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Citations for GE Life Sciences :
1651 - 1700 of 2764 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Molecular Biology 2023Quote: ... Then the samples were subjected to electrophoresis through 5% native polyacrylamide gel in 0.5x TBE buffer and imaged with 9410 Typhoon variable mode imager (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The purified protein was then dialyzed against 10 mM Tris HCl pH 8.0 and loaded on MonoQ 5/15 column (GE Healthcare) to be eluted using a step gradient of 240 mM and 400 mM NaCl in the same buffer.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged at 40,000 rcf for 1 h and the supernatant was filtered through a 0.45 μM syringe filter (Chromafil GF/PET 45, MachereyNagel) and passed through a HiTrap SP HP (5 mL, GE Healthcare) column connected to an NGC FPLC system (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... the Ni-NTA eluate was applied to size exclusion chromatography on a Superose 6 Increase 5/150 column (GE Healthcare) in basic buffer supplemented with 0.003 % LMNG and 0.0003 % CHS.
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bioRxiv - Biochemistry 2023Quote: ... The lysate was passed through a 0.22 µm filter and loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 25 ml of lysis buffer containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: Elute from HisTrap consisting of the respective protein fractions were centrifuged at 39,000 g for 15 minutes and the supernatant was loaded on a 5 ml StrepTrap HP column (GE Healthcare). The binding buffer used was 150 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was diluted 5-fold to contain 100 mM NaCl and purified over SP-Sepharose (HiPrep SPFF, GE Healthcare) using a linear gradient of buffer containing 2 M NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 and equilibrated with wash buffer (50 mM K-HEPES pH7.5 ...
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bioRxiv - Biophysics 2023Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono S 5/50 GL, GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M was applied over 20 min and the protein was eluted in 5 ml fractions ...
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bioRxiv - Biophysics 2023Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using CL-Xposure films (Cat.no ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted fractions containing the desired protein were pooled and loaded onto a 5 ml cationic exchanger (S-column; GE Healthcare). A salt gradient of 0-1 M KCl was applied over 20 min (at 5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was loaded in an ÄKTA Pure purification system (Cytiva) onto a 5 ml HiTrap Chelating HP (GE Healthcare) charged with 0.1 mM NiCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) in an S75 5/150 GL increase column (GE Healthcare). Protein collected from SEC was normalized to 1 μM where possible.
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bioRxiv - Biophysics 2024Quote: ... HRP-secondary antibodies were incubated for 1-2 hours at room temperature in 5% milk and ECL were performed using the Amersham ECL Western Blotting Detection Reagents (Cat.no. RPN2106 from GE Healthcare). Detection was done using the Chemidoc XRS imaging system (Bio-Rad)..
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bioRxiv - Microbiology 2023Quote: ... the KhpA and KhpB-ΔN were secondly purified by cation-exchange chromatography (HiTrap SP 5 ml, GE Healthcare Life Science) followed by SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... the protein was filtered and loaded onto two connected 5 ml HiTrap Q HP columns (GE Healthcare, 17- 1154-01) equilibrated with 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Biophysics 2024Quote: ... Double-labeled (488:594) protein was then further purified via ion-exchange chromatography (Mono Q 5/50 GL, GE Healthcare).
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... Bands were visualized by horseradish-peroxidase-conjugated sheep anti-mouse IgG antibodies (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare), washed and purified according to standard ChIP procedures ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary antibodies were incubated with ECL Prime enhanced chemiluminescent substrate (GE Healthcare) and visualized by exposure to autoradiography film ...
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bioRxiv - Cancer Biology 2019Quote: ... The clicked antibodies were purified by SEC (Superdex 200, 10×300 GL, GE Healthcare) at a flow rate of 0.5ml/min in PBS using a GE AKTAPurifier ...
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bioRxiv - Biochemistry 2019Quote: ... and coverslips were incubated with 1:100 primary anti-bromodeoxyuridine antibody (RPN202; GE Healthcare) in the dark for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were detected by binding goat anti-rabbit IgG-HRP (NA934, GE Healthcare), goat anti-mouse IgG-HRP (NA931 ...
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bioRxiv - Biochemistry 2020Quote: ... and IgG anti-rabbit horseradish peroxidase–conjugated secondary antibody (1:10,000; GE Healthcare UK). For whole cerebellum and cerebellar slices ...
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bioRxiv - Cancer Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931, GE Healthcare Life Sciences, 1:10,000) or HRP-conjugated anti-rabbit antibody (NA934V ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by an anti-rabbit-IgG HRP linked whole antibody (GE Healthcare, Chicago, IL). The MERS-CoV viral lysate was immuno-detected with a MERS-coronavirus spike protein S2 polyclonal antibody (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... probed with primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated IgG (GE Healthcare), and finally visualized by enhanced chemiluminescence (Western Lightning ECL-Pro ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibody binding was followed by anti-mouse (GE Healthcare, catalog # NXA931V, lot # 17041890) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... we used a peroxidase-labeled anti-rabbit antibody (NIF824, GE Healthcare; 1:5000 dilution). Immunodetection was performed by incubating the membranes in the Western BLoT Quant HRP Substrate (Takara ...
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bioRxiv - Microbiology 2020Quote: ... sheep anti-mouse IgG antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, Code#: NA931). For detection of ClpP ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10000 dilution of HRP-conjugated donkey anti-rabbit antibody (GE Healthcare, NA934), and ECL detection reagent ...
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bioRxiv - Microbiology 2019Quote: ... IgG antibodies were purified from the supernatant using a Protein G column (GE Healthcare). The subclass of each IgG antibody was determined using a human IgG subclass ELISA kit (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Detection was achieved with a HRP-conjugated rabbit or mouse secondary antibody (GE Healthcare) followed by enhanced chemiluminescence with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000, #NA931, GE Healthcare). The soluble fraction was found to contain more AβM(E22G)-mCherry than the insoluble fraction (Supplementary Figure 5. ...
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bioRxiv - Cancer Biology 2021Quote: ... the anti-rabbit IgG-HRP and anti-mouse IgG-HRP antibodies from GE Healthcare, and the anti-HA antibody 12CA5 from Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... followed by the corresponding Horseradish Peroxidase (HRP) conjugated secondary antibodies (1:5000, GE Healthcare) and visualized using the ECL Plus Western Blotting detection system (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... anti-rhodopsin antibody (1D4) to CNBr-activated Sepharose 4B beads (GE Healthcare Bio-Sciences) according to manufacturer’s instructions (Molday and Molday ...