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Citations for GE Life Sciences :
1401 - 1450 of 2196 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1878 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The microfluidic Y04E plate was then loaded into an environmental chamber heated to 30°C mounted on a DeltaVision Elite wide-field fluorescence microscope (GE Healthcare) with a PCO Edge sCMOS camera and operated by the associated softWoRx software ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant of the cell lysate was incubated overnight at 4°C on a wheel with Glutathione Sepharose 4B resin (GSTrap 4B, GE Healthcare). After several washes ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fractions were removed by centrifugation at 20000 g during 30 min at 4°C and the supernatant was loaded on a 1 mL HisTrapTM HP column (GE Healthcare) connected to a NGC chromatography system (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 4 °C overnight as described above and scanned with GE Amersham Typhoon-5 fluorescence gel and blot scanner (GE Healthcare) with red laser.
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bioRxiv - Biochemistry 2023Quote: ... the N-terminal His tag was cleaved by overnight dialysis with TEV protease at 4 °C and purified by size exclusion chromatography (SEC) (HiLoad Superdex-200 16/600 column, GE Healthcare) in CHIP storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... actin Ac-74 or Pol II S5P) overnight at +4°C and immune-complexes were collected with 50 μl of protein A sepharose (GE Healthcare) at +4°C for two hours on rotation ...
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bioRxiv - Neuroscience 2023Quote: ... coupled with the epi-fluorescence illuminator Nikon Intensilight C-HGFIE and viral particles were purified with heparin (GE Healthcare; Cat.nr. 170406013). The concentration of the virus was checked with qPCR (fwd ITR primer ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Labelling reactions proceeded for 40 mins at 4°C and the excess dye was removed using NAP-5 desalting columns (17-0853-01, GE Healthcare). Nanobodies to be used for confocal microscopy were labelled with Alexa488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... hDrp1 was eluted by cleavage from the affinity resin using 30 mM DTT at pH 8 for 48 hours at 4 °C and further purified using anion exchange chromatography (HiTrap Q FF, GE Healthcare) at pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with a linear gradient of increasing NaCl concentration from 300 mM to 1 M in 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated first with primary Abs for 24 hours at 4°C and then with HRP–conjugated secondary Abs (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the protein debris was removed by centrifugation (30000×g, 20 min at 4 °C) and the supernatants were applied to His-Trap chelating column (GE Healthcare), washed by refolding buffer and eluted with the same buffer containing 30 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... the ∼150 mM potassium phosphate buffer of the bPKA-C stock was exchanged to 10 mM potassium phosphate using a Hi-TRAP desalting column (GE Healthcare). Thus ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: All TIRF experiments (except Figure 3 F and G) were acquired at 37 °C/5% CO2 with the DeltaVision OMX SR microscope (GE Healthcare) with a 60×/1.42 NA oil Plan Apochromat objective (Olympus ...
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bioRxiv - Genetics 2024Quote: ... and 5% dextran sulfate) at 65°C probed with 32P-labeled V5 DNA probes prepared via Amersham Megaprime DNA labeling kit (GE Healthcare) and Illustra ProbeQuant columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies pre-adsorbed to protein G-sepharose beads (GE Healthcare) were added to the cell extract and the mixture incubated for 2 hr at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated with corresponding horseradish peroxidase-linked secondary antibodies (Amersham, GE Healthcare) for 1h at room temperature and detected by chemiluminescence imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... incubated with appropriate HRP-labelled secondary antibodies (GE Healthcare Life Sciences), washed as before and developed using ECL (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... The antibody was detected using the ECL plus reagents (GE Healthcare). Detection was done in a dark room ...
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bioRxiv - Immunology 2021Quote: ... for IgG antibody or LambdaFabSelect Agarose Beads (GE Healthcare Life Sciences) for Fab at 4°C on a rotating shaker overnight ...
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bioRxiv - Immunology 2021Quote: ... Plasma antibodies were purified using Protein A Sepharose resin (GE Healthcare), eluted from the resin with 0.1 M glycine at pH 2.5 and buffer exchanged into 1X PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HRP-coupled antibodies were detected by enhanced chemiluminescence (Amersham, GE Healthcare) and X-ray films ...
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bioRxiv - Immunology 2022Quote: ... The undigested antibodies were removed with Protein A Sepharose (GE Healthcare) and the flow through was collected ...
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bioRxiv - Developmental Biology 2019Quote: ... After incubation with HRP-conjugated secondary antibodies (GE Healthcare, 1:5000), signals were detected using SuperSignal™ Femto West Chemoluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were purified via HiTrap Protein A HP columns (GE Healthcare) run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibody was visualized using ECL chemiluminescent detection reagent (GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... using native Msh5 antibody and Protein A Sepharose beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... Antibody complexes were detected using enhanced chemiluminescence detection reagents (GE Healthcare) and digital images captured using GeneSnap (SynGene ...
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bioRxiv - Immunology 2020Quote: ... Purification of labelled antibodies was performed using NAP5 Columns (GE HealthCare). The dye to antibody ratio was quantified using Nanodrop and only antibodies with a composition of 3-4 Alexa Fluor 405 and 0.9-1.2 Alexa Fluor 647 per antibody were used for imaging.
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bioRxiv - Immunology 2021Quote: ... Hit detected by fluorescent secondary antibody using ImageQuant software (GE Healthcare). An expression vector (plRES-hEGFR- IRES-ZsGreen1 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were analyzed by size exclusion chromatography (SEC) (Cytiva, GE Healthcare) and separated for monomeric fraction in case aggregation levels were >5% ...
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bioRxiv - Genetics 2020Quote: ... the membranes were incubated with HRP-conjugated secondary antibodies (GE Healthcare) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies and signal was detected using ECL Plus (GE Healthcare). Blots were stripped and re-probed with anti-actin antibody (Santa Cruz).
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bioRxiv - Cancer Biology 2022Quote: ... Bound antibodies were detected using chemiluminescence (ECL, GE Healthcare, Cat# GERPN2232) and imaged using the Fusion Fx Imaging System (Vilber ...
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bioRxiv - Microbiology 2022Quote: ... Bound antibodies were detected with ECL Plus Detection substrate (GE Healthcare) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Plant Biology 2022Quote: ... Secondary antibodies were horseradish peroxidase-conjugated (GE Healthcare; NA934 and NA931).
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bioRxiv - Microbiology 2022Quote: ... horse radish peroxidase (HRP)[conjugated secondary antibodies (GE Healthcare Life Sciences) were diluted 1:2,000 in blocking buffer and incubated (1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoblots were developed using horseradish peroxidase-conjugated secondary antibodies (GE Healthcare) and the Western Lightning® Plus-ECL reagent (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with corresponding horseradish peroxidase-linked secondary antibodies (Amersham, GE Healthcare) for 1hour at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated with horseradish peroxidase-linked secondary antibody (GE Healthcare) and visualized using the ECL pico plus chemiluminescence method (Pierce ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody (6A3) was immobilised on a CM5 biosensor chip (GE Healthcare) at 10–20 μg/mL concentration in 10 mM acetate solution (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... and revealed with appropriate secondary antibodies (GE Healthcare or Sigma, A9044) and the ECL 2 substrate (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies were purchased from GE Healthcare (Japan) (1:1000) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by HRP-conjugated rabbit secondary antibody (1:50,000, GE Healthcare) for 1 h at room temperature and the signals were visualized using chemiluminescence reagents (Thermo Scientific).