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Citations for GE Life Sciences :
1801 - 1850 of 2694 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... WCE was then run on 5–20% gradient SDS–polyacrylamide gel electrophoresis (PAGE; ATTO) and then transferred to Hybond ECL membranes (GE Healthcare) followed by incubation with indicated antibodies ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions were pooled and purified by anion-exchange on a 5 ml Hitrap Q (Cytiva, formerly GE Healthcare Life science) in a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Molecular Biology 2023Quote: ... Further protein purification was achieved by performing size-exclusion separation of 5 mL of the eluate from the affinity chromatography step on a Hiload 16/60 Superdex S200 column (GE Healthcare), with the use of 1.2 column volumes of gel-filtration (GF ...
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bioRxiv - Microbiology 2023Quote: ... The TEV digested proteins were separated from the cleaved His-tag product on an AKTA Pure 25L FPLC using a 5 mL HisTrap-FF with the captured flowthrough further purified using a HiLoad Superdex 75 PG gel filtration column (GE Healthcare). A monodisperse peak was obtained and assessed for purity using SDS-PAGE gel analysis ...
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bioRxiv - Microbiology 2023Quote: ... PmScsC-EcDsbA was initially purified by immobilized metal affinity chromatography (IMAC) using a 5 mL HisTrap Fast Flow column (GE Healthcare) followed by cleavage of the N-terminal His6-tag with TEV protease and reverse IMAC ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 50 mM NaCl and loaded on an equilibrated 5 ml HiTrap Heparin HP Sepharose column (GE Healthcare). The protein was eluted using a linear 0–1 M NaCl gradient in buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... injected (5 mL total) into HiLoad 16/600 Superdex 75pg (Cytiva) connected to an AKTA FPLC system (GE Healthcare Life Science). Gel filtration was performed using the same buffer to purify Nurr1-RXRα LBD heterodimer for analysis ...
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bioRxiv - Biochemistry 2023Quote: ... DAP5 was first purified from bacterial cell lysate using a 5 mL His-Trap HP (Ni-NTA) column from GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... rPF4 was purified from soluble fractions of cell lysates by affinity chromatography using a 5-ml HiTrap heparin-agarose column (GE Healthcare). Endotoxin-free rPF4 was prepared as previously described 3 ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 4 °C overnight as described above and scanned with GE Amersham Typhoon-5 fluorescence gel and blot scanner (GE Healthcare) with red laser.
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bioRxiv - Biophysics 2023Quote: ... Pooled fractions from ion-exchange chromatography were filtered through a 5 µm syringe filter and loaded onto a 22 mL Source 15ISO hydrophobic interaction column (GE Healthcare) equilibrated in buffer C (50 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... The soluble fraction was filtered through a 0.45 μm sterile syringe filter and loaded onto a pre-equilibrated 5 mL HiTrap IMAC HP column (GE Healthcare Biosciences). The column was washed with 4 column volumes of lysis buffer followed by 4 column volumes of wash buffer 1 [50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... Transcription template and HDV ribozyme with T7 promoter sequences were cloned into pUC57 vector and the extracted plasmids were further purified with Source Q 5/50 GL column (GE Healthcare). The DNA fractions were precipitated with 0.3 M NaAc and 70% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μM of OCP and 20 μM of FRP were pre-incubated in SEC buffer for 15 min at room temperature and then loaded onto a Superdex 200 Increase 5/150 column (GE Healthcare) pre-equilibrated with the same buffer at 0.45 ml/min ...
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bioRxiv - Biophysics 2023Quote: ... The His6-SUMO-14-3-3ζ fusion protein was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose 6 Fast Flow resin (5 mL; GE Healthcare) with standard IMAC procedures of sample application ...
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bioRxiv - Molecular Biology 2023Quote: ... further clarified using 0.2 µm filter and loaded on 5 ml Ni-NTA Sepharose high-affinity pre-packed column (His TrapTM, GE healthcare, Sweden), which was pre-equilibrated with binding buffer (20 mM Tris-Cl ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Microbiology 2023Quote: ... centrifuged 30 min at 2150g and concentrated/diafiltrated against loading buffer (D-PBS, 500 mM NaCl, 5 mM Imidazole) using the AKTA crossflow system (GE healthcare) supplemented with a 10KDa ...
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bioRxiv - Cell Biology 2023Quote: ... Rab35BD and Rac1BD-Rab35BD) (40 µL; 10-25 mg/ml) were loaded onto a Superdex-200 increase 5/150 column (GE Healthcare) or BioSec5 300 (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... After elution with 500 mM imidazole the VHH-containing fractions were buffer-exchanged with PBS using a Vivaspin column (5 kDa cutoff, GE Healthcare). The purified VHHs were analyzed by SDS-PAGE and Coomassie staining and by intact mass spectrometry ...
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bioRxiv - Biophysics 2023Quote: ... lysed with a high-pressure homogenizer and Fab was purified with a 5-ml HiTrap Protein G HP column (GE Healthcare). Pooled fractions were dialyzed against sodium carbonate buffer (pH 5.0 ...
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bioRxiv - Biophysics 2023Quote: ... The successfully cleaved samples were subsequently buffer exchanged using a Superdex S200 5/150 GL or 10/300 increase column (GE Healthcare) connected to an Agilent HPLC 1260 Affinity equipped with a fraction collector ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Cell Biology 2023Quote: ... and pure fractions were pooled and concentrated before being concentrated and snap-frozen in liquid nitrogen GST-tagged RAB7A was similarly isolated via passing cleared lysate over a gravity column packed with 5 mL of Glutathione Sepharose 4B (GE Healthcare), followed by elution using 10 mM reduced glutathione dissolved in 50 mM Tris-HCl pH 8.0. ...
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bioRxiv - Microbiology 2023Quote: ... The TLC plate was left in the TLC chamber until the solvent front was approximately 5 cm from the top of the TLC plate and finally phosphorimaged using a Typhoon FLA 7000 imager (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The protein-containing supernatant was collected and filtered through a 0.45 μm size cut-off filter and applied onto a 5 mL His Trap™ crude FF column (GE Healthcare), running in equilibration buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and the soluble His6-M protein was purified on a Nickel sepharose column (HiTrap™ 5 ml IMAC HP; GE Healthcare). The bound protein was washed extensively with loading buffer plus 25 mM imidazole and eluted with a 25 to 250 mM imidazole gradient ...
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bioRxiv - Biochemistry 2023Quote: The filtered cell-free extract was subjected to immobilized metal affinity chromatography using an Äkta pure chromatography system equipped with a 5-mL HisTrap HP column (GE HealthCare) equilibrated with lysis buffer (see above) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 M NaCl and 30 mM imidazole and captured with Glutathione-Sepharose 4B beads as indicated by the manufacturer (GE Healthcare). The elution fractions were pooled and the buffer was exchanged to phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Capturing of ORF1p-His6 protein was performed using Immobilized-Metal Affinity Chromatography (IMAC) by loading of 7M Urea Extract on 5 mL HisTrap FF FPLC column (GE Healthcare) at 0.5 mL/minute flow rate followed by washing with 10 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... was performed at 20 °C using S75 (IDR constructs) and S200 Increase 5/150 (NTD and ARD constructs) analytical SEC columns (GE Healthcare) equilibrated in the appropriate buffers (see Tables S1 and S2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then washed in PBS-T and incubated with 1 mL of Enhanced ChemiLuminescence (ECL) (Amersham) reagent for 5 minutes to allow protein visualization on an x-ray Amersham Hyperfilm ECL (GE Healthcare). Fiji-Image J Software was used for quantification of the difference in protein expression comparing with GAPDH or HSP70 expression.
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bioRxiv - Biophysics 2023Quote: ... diluted with the same buffer devoid of NaCl to reach a final salt concentration of 40 mM and loaded on a HiSure Q 5 mL column (GE Healthcare). After washing with a 25 mM Na-HEPES ...
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bioRxiv - Microbiology 2023Quote: ... Blots were developed with standard BCIP (5 % (w/v) in DMF) + 250 μL NBT (5 % (w/v) in 70 % DMF) or using the ECL western blotting reagent (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Lysate was cleared by centrifugation at 20000g for 45 min and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–300 mM in 30 column volumes ...
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bioRxiv - Immunology 2023Quote: ... 6 and 14 we euthanized 5 individuals per group and performed an intra-cardiac terminal blood collection for PBMCs isolation with Ficoll-Paque Plus (GE healthcare). We performed a complete necropsy of all animals and collected samples of the lungs ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h prior to exposure to a phosphor-screen for ∼5 h to overnight and imaged using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The fractions collected at the elution peak were pooled together and loaded into a MonoS 5/50 GL column (GE Healthcare) previously equilibrated with IEX Equilibration buffer (50 mM HEPES pH7.8 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Neuroscience 2024Quote: ... the membranes are subjected to five more washes in TBS-T before being incubated in chemifluorescence substrate for a maximum of 5 minutes and scanned on either a Storm 860 Scanner (Amersham Biosciences) or Typhoon FLA 9000 Scanner (GE Healthcare) or being visualized in a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation with 20,000 x g followed by filtration with a 5 μM filter and loaded on a HisTrap HP column pre-equilibrated with lysis buffer (GE Healthcare). The column was washed first with lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation with 20,000 x g at followed by filtration with a 5 μM filter before loading on a HisTrap HP column (GE Healthcare). The column was washed first with lysis buffer ...