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Citations for GE Life Sciences :
1601 - 1650 of 2318 citations for AMPK alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was incubated for 1 hour with horseradish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Piscataway, NJ). The target protein on the membrane was detected by Pierce™ ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM DTT) and then loaded on a HiTrap Heparin column (CV, column volume 5 ml; GE Healthcare), equilibrated with buffer A ...
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bioRxiv - Neuroscience 2020Quote: ... monkey (XP_005545315.1; 121-760aa) and human (NP_001121620.1; 120-760aa) TfR1 in 0.1% BSA in HBS-EP+ buffer (GE Healthcare) was captured at flow rate 10 μl/min ...
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bioRxiv - Cell Biology 2019Quote: ... filtered through a 0.22 µm filter and loaded onto a HiTrap IMAC FF 1 ml column (GE Healthcare) charged with Ni2+ and attached to an AKTA system ...
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bioRxiv - Microbiology 2019Quote: ... The resulting conidial suspension was filtered through Whatman 1 filter paper (Whatman plc, GE Healthcare Life Sciences, UK) to remove hyphae and debris ...
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bioRxiv - Synthetic Biology 2020Quote: The DNA fragment encoding the PreS1 domain (residues 1-56) was cloned into the pGEX-2T (GE Healthcare) plasmid ...
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bioRxiv - Zoology 2020Quote: ... for 1 h at room temperature and developed in ECL (Amersham ECL Select; GE Healthcare, Chicago, IL, USA). Bands were visualized with the MultiImage III FluorChem Q (Alpha Innotech ...
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bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were then incubated with anti-Mouse HRP-conjugated secondary antibody (1:5000 of NA931, GE Healthcare) for 1 hour at room temperature followed by three washes with PBST ...
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bioRxiv - Microbiology 2020Quote: ... MS3_01370 was purified by IMAC by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... 30 μl 1× TE was added and the probe spun through a G-50 spin column (GE Healthcare) at 400 × g for 2 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: The clean lysate was next loaded onto a HisTrap HP column (HisTrap FF crude 1 ml, GE Healthcare) at 4°C and flow rate of 0.5 ml min−1 ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; GE Healthcare, Freiburg, Germany). An electrochemiluminescence assay (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Method 1 (H-P): Genomiphi™ V2 DNA Amplification Kit (Cytiva illustra™, formerly GE Healthcare Life Sciences) was used for whole genome amplification according to manufacture instruction ...
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bioRxiv - Immunology 2020Quote: Buffy coats were diluted [1:3] with PBS and added to Leukosep tubes containing Ficoll-Paque (GE Healthcare). Tubes were centrifuged at 450g for 40 minutes and the resulting buffy layer extracted ...
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bioRxiv - Microbiology 2020Quote: ... Samples (100 uL) at 1 mg/mL were injected and run over a Superdex 200 GL (GE Healthcare) column at a 0.5 ml/min flow rate ...
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bioRxiv - Microbiology 2020Quote: ... The NEC220 and NEC-CBM constructs were passed over 2 x 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange as previously described ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies used for western blotting are as follows: α-mouse-HRP (1:1000) (GE Healthcare, Piscataway, NJ) for α-O-GlcNAc primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated with complementary HRP conjugated secondary antibody (sheep anti-mouse IgG-HRP, 1:10000, GE Healthcare, NA931 ...
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bioRxiv - Biochemistry 2022Quote: ... to < 1 mL and injected onto a Superdex™ 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 1-2 minutes in darkness then exposed and imaged using an Amersham™ Imager 600 (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was passed through a filter paper (Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), 1 mL of the flow through was transferred into a 1.5 mL centrifuge tube ...
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bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3h at room temperature in streptavidin horseradish peroxidase (HRP) conjugate (GE Healthcare, 1:200) diluted in 0.3% Triton X-100 in 0.01 M PBS ...
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bioRxiv - Genetics 2024Quote: ... the membranes were incubated with a horseradish peroxidase-conjugated against the appropriate species antibody (1:10,000; GE Healthcare) diluted in the blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then probed with horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, GE Healthcare, NA934, RRID: AB_772206) for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: All images except for those in Figure 1 were acquired using a DeltaVision Personal DV system (Applied Precision) equipped with a 100X N.A ...
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bioRxiv - Microbiology 2023Quote: ... were used in combination with secondary anti-mouse antibodies conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). For visualization ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM MgCl2 + 10% glycerol) was injected manually into a Superdex 200 Increase 10/300 GL (GE Healthcare) matrix column at a constant flowrate of 0.4ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... to < 1 mL and injected onto a Superdex™ 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed solution was then loaded on a 1 ml HisTrap SP FF column (GE Healthcare, Pittsburgh, PA) and eluted using a linear gradient of NaCl (20-500 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was then digested with 1 mL of enzyme-free dissociation agent HyClone™ (GE Healthcare Life Sciences) for 5 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... a condition using non-targeting siRNA (ON-TARGETplus Non-targeting Control siRNA #1, from Dharmacon, GE Healthcare, USA) was used at the same concentration as the siRNA targeting the genes of interest ...
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bioRxiv - Biophysics 2024Quote: ... (1994) but used FPLC steps of heparin-affinity using a HiTrap Heparin-HP 1 ml column (GE Healthcare), followed by anion exchange chromatography using a MonoQ 5/50GL (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... RRM2 (>5 mg mL-1) was injected onto a 24 mL Superdex 75 size exclusion column (GE Healthcare) at a flow rate of 1 mL min-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... DprA was purified from the clarified lysate by two-step chromatography using HisTrap HP 1 mL (GE Healthcare) and HiTrap SP XL 1 mL columns (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM TCEP] by size-exclusion chromatography using a HiLoad 16/60 Superdex 200 gel-filtration column (GE Healthcare). PelXC232S eluted as a single Gaussian shaped peak ...
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bioRxiv - Cell Biology 2020Quote: ... In a 48 well plate, 25-32.5μL siRNA (for sequences, see table 1) was mixed with 25uL 1x siRNA buffer (GE Healthcare) containing 0.5uL Dharmafect 1 transfection reagent ...
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bioRxiv - Developmental Biology 2021Quote: ... For monoclonal antibody production, the DNA encoding mouse KCTD19 (residue 654-793 aa, NP_808459.1) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and fractions contacting samples were loaded onto size-exclusion column (Superdex 75 16/60, GE Healthcare) pre-equilibrated with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... Three washes were performed with TBST followed by 1 h incubation with secondary antibody (ECL, HRP linked, GE Healthcare) and another series of washes ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was loaded at 1 ml/min on a His-Trap FF-crude 5 mL column (GE Healthcare) equilibrated with PBS buffer ...