy3- or Cy5 Dye (GE Healthcare). Labeling was performed reciprocally so that each sample was separately labeled with Cy3 and Cy5 to account for any preferential protein labeling by the CyDyes ...
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bioRxiv - Cell Biology 2021Quote: ... Labeled proteins were purified by final buffer exchange using size exclusion chromatography (Superdex 200 column, GE Healthcare) in the appropriate buffer (i.e ...
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bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was loaded using an ÄKTA Basic HPLC (GE Healthcare) system onto an XK 16/20 column packed with Q-Sepharose HP ion-exchange media (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The biotinylated protein was separated from excess biotin on a Superdex 200 Increase 10/300 gel filtration column (GE Healthcare) equilibrated in 1x PBS buffer (10 mM Na2HPO4·7H2O ...
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bioRxiv - Cell Biology 2020Quote: ... the labeled protein was separated from the solution by size exclusion chromatography using PD MiniTrap G25 (GE Healthcare). PLCγ1 nSH2-cSH2-SH3 and truncations were labeled with CoA 647 (NEB ...
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bioRxiv - Biophysics 2022Quote: ... Labeled proteins were separated from free dye molecules by gel filtration using a Superdex 200 column (GE Healthcare)56.
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bioRxiv - Biochemistry 2022Quote: ... the labeled KRAS protein was purified by size-exclusion chromatography using 16/60 Superdex75 increase column (GE Healthcare) pre-equilibrated with 20 mM HEPES (pH 7.0) ...
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bioRxiv - Molecular Biology 2019Quote: ... The absorbance of fluorescently labeled proteins was followed during chromatography using the AKTAmicro Monitor UV-900 (GE Healthcare) and by SDS-PAGE using a ChemiDoc MP system (Bio-Rad).
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Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... The spin-labeled proteins were further purified by HiLoad 26/600 Superdex 200 pg column (GE life sciences) in a buffer of 150mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified and excess biotin removed from proteins biotinylated in vitro by size exclusion chromatography (Superdex 75 or 200 column [GE Healthcare]) in HBS-EP ...
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bioRxiv - Cell Biology 2020Quote: ... 35S-labeled proteins were separated by SDS-PAGE and detected with an Autoimaging analyzer (Typhoon FLA-9500; GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The labeled proteins were separated from the free dyes on a homemade Sephadex G50 resin (GE Healthcare, Piscataway, NJ) column in TBS buffer.
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with standard erythrocyte lysis buffer on ice and separated from myelin and debris by Percoll gradient (GE Healthcare) centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... Fluorescence profiles and peak volumes of labeled proteins were obtained using ImageQuant TL v2005 (GE Healthcare Life Sciences, Piscataway, NJ). For calculation of AA74-1 IC50 values (Fig ...
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bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... the labeled protein (M1-Alexa488) was separated from the free dye using size-exclusion chromatography (PD-10, GE Healthcare, Munich, Germany). The labeled protein was eluted with diluted PBS (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... The gels were exposed to imaging plates and the images of separated tracer-labeled proteins were obtained by reading the plate using a Typhoon 9410 (GE Healthcare) image analyzer ...
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Physiology 2020Quote: ... The labeled protein was subsequently purified using size exclusion chromatography on a Sephadex™ G-25 Medium column (GE Healthcare, UK). To remove excess free dyes from the labeled proteins and also avoid hydrolysis of extra fluorophore ...
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...