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Citations for GE Life Sciences :
1501 - 1550 of 1691 citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed three times with 0.1% PBS-T and were incubated with secondary horseradish peroxidase-coupled antibodies for 1h at RT (GE Healthcare, Jackson ImmunoResearch, 1:10000). ECL Western Blotting Detection Reagent (RPN2106 ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were washed (3x) with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, dilution 1:10.000) or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Physiology 2023Quote: ... with TBS-T and incubated (1-hour, room temperature) with HRP-linked anti-rabbit secondary antibody (1:10,000; GE Healthcare Bioscience, Baie d’Urfe, Canada). Chemiluminescence was assessed using the Luminata™ Crescendo Western HRP Substrate (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) and the protein concentration was determined using a 2-D Quant kit (GE Healthcare, Piscataway NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... The western blot was developed using Amersham ECL kit Start or Prime (Cat# RPN3243 or # RPN2232, GE life sciences).
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bioRxiv - Bioengineering 2019Quote: ... cDNA was synthesized from the purified RNA (kit Ready-To-Go You-Prime First-Strand Beads (GE Healthcare®)).The synthesized cDNA ...
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bioRxiv - Physiology 2019Quote: ... diluted 1:5,000 and developed using Amersham ECL Western Blotting Detection Kit system (GE Healthcare Life Sciences, PA, USA).
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bioRxiv - Biochemistry 2020Quote: ... the Superdex 200 column was calibrated using a gel filtration HMW calibration kit (GE Healthcare Life Science, Cat# 28403842) that included six reference proteins with known molecular weights (MWs ...
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bioRxiv - Plant Biology 2021Quote: Total and virion DNA were amplified by rolling circle amplification (RCA) using the templiPhi100 DNA Amplification Kit (GE Healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... CM5 chip was first coated with streptavidin by standard amine coupling using an amine coupling kit (Biacore, GE Healthcare), followed by immobilization of biotin-heparin (38) ...
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bioRxiv - Biochemistry 2021Quote: ... The signal produced by reaction between HRP and ECL (Kit ECL Plus Western Blotting Detection System, GE Healthcare®) was detected by chemiluminescence using imaging Chemidoc (Biorad®).
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bioRxiv - Microbiology 2019Quote: ... In Study 2 the DNA concentrations were equalised prior to amplification using an Illustra GenomiPhi V2 kit (GE Healthcare). Amplifications of purified viral DNA was performed in triplicate on all samples as described by the manufacturer ...
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bioRxiv - Biophysics 2019Quote: ... We selected four proteins as molecular mass standards from the gel filtration calibration kit HMW (GE Healthcare, Uppsala, Sweden), i.e ...
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bioRxiv - Cancer Biology 2019Quote: STAT3 siRNA knockdown utilized the ON-TARGETplus Human STAT3 siRNA kit from GEHealthcare (L-003544-00-0005, GE Healthcare). This SMARTpool siRNA contains four pooled siRNAs ...
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bioRxiv - Biochemistry 2019Quote: ... The size-exclusion column was calibrated using a set of standard proteins (Gel Filtration HMW Calibration Kit, GE Healthcare) in SEC buffer ...
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bioRxiv - Microbiology 2020Quote: ... pH 4) was covalently immobilized on a CM5 sensor chip using an Amine coupling kit (GE Healthcare Life Sciences). Binding analyses were performed in HBS-P buffer (0.01 M HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Circular viral DNA was amplified using rolling circle amplification (RCA) with Illustra TempliPhi 100 amplification kit (GE Healthcare, USA). The RCA products were used to prepare a 350 bp insert DNA library for each sample following the manufacturer’s standard protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and purified using the Illustra GFXTM PCR DNA and Gel Band purification kit (GE Healthcare, Little Chalfont, Buckinghamshire, UK). The purified PCR products were directly sequenced in both directions ...
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bioRxiv - Genetics 2020Quote: ... whole genome amplification was carried out using an Illustra-GenomiPhi™ V2 DNA Amplification Kit (GE Healthcare, Uppsala, Sweden) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... and purified after agarose gel electrophoresis using the GFX™ PCR DNA and Gel Band Purification Kit (GE Healthcare). Insert fragments were amplified with Q5 DNA polymerase (NEB ...
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bioRxiv - Neuroscience 2022Quote: Total RNA of ARPE-19 cell lines was extracted using the Illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The isolated protist cells were subjected to whole-genome amplification (WGA) using the Illustra GenomiPhi V2 Kit (GE Healthcare) as described previously [37] ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA extraction from bulk tumour or metastases samples was performed using the Illustra RNAspin Mini isolation kit (GE healthcare), following manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was suspended in 50 µl of the hydration solution using a Tissue DNA isolation kit (GE HealthCare LifeSciences).
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cell samples was extracted and subjected to DNaseI treatment using the Illustra RNAspin kit (GE Healthcare). cDNA synthesis from the RNA samples was performed using the High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor (Applied Biosystems #4374966 ...
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bioRxiv - Synthetic Biology 2024Quote: ... cloned plasmids were verified by Sanger sequencing using template generated by the TempliPhi 500 Amplification Kit (GE Life Sciences) according to the manufacturers protocol or by Nanopore sequencing of purified plasmid DNA (Primordium Labs) ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed as previously described using Horseradish Peroxidase (HRP)-conjugated secondary antibody followed by addition of ECL (GE Healthcare Bio-Sciences Corp., USA) to reveal chemiluminescence71 ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG, GE Healthcare Life Sciences; Freiburg, Germany) diluted 1:20,000 in TBS-Tw + 1% milk ...
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... and class II molecules were isolated by standard immunoaffinity purification using purified monoclonal antibody 2G11 [65] produced in-house and covalently linked to CNBr-activated Sepharose (GE Healthcare, Chalfont St Giles, UK). MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed with TBS-T and probed with the species-matching secondary horseradish peroxidase-conjugated antibody: donkey anti-rabbit (GE Healthcare, cat. #NA932, dilution 1:10,000), or sheep anti-mouse IgG (GE Healthcare ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were washed three times with TBST for 10 minutes each and exposed to donkey anti-rabbit secondary antibody conjugated to horse radish peroxidase (GE Healthcare; NA934V; LOT: 16976257; 1:10,000) in TBST containing 5% (w/v ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Genetics 2020Quote: ... Radiolabeled probes were prepared by random priming of PCR products covering the regions of interest with Megaprime kit (GE Healthcare) in the presence of α-32P dCTP (3000 Ci/mmol) ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were developed using the SuperSignal West Femto kit (Pierce) or fluorescence imaging according to the manufacturer’s protocol with Amersham Imager 600 (GE Healthcare).
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bioRxiv - Genomics 2019Quote: ... The plate and column pools were amplified using the Illustra GenomiPhi HY DNA Amplification Kit (GE Healthcare Life Sciences, UK) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Genomics 2020Quote: The total genomic DNA was extracted from a single aphid using Illustra Nucleon Phytopure kit modifying the manufacturer’s protocol (GE Healthcare). The lysis step was performed adding 10 ul of Proteinase K and incubating the sample in a water bath for 2 hours at 55°C ...