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Citations for GE Life Sciences :
1151 - 1200 of 1645 citations for SpectraDye Antibody Labeling Kit IR700 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The protein concentration was measured using 2-D Quant Kit (GE Healthcare, Piscataway, NJ, USA).
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bioRxiv - Neuroscience 2020Quote: Protein concentration in sarkosyl extracted samples was determined using the 2D-Quant Kit (GE Healthcare) following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX ...
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bioRxiv - Genetics 2020Quote: ... RNA was reverse-transcribed to cDNA using the First-Strand cDNA Synthesis Kit (GE Healthcare) following manufacturer’s guidelines and was stored at −20°C.
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bioRxiv - Microbiology 2021Quote: ... and total RNA extraction was performed using Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare). P ...
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bioRxiv - Microbiology 2021Quote: ... and total RNA was extracted using the Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Immunodetection was performed using the Amersham ECL Plus kit (GE Healthcare, Little Chalfont, Buckinghamshire, UK). Primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... Standard proteins used were from the gel filtration calibration kit LMW and HMW (GE Healthcare) with the exception of Cryptococcus neoformans acyl-CoA binding protein (ACBP ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized with an ECL western blotting detection kit (GE Healthcare, Chicago, IL, USA) through an Azure c600 gel imaging system and bands were quantified with an Azurespot software (Dublin ...
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bioRxiv - Immunology 2021Quote: ... and mRNA was transcribed into cDNA using the First-Strand cDNA Synthesis Kit (GE Healthcare). The Nb repertoire was isolated in 3 subsequent PCR reactions using the following primer combinations (1 ...
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bioRxiv - Genomics 2022Quote: ... Rolling circle amplification was then performed using the Illustra TempliPhi 100 Amplification Kit (GE Healthcare). Precipitated DNA was resuspended directly in 20 µl of the Illustra TempliPhi sample buffer and the amplification reaction was allowed to proceed for 24 hours at 30°C.
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bioRxiv - Biochemistry 2022Quote: ... and purified by Illustra GFX PCR DNA and a gel band purification kit (GE Healthcare). DNA sequencing was accomplished using 3130xl Genetic Analyzer (Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare). Restriction endonucleases and T4 DNA ligase were purchased from Thermo Fisher Scientific and PrimeSTAR DNA polymerase from Takara Bio ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total RNA was isolated using the Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare, UK), and reverse transcription was carried out using the Improm-II reverse transcriptase (Promega ...
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bioRxiv - Plant Biology 2022Quote: Method B) Genomic DNA was extracted using the Nucleon Phytopure DNA extraction kit (GE Healthcare Gio-Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... Upon visualization by using the ECL Advanced Chemiluminescence kit (GE Healthcare Life Sciences®, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from the tumor tissue using an RNAspin Mini kit (GE Healthcare, UK). After passing the quality check ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were revealed with the ECL-plus chemiluminescence western blot kit (Amersham-GE Healthcare, UK).
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bioRxiv - Cancer Biology 2023Quote: Cellular RNA was extracted using RNAspin Mini RNA Isolation Kit (GE Healthcare; #25-0500-71) and cDNA was reverse transcribed using iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... at room temperature for 1 hour before visualization using the ECL Detection kit (GE Healthcare).
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bioRxiv - Molecular Biology 2024Quote: ... The column was calibrated using the gel filtration calibration kit LMW and HMW (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The reaction was visualized by chemiluminescence using ECL Advance Western Blotting Detection kit (GE HealthCare) and the dots quantified using ImageJ NIH software (version 1.50i).
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and incubated in 50 μL per well of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) or goat anti-hamster IgG-HRP conjugated antibody (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were washed 3 times with PBST and 50 µL of sheep anti-mouse IgG-horseradish peroxidase (HRP) conjugated antibody (GE Healthcare) was added at a dilution of 1:3,000 in blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were visualized using a horseradish peroxidase-conjugated secondary antibody followed by enhanced chemiluminescence detection with an ImageQuant LAS 4000 developer (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: The binding affinity of the discovered antibodies for α-cobratoxin was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). All measurements were performed at 25 °C using 10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: Kinetic interactions between the antibodies and his-tagged antigen proteins were measured at room temperature using Biacore T200 surface plasmon resonance (GE Healthcare). Anti-human fragment crystallizable region (Fc region ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... S9.6 antibodies were purified according to standard procedures (Stork et al., 2016) by using a HiTrap Protein G HP column (GE Healthcare), followed by extensive washing with 20 column volumes of PBS and elution with five column volumes of elution buffer (0.1 M glycine-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... the strips were washed with TBS and incubated with anti-rabbit IgG HRP-conjugated antibodies (RRID: AB_772206, GE Healthcare, Pittsburgh, PA) at a 1:20,000 dilution in TBS + 3% BSA ...
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bioRxiv - Biochemistry 2019Quote: ... and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3, GE Healthcare). Detection was carried out with the Ettan DIGE Imager (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... washed 3x with TBS-T and incubated for 1 hours at RT with the secondary antibody (horseradish peroxidase-conjugated sheep anti-mouse or donkey anti-rabbit IgG, GE Healthcare). Membranes were analyzed with a Fusion Solo (Vilber Lourmat) ...
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bioRxiv - Biophysics 2019Quote: ... The remaining lysate was then incubated overnight at 4°C with anti-GFP (Rudner et al., 1999) or anti-SMC (Lindow et al., 2002) antibodies and subsequently incubated by Protein A-Sepharose resin (GE HealthCare) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... plaque assays were washed with PBS three times and then incubated for 1 h in anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-tubulin antibody (1:1000, DSHB, University of Iowa, Iowa City, IA, USA) and secondary anti-mouse IgG-HRP (1:2000, GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies were diluted in Can Get Signal Immunoreaction Enhancer Solution (Toyobo, NKB-101) and detected with ECL Prime (GE Healthcare, #RPN2232).
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bioRxiv - Genetics 2019Quote: ... Following washing with TBST membranes were incubated with ECL horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare, Chicago, IL, USA) diluted 1:5000 in blocking buffer for one hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by the incubation with anti-rabbit or anti-mouse IgG antibody conjugated to horseradish peroxidase (NA934V or NA931V, GE Healthcare). After reactivation with Chemi-Lumi One Ultra (nacalai tesque) ...
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bioRxiv - Microbiology 2021Quote: ... The expressed GP was purified using an mAB100 antibody column followed by SEC on a HiLoad Superdex 200 16/600 column (GE Healthcare). PBS (pH 7.2 ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody expression vectors were co-transfected into 293F cells and the cell culture supernatants containing secreted antibody IgG were purified by columns packed in-house containing Protein A Sepharose beads (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... and detection of enhanced chemilluminescence from HRP-coupled anti-rabbit secondary antibodies followed by detection with an ImageQuant LAS4000 mini imaging system and analysis with ImageQuant software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... and then detected with an anti-rabbit horseradish peroxidase-linked secondary antibody using an ImageQuant LAS 4000 mini digital imaging system (GE Healthcare). Detection of DnaA ...
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bioRxiv - Biochemistry 2020Quote: ... YL½ antibody (IgG, expressed from hybridoma cells and purified by ion exchange chromatography) was coupled to CNBr-activated sepharose beads (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated for 1 h with 5 μL of anti-MYC antibody (Gentex) followed by 1 h incubation with an additional 50 μL of GammaBind plus Sepharose beads (GE Healthcare) to capture protein complexes ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Systems Biology 2020Quote: ... the target-specific antibody was added to the cellular lysates together with 25 μl of Protein A or G sepharose (GE Healthcare) depending on the species of target antibody and the mixture was incubated overnight rotating at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Membranes were washed 3 times with TBS-T and stained with 1:10 000 HRP-coupled anti-rabbit (anti-Rabbit IgG, HRP linked antibody from donkey, GE HealthCare) in blocking buffer for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).