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Citations for GE Life Sciences :
1501 - 1550 of 2256 citations for AMPK alpha 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were supplemented with 1 mM DTT and incubated with GSH-Sepharose 4B matrix (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Samples seeded on #1 thickness coverslips were imaged on a DeltaVision Core light microscopy system (GE Healthcare) using either a 60x/1.35na or 100x/1.4na objective fitted to an Olympus IX-71 microscope stand ...
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bioRxiv - Cell Biology 2019Quote: ... Siz1 (167-508) or Siz2 (130-490) was purified from MBPTrap HP 1 mL column (GE Healthcare) with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... FLAG eluate was loaded onto a 1 mL HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic bump and washed with 5 column volumes of 26S NiA buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoblots were washed with TBS-T and incubated with HRP-conjugated secondary antibodies (GE Healthcare, 1:5,000) for one hour in room temperature before development in films ...
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bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Plant Biology 2021Quote: ... and secondary horseradish peroxidase-conjugated antibodies 1:10 000 in PBS 0.1% Tween-20 (Amersham, GE Healthcare). The immunoreaction was developed using the ECL Prime kit (Amersham ...
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bioRxiv - Biochemistry 2021Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Genetics 2021Quote: ... incubated for 1 h at 65°C and dot-blotted on Amersham HybondTM-XL membrane (GE Healthcare). DNA was fixed by UV using a UV crosslinker BLX-254 (Analytik Jena) ...
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bioRxiv - Microbiology 2020Quote: ... Eggs were collected on a wet filter-paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK). Eggs were desiccated for 5 days and later hatched in deionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... 0.03 % Triton-X 100) and proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Molecular Biology 2021Quote: ... and then detected with sheep anti-mouse IgG-HRP (GE Healthcare, Little Chalfont, UK; NA931V, 1:5,000), and donkey anti-rabbit IgG-HRP (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT and further purified by ionic exchange chromatography using a Mono-Q column (GE Healthcare). Protein was eluted with a linear gradient of a buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded onto an immobilized metal-affinity column (1 mL, HIStrap HP column, GE Healthcare), which was washed with 50 mM sodium phosphate (NaPi ...
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bioRxiv - Microbiology 2022Quote: ... Eggs were collected on a wet filter-paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK), desiccated for 5 days and later hatched in deionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes incubated with an anti-mouse HRP-linked secondary antibody (1:5000, GE Healthcare, NXA931, RRID:AB_772209). Either Pierce ECL Western Blotting Substrate (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the supernatant was applied to a 1 ml Ni-NTA HisTrap column (GE Healthcare). The protein was eluted with an imidazole gradient using an analogous gradient of HisTrap buffer B (HisTrap buffer A with 500 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies were used together with horseradish peroxidase-conjugated α-mouse immunoglobulin G (1:5000, GE Healthcare) as secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... antibodies were then incubated for 1 hour and revealed with the enhanced ECL detection system (GE Healthcare). The primary antibody against P2X7 (#APR-004 ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was loaded onto a 1 ml StrepTrap HP column (GE Healthcare Life Sciences, Buckinghamshire, UK) that was equilibrated with Lysis buffer.
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bioRxiv - Microbiology 2023Quote: ... were used together with horseradish peroxidase-conjugated sheep α-mouse immunoglobulin G (dilution: 1:2,000) (GE Healthcare) as a secondary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse IgG (GE Healthcare, NA931VS; 1/10000) and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 min and imaged using the chemiluminescence setting on an Amersham 680 gel imager (GE Healthcare). Blots were adjusted for brightness and contrast using GIMP (GNU Project ...
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bioRxiv - Microbiology 2024Quote: ... were used together with horseradish peroxidase-conjugated sheep α-mouse immunoglobulin G (dilution: 1:2000) (GE Healthcare) as a secondary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) and separated at 0.25 ml min-1 using an AKTA Pure instrument (GE Healthcare). One ml fractions were collected ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: ... His-TWF-1 was further purified with Superdex-75 increase 10/300 GL gel filtration column (GE Healthcare) equilibrated 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies were incubated for 1 hour at RT and signals were detected with ECL Select (GE Healthcare) and Odyssey Fc (Li-Cor) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Molecular Biology 2020Quote: ... it was passed through Whatman™ Grade 1 filter paper (diameter 150 mm) (GE Healthcare Life Sciences, Ireland), followed by 0.45-μm membrane filtration and then 0.22-μm membrane filtration (ThermoFisher Scientific Ltd) ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were detected with horseradish peroxidase-conjugated secondary antibodies (1:5000, GE Healthcare Life Sciences, #NA931-1ML (Mouse) or #NA934-1ML (Rabbit) ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant corresponding to His-JMJD6 (1-343) was loaded to 7mL of Ni-NTA resin (GE Healthcare), washed with nickel-binding buffer containing 20mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated DJ-1/Hsp32 was loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare), pre-equilibrated with 50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymes were purified from the cell lysate using a 1 ml His-Trap FF crude column (GE Healthcare) using an elution gradient from 10 to 250 mM imidazole in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were solubilized in 1% (w/v) LMNG and proteins were purified using a StrepTrap column (GE Healthcare). The resin was washed in TBS containing 0.01% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL of plasma was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...