Labshake search
Citations for GE Life Sciences :
51 - 100 of 4182 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... pH 7.4 400 mM Imidazole) The labeled protein was further purified by size-exclusion chromatography (ÄKTA pure, Superdex-75 Increase 10/300 GL, GE Healthcare) to eliminate remaining fluorophores and remove other contaminants and soluble aggregates ...
-
bioRxiv - Cell Biology 2019Quote: ... 6His-Cdc11-SNAP was biotinylated with BG-Biotin and subsequently immobilized on a CAP chip (Biotin Capture Kit; GE Healthcare, Freiburg, Germany). Preparation of the chip ...
-
bioRxiv - Biochemistry 2022Quote: ... attached to an ÄKTA basic HPLC system equipped with a UV-900 detector (GE Healthcare). The column was equilibrated with 50 mM Tris HCl ...
-
bioRxiv - Microbiology 2020Quote: ... The labeled protein was separated from the excess of free 125I in a PD10 desalting column (GE Healthcare Life Sciences, United Kingdom) and the purity of the 125I-labeled Vip3Aa was checked by autoradiography ...
-
bioRxiv - Biophysics 2020Quote: ... Unconjugated TFP ester dyes were separated from labeled proteins using HiTrap Desalting columns with Sephadex G-25 resin (GE Healthcare Life Sciences). Alexa Fluor™ 647 C2 Maleimide (A20347 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Free proteins were labeled following the manufacturer’s instructions and purified using Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences).
-
bioRxiv - Cell Biology 2022Quote: ... 50 μg of protein per sample were solved in urea lysis buffer and labeled with 250 pmol CyDye DIGE Fluor dyes (GE Healthcare, Germany). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
-
bioRxiv - Cell Biology 2021Quote: ... Cy3-labeled α-goat (GE Healthcare), red TEXAS α-rabbit (Jackson Laboratories ...
-
bioRxiv - Biophysics 2019Quote: ... The mAb TS2/4-biotin was purified from an excess of NHS-LC-LC-Biotin via gel filtration (Superdex-200 10/300; GE Healthcare Life Sciences), concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
-
bioRxiv - Biophysics 2023Quote: Both mFE- and tFE-labeled proteins were further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
-
bioRxiv - Neuroscience 2021Quote: ... labeled with Cy3 dUTPs (GE healthcare: PA53022), by means of nick translation (Abbott ...
-
bioRxiv - Microbiology 2024Quote: ... Secondary peroxidase labeled α-mouse (GE Healthcare), α-rabbit (GE Healthcare ...
-
bioRxiv - Biophysics 2020Quote: The oligomeric state of IM30* and IM30 fragments was analyzed using an ÄKTA basic system (GE Healthcare) with a Superose12 10/300 GL column (GE Healthcare ...
-
bioRxiv - Neuroscience 2020Quote: ... was labeled with 99mTc (GE Healthcare, Seattle, WA) using the stannous tartrate method and the 99mTc – labeled albumin (T-Alb ...
-
bioRxiv - Biophysics 2021Quote: ... The capture probes from Qiagen were labeled with Cy3 monoreactive dye (GE Healthcare) and purified by ethanol precipitation and washing with 80% ethanol until the supernatant was colorless ...
-
bioRxiv - Neuroscience 2022Quote: ... was labeled with 99mTc (GE Healthcare, Piscataway, NJ). A mixture of 240 mg/ml stannous tartrate and 1 mg/ml albumin was adjusted to pH 3.0 with HCl and one millicurie of 99mTc-NaOH4 was added to this mixture and incubated for 20 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... and probed with 5’ end-labeled oligonucleotides or PCR fragments labeled by random priming (Megaprime DNA Labeling System, GE Healthcare Life Sciences). Hybridizations were performed in UltraHyb or UltraHyb-Oligo (Ambion ...
-
bioRxiv - Immunology 2022Quote: ... labeled according to the manufacturer’s recommended protocols (GE Healthcare). Arrays were immersed in a hybridization solution containing a mixture of Cy3-rabbit antihuman IgG solution diluted 1:1000 in wash buffer for 2 h at 50 rpm at 20°C ...
-
bioRxiv - Immunology 2020Quote: ... a diluted secondary antibody labeled with HRP (GE healthcare) was added and incubated for 3 hours at RT ...
-
bioRxiv - Biochemistry 2023Quote: ... Labeled INIP was purified using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with INIP storage buffer (25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were labeled with Cy3 NHS ester (GE Healthcare) at an amine-modified internal thymine and HPLC purified on a 218TP C18 reverse-phase column (Grace/Vydac ...
-
bioRxiv - Cell Biology 2023Quote: ... Horseradish peroxidase-labeled anti-M13 monoclonal antibody (GE Healthcare) and Thermo Scientific 1-Step Ultra TMB solution were used for detection of bound phages ...
-
bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
-
bioRxiv - Microbiology 2019Quote: ... Biotin excess was removed by SEC on a Superdex 200 column (GE Healthcare). In the SEC profile ...
-
bioRxiv - Biophysics 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare).
-
bioRxiv - Plant Biology 2022Quote: ... followed by the removal of biotin by desalting on PD10 columns (GE Healthcare). The desalted lysates were then applied to streptavidin agarose resin (100 µl ...
-
bioRxiv - Immunology 2022Quote: ... excess biotin and ligase were removed with a Superdex 200 column (GE Healthcare). The control MHC-I (HLA-A2 ...
-
bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies were Cy3-labeled α-mouse (GE Healthcare), fluorescein-conjugated α-rat (Millipore) ...
-
bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-labeled anti-mouse IgG antibody (GE healthcare) was used as a secondary antibody ...
-
bioRxiv - Molecular Biology 2023Quote: ... Labeled octamer was purified over a Superdex 200 (GE Healthcare) gel filtration column to remove excess dimer ...
-
bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG horseradish peroxidase labeled secondary antibody (GE Healthcare). Proteins were detected by ECL chemiluminescent detection system (Millipore ...
-
A nascent riboswitch helix orchestrates robust transcriptional regulation through signal integrationbioRxiv - Molecular Biology 2024Quote: NusA was labeled with Cy5-maleimide dye (PA25031, GE Healthcare) using an 8:1 molar ratio of dye to protein in a total volume of 200 µL (∼20 µM protein ...
-
The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... Labeled RNA was purified on a G50-column (GE Healthcare) and extracted from a PAA gel as described above.
-
bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was loaded on a Superdex 200 16/600 (GE-healthcare) size exclusion column connected to an AKTA Basic FPLC (GE Healthcare) in SEC-buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... polymerized from a 1:20 ratio of Cy5-labeled (GE Healthcare) and unlabeled tubulin ...
-
bioRxiv - Microbiology 2020Quote: ... were labeled using the Cy5 monoreactive Dye pack (PA25001, GE Healthcare) and filtered using Illustra microspin G25 columns (27532501 ...
-
bioRxiv - Biochemistry 2021Quote: ... were directly labeled with Cy3 following the manufacturer’s guidelines (GE Healthcare). After 45 min incubation in the dark ...
-
bioRxiv - Biochemistry 2020Quote: The N-terminus of Rad6 was labeled with Cy5 (GE Healthcare). Briefly ...
-
bioRxiv - Microbiology 2022Quote: LecB was labeled with Cy3 mono-reactive NHS ester (GE Healthcare) or with biotin using NHS-PEG12-biotin (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... Labeled oligonucleotides were purified using microspin G-25 columns (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Labeled RNA was purified on a G-50 column (GE Healthcare), following separation in and extraction from a polyacrylamide gel as described above.
-
bioRxiv - Biochemistry 2020Quote: ... Unincorporated biotin and aggregated viruses were removed by NAP-5 gel filtration columns (GE Healthcare) and 0.2 μm pore size filters ...
-
bioRxiv - Microbiology 2021Quote: ... Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare). In the SEC profile ...
-
bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... All Cy5-labeled DNA was detected using Fujifilm FLA-9000 (GE Healthcare). Competition assays using specific and nonspecific oligos were performed as previously described [11] using 0.2-4 μM of competitor ...
-
bioRxiv - Microbiology 2021Quote: ... labeled probes were purified using Illustra MicroSpin G-50 columns (GE Healthcare) and 40 pmol of these probes was added to the blocked membranes ...
-
Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... Labeled oligonucleotides were further purified via Microspin G-25 columns (GE Healthcare) to remove unincorporated 32P-γ-ATP ...
-
bioRxiv - Molecular Biology 2020Quote: ... The FAM-labeled products were imaged via Amersham Typhoon scanner (GE Healthcare) and the radioactive labeled products were visualized by phosphorimaging.
-
bioRxiv - Cell Biology 2020Quote: ... Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare), anti-mouse IgG (GE Healthcare ...