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Citations for GE Life Sciences :
1451 - 1500 of 2256 citations for AMPK alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... and filtered onto 1 μm polycarbonate membrane filters (142 mm diameter, Whatman/GE Healthcare, Uppsala, Sweden) representing the viral fraction ...
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bioRxiv - Immunology 2022Quote: ... Th gB-1 mRNA was purified by fast protein liquid chromatography (FPLC; Akta purifier; GE Healthcare)79 ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Biochemistry 2023Quote: ... the solubilized enzymes were loaded onto a 1 mL Q-sepharose HP column (GE Healthcare, UK) pre-equilibrated in buffer containing 20[mM Tris-HCl (pH 7.14 at 20[°C) ...
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bioRxiv - Cell Biology 2022Quote: ... and cloned into BamHI and NotI restriction sites within the pGEX-4T-1 plasmid (GE Healthcare), such that the gene was in frame with an N-terminal GST tag (pGEX-4T-1-GST-KIN-EC-ter) ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted sample was then applied to a 1 mL HiTrap Q HP column (GE Healthcare), and UBXD1 was eluted with a 0-50% gradient of AEX elution buffer (AEX binding buffer supplemented with 1000 mM KCl) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered (Filter paper, Grade 1; Size: 185 mm; Whatman, GE Healthcare Live Sciences), a 1 mL aliquot of the filtrate was transferred into a 1.5 mL centrifuge tube ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cpxA31-163 was cloned into BamHI/EcoRI-digested pGEX-6P-1 expression vector (GE Healthcare), which encodes for an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on a 1 ml HisTrap HP Q column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and applied to a Superdex 200 10/300 size exclusion column (GE Healthcare) that was pre-equilibrated with cross-linking buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was applied to 1 ml Nickel resin (Ni Sepharose 6 Fast Flow, GE Healthcare) preequilibrated with 20 ml buffer A and 20 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) and purified with GST-BIN1_SH3 bound to Glutathione-Sepharose 4B beads (GE Healthcare). Human SH3 of BIN1 with GST tag (GST-SH3 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v, GE Healthcare) and vimentin levels were estimated by western blot as described below ...
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bioRxiv - Genomics 2024Quote: ... SERBP1 immunoreactivity was detected with a horseradish peroxidase– conjugated anti-mouse IgG (1:5000, GE Healthcare). GAPDH immunoreactivity was detected using an anti-mouse IgG (1:6000 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) was exchanged to a storage buffer omitting DTT using a PD10 (GE Healthcare) gel filtration column ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with Anti-mouse HPR-conjugated secondary antibody (1:5000 dilution, GE Healthcare) at RT for 2 hours and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2020Quote: ... The PuACO1 or PuERF2 CDSs were inserted into the pGEX4T-1 vector (GE Healthcare, http://www3.gehealthcare.com) downstream from GST ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNA encoding mouse TRIM41 (residue 35-85 aa, NP_663352.2) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Cell Biology 2020Quote: ... peroxidase-conjugated sheep anti-mouse IgG antibody diluted in 1:4000 (NA931, GE Healthcare, Little Chalfont, UK); and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934 ...
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bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol) and further purified by gel filtration over a Superdex-200 column (GE Healthcare). The protein product was concentrated with a centrifugal filter unit (MD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Biophysics 2020Quote: ... the sample supernatant fraction was incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, 17-0756-01) for 3 h at 4 °C to bind GST-tagged proteins ...
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bioRxiv - Bioengineering 2019Quote: ... Whatman Grade 1 chromatography paper for the paper-based reactions was obtained from GE Healthcare (Mumbai, India). DNA ladder (O’GeneRuler ultra low range 10–300 bp ladder) ...
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bioRxiv - Molecular Biology 2020Quote: ... 12.5% acrylamide:bis-acrylamide 19:1) electrophoresis and heat/vacuum dried for autoradiography (Amersham Hyperfilm ECL, GE Healthcare). Images were analyzed using ImageJ ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM β-mercaptoethanol and loaded onto a 1 ml MonoQ anion exchange chromatography column (GE Healthcare). After washing with 10 column volumes of buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HRP conjugated secondary antibodies were used at 1:10,000 and visualised using ECL (GE Healthcare, Buckinghamshire, UK), high performance chemiluminescence film (Amersham Hyperfilm ECL ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK). Ban quantification was done using Image LabTM v ...
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bioRxiv - Biochemistry 2021Quote: Calorimetric titration of Mint3NT with FIH-1 was performed using a MicroCal™ iTC200 system (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) was subcloned into the BamHI and XhoI sites of pGEX-4T-1 vector (GE Healthcare, Inc.) using standard PCR-based protocols ...
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bioRxiv - Biochemistry 2020Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA (pH 8.0)) and further purified on a Source 15Q anion exchange column (GE Healthcare) with the application of 50 mL buffer I (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µM all samples) separately was loaded (300 µl) in a superdex-200 increase column (GE Healthcare) attached to a Malvern Panalytical Omnisec resolve-reveal system ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated for 1 min with HRP substrate (ECL Prime Western Blotting Detection Reagent (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo-Fisher-Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 1 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare, 17096902) for POT1 or 1 mL amylose beads (New England BioLabs ...
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bioRxiv - Physiology 2020Quote: ... All membranes were incubated with secondary horseradish peroxidase conjugated antibody (1:10000; GE Healthcare, Chicago, Illinois, USA) for 1-2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK). Band quantification was done using Image LabTM v ...
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bioRxiv - Cancer Biology 2022Quote: ... WT and mutant Ankrd26 N-Ank modules were generated by cloning into pGEX-6P-1 (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... generated by cloning of corresponding ORFs between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare), expressed PhoPN ...