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Citations for GE Life Sciences :
1501 - 1550 of 2764 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Serum IgG antibodies were purified by protein G column chromatography (GE Healthcare, USA). The purified IgG antibodies were detected by SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were donkey anti-rabbit horseradish peroxidase (GE Healthcare NA934, 1:2,000) and goat anti-rat horseradish peroxidase (GE Healthcare NA935 ...
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bioRxiv - Immunology 2022Quote: ... HRP-conjugated mouse (NA931) and rabbit (NA934) secondary antibodies were from GE Healthcare; FemtoLUCENT™ PLUS HRP Kit (cat no ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal antibodies were immobilized on Series S Protein G sensor chip (GE Healthcare) at a density of 2,000 response units (RU) ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibodies were mouse IgG HRP-linked (NA931, GE Healthcare; 1:5000) or rabbit IgG HRP-linked (NA934 ...
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bioRxiv - Plant Biology 2023Quote: ... the antibodies were further affinity purified using Protein G mag sepharose (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... followed by HRP-linked secondary antibodies (1:5,000) and enhanced chemiluminescence (GE healthcare). α-His antibody (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were purified from hybridoma supernatant using HiTrap Protein G columns (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... Bound antibodies reacted with Amersham ECL Prime (GE Healthcare Life Science, PA, USA) and were detected using ImageQuant LAS 4000 mini (GE Healthcare Life Science) ...
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bioRxiv - Bioengineering 2024Quote: ... or G12p4 antibodies were immobilized on a CM5 sensor chip (GE Healthcare Technologies) with an amine-coupling kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Protein bands reacting with antibodies were visualized using chemiluminescent detection reagent (GE Healthcare) on an iBright FL1500 (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibody ECL anti-mouse IgG (GE Healthcare Life Sciences, Buckinghamshire, UK), diluted 1:5000 in TBS buffer containing 5% milk powder was applied to the membrane for 90 minutes at room-temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The secondary antibody ECL anti-mouse IgG (GE Healthcare Life Sciences, Buckinghamshire, UK), diluted 1:5000 in TBST containing 5% milk powder was applied to the membrane for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... a fluorescent Cy5-conjugated goat anti rabbit secondary antibody (GE Healthcare; 1:5,000) was used and the fluorescent signal acquired with a Typhoon fluorescent scanner (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: HRP conjugated anti-mouse and anti-rabbit (GE Healthcare, Chicago, IL).
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bioRxiv - Molecular Biology 2023Quote: ... along with corresponding species IgG secondary antibodies (1:5000, GE Healthcare, Fisher Scientific) were utilised ...
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bioRxiv - Cancer Biology 2023Quote: ... The antigen-antibody complexes were visualized using an enhanced chemiluminescence system (GE Healthcare) and captured by Azure 500 system (Azure Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... Signals were developed with horseradish peroxidase-conjugated secondary antibodies (GE Healthcare Bio-sciences) or peroxidase-conjugated primary antibodies using Western Lightning Plus-ECL (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... The antibodies were cross-linked to Protein G Mag Sepharose Xtra (GE Healthcare) according to the manufacturer’s instructions before immunoprecipitations ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody binding was detected by chemiluminescence using the ECL detection system (GE Healthcare) or ECL Plus (for NICD ...
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bioRxiv - Immunology 2024Quote: ... wells were washed and incubated with horseradish peroxidase (HRP)-conjugated antibodies (GE Healthcare) for 3 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with HRP-conjugated anti-mouse secondary antibodies (Ge Healthcare, USA) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibodies used were horseradish peroxidase-conjugated anti-mouse (NA931; GE Healthcare) and anti-goat (ab6741 ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was then supplemented with 5 mM imidazole prior to loading onto a 1-ml HisTrap column (GE Healthcare) attached to an AKTA system at 1 ml/min ...
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bioRxiv - Microbiology 2021Quote: ... The resultant eluate was filtered through a 0.45 μm filtration membrane and then loaded onto a 5 ml SOURCE 15S column (GE Healthcare) equilibrated with buffer I ...
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bioRxiv - Biophysics 2020Quote: IgG was isolated from porcine sera by passing the sera over a MabSelect 5 mL HiTrap Column (GE Life Sciences), washing with two column volumes of tris-buffered saline and elution with 0.1 M glycine ...
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bioRxiv - Biophysics 2019Quote: The proteins and protein complexes were analyzed by analytical gel filtration using a Superdex 200 5/150 column (GE Healthcare) and the 1260 Infinity Bio-inert high-performance liquid chromatography system (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
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bioRxiv - Microbiology 2020Quote: ... The cleared membrane fraction was loaded with a flow of 0.8 mL/min on a pre-equilibrated 5 mL StrepTrap column (GE healthcare) mounted on as AktaPurifier system (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... images were obtained every 5 min for 4 h using a 20X objective (0.75 NA, PlanApo) on a DeltaVision microscope (GE Healthcare) equipped with a CoolSNAP HQCCD camera (Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Molecular Biology 2021Quote: ... radioactive signal was impressed on a FUJIFILM Storage Phosphor screen for 5 hours and acquired with Typhon Trio (GE healthcare).
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bioRxiv - Biophysics 2022Quote: ... The RNA samples were first purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare, Chicago, IL) at pH 7.0 then annealed in buffers containing 40 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and the supernatant was loaded onto a 5-ml bed volume of HiTrap Heparin column (GE Healthcare, Pittsburgh, PA, USA) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... reacted with 5 to 20-fold excess of Alexa-568 or Alexa-488 NHS ester (catalog # A20003, A20000, GE Healthcare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the sample was concentrated to 5 ml and loaded over the HiLoad 26/600 Superdex 200 prep grade (GE Healthcare). In order to clear the impurities which had been eluted with the complex ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered through a 0.45 µm syringe filter and applied onto a 5 ml MBPtrap column (GE Healthcare). The column was washed with 10 CV of lysis buffer and the protein was eluted with 5 CV of lysis buffer supplemented with 15 mM maltose ...
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bioRxiv - Immunology 2021Quote: ... the supernatant of stable HEK293S cells expressing αMβ2 headpiece was recovered and purified by immobilized ion-affinity chromatography using a 5 mL HisTrap Excel column (GE Healthcare). The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... The bacterial lysate was clarified using centrifugation at 20,000 x g for 40 min and loaded onto a 5 ml His-Trap column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Complexes were then run using 5% native PAGE in 0.5× TBE and visualized using the Typhoon 9400 variable mode laser scanner (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... Metaphase cells were imaged for 5 min every 7.5 s using an Olympus DeltaVision wide-field microscope (GE Healthcare, Switzerland) equipped with a eGFP/RFP filter set (Chroma) ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing the periplasmic extract was sonicated before being loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with stepwise increases of imidazole in buffer E (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... then supernatants were filtered (0.45 μm syringe filter, Iwaki) prior to purification on 5 ml HiTrap Chelating HP columns (GE Healthcare). Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T were obtained from ATCC and cultured at 37°C and 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2020Quote: siRNA-mediated knockdown in 786-O cells was carried out using 5-10 nM Dharmacon ON-TARGETplus siRNAs (GE Healthcare), diluted in siRNA buffer and mixed with DharmaFECT transfection reagent (1:50 in optiMEM reduced serum media) ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 μg proteins were resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). To visualize MLKL oligomers ...
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bioRxiv - Genetics 2019Quote: ... 20 μg of PCR products was immobilized to 5 μl Streptavidin Sepharose High Performance Bead (GE Healthcare, Piscataway, NJ, USA), and subsequently annealed to 1 μl sequencing primer (5 μM ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was boiled at 98 °C for 5 min, applied to SDS page gel (SuperSep, WAKO) and transferred onto a 0.2 µM nitrocellulose blotting membrane (GE Healthcare). After incubation with blocking buffer (5% milk in tris-buffered saline with 0.1% Tween20 ...
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bioRxiv - Cancer Biology 2019Quote: ... the lysate spun down at 12,000 x g for 30 min and then loaded onto a 5 ml His-Trap Crude FF column (GE Healthcare) using a ÄKTA Purifier protein purification system (GE Healthcare) ...