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Citations for GE Life Sciences :
1351 - 1400 of 2196 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1878 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Aβ42 peptides in the filtrate were further concentrated to ∼50 μM at 4°C and 4000×g with a 5 000 Da Vivaspin concentrator (GE Healthcare). The Aβ42 peptide concentration was calculated using an extinction coefficient of 1400 M-1cm-1 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mg of total protein was pre-cleared for 3h at 4°C by incubation with glutathione-coupled sepharose beads (GE Healthcare) previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Aggregates were removed by centrifugation (20 min, 48000xg, 4° C) and the protein solution loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated in 20 mM MOPS-NaOH pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... proteins were concentrated by ultrafiltration at 4 °C (MWCO: 10 kDa) and passed over a Superdex 75 26/60 gel filtration column (GE Healthcare) equilibrated in 20 mM sodium phosphate pH 7.4 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 4 °C) and the supernatant was incubated with 75 μl of IgG Sepharose 6 Fastbeads (GE Healthcare, 52-2083-00 AH) by rotating at 4 °C for 2 to 3 hours to enable binding of the tagged protein ...
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bioRxiv - Microbiology 2022Quote: ... Size exclusion chromatography was performed in 20 °C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Microbiology 2022Quote: ... The N- and C-terminal domains were separated by size exclusion chromatography using HiLoad 16/600 Superdex 200 column (GE Healthcare) in AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The lysates were clarified by centrifugation at 20 000 × g for 1 hour at 4 °C before loading on a 5 mL HiTrap FF Ni-NTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate was centrifuged at 15557 g for 30 min at 4°C and the supernatant was loaded on a HisTrap column (GE Healthcare) equilibrated in 20 mM HEPES 150 mM NaCl 20 mM imidazole 0.02% NaN3 pH = 7.4) ...
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bioRxiv - Plant Biology 2022Quote: ... Purification tags were then removed by incubating with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... oryzae isolates were maintained on Potato Dextrose Agar medium at 24°C and as frozen stocks of dried mycelium on Whatman filter paper (GE Healthcare Whatman™ Qualitative Filter Paper ...
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bioRxiv - Microbiology 2022Quote: ... The sample was incubated at 4°C for 30 minutes and further purified using a HiLoad 26/600 Superdex 200 (GE healthcare).
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bioRxiv - Plant Biology 2022Quote: ... coli strain BL21 with protein induction when OD600 reached 0.5 with 1 mM IPTG (isopropyl b-D-1-Thiogalactopyranoside) for 4 h at 37°C and then purified over a glutathione Sepharose 4B column (GE Healthcare). The eluted samples were then resolved by SDS-PAGE and stained by Coomassie brilliant blue R-250 to evaluate purity ...
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bioRxiv - Molecular Biology 2022Quote: ... the pre-cleaning step was omitted and the ChIP extract directly incubated o/n on a rotor at 4°C with 50 μl IgG Sepharose 6 Fast Flow beads (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... After sonication the lysate was clarified by centrifugation (22 500 rpm/61 236 x g, 20 min, 4 °C) and applied to a HisTrap FF column (GE Healthcare) equilibrated with 10 column volumes (CV ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were filtered and then pelleted at 300 g for 5 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Biophysics 2024Quote: ... The peak elution fractions were pooled and concentrated to 2 mL using a Amicon Ultra 10K MWCO centrifugal filter at 4 °C and was loaded into a HiLoad Superdex 200 16/60 column (GE healthcare) equilibrated with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2024Quote: ... The sample was centrifuged (10 min, 20000g, 4 °C) and applied to Superdex 200 10/300 gel-filtration column (GE Healthcare) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... SC and the D1 domain of FcμR was first immobilized on the chip: SC was biotinylated at the C-terminal and immobilized on the SA chip (GE Healthcare), whereas FcμR-D1 was immobilized on the CM5 chip to a level of resonance units similar to pIgR ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged sample was lastly purified by size exclusion chromatography (SEC) at 4 °C by injecting sample onto a Superose 6 Increase 10/ 300 GL column (GE Healthcare) equilibrated in SEC buffer (50 mM Tris ...
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bioRxiv - Biophysics 2024Quote: All SPR binding experiments were performed on a CM5 chip (Cytiva) at 25 °C using a Biacore T200 Instrument (GE Healthcare). The purified CNBH domains were immobilized on the CM5 chip using a standard amine coupling chemistry in the presence of 10 mM sodium acetate buffer at pH 5.5 as the immobilization buffer (buffer used to directly dissolve ligands) ...
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bioRxiv - Microbiology 2023Quote: ... After 2.5 days the diatom cultures were filtered (1.2 µm pore size, GF/C, Whatman, GE Healthcare, Little Chalfont, United Kingdom). The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30 min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (PBS 1X ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 1h at 20000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged protein was eluted with a linear gradient of buffer B (50 mM Hepes pH 7 ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was centrifuged for 30min at 30000 x g at 4°C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). The His-tagged protein was eluted with a linear gradient of buffer B (Hepes 50 mM pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... Gels were blot-transferred to a nylon membrane and probed at 60°C with the YCp1.3 DNA labelled with AlkPhos Direct (GE Healthcare®). Chemiluminescent signals of increasing exposure periods were recorded on X-ray films ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were filtered and then pelleted at 300 g for 10 minutes at 4°C before being resuspended in 22% Percoll (GE Healthcare) and centrifuged at 900 g for 20 minutes with acceleration set to 4 and deceleration set to 1 in order to remove cellular debris ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% dextran sulfate) at 65°C probed with 32P-labeled CLB3 DNA probes prepared via Amersham Megaprime DNA labeling kit (GE Healthcare) and Illustra ProbeQuant columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained BED were concentrated and further purified at 4 °C on a preparative Superdex 75 16/600 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions that contained the protein were concentrated and further purified at 4 °C on a preparative Superose 6 XK 16/70 size exclusion column (GE Healthcare) equilibrated with 25 mM HEPES.Na pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... All chromatography procedures were carried out at 4°C using either an ÄKTA express or ÄKTA pure chromatography system (GE Healthcare). 1 mL StrepTrapTM HP (cat# 28907546 ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were centrifuged at 4°C to remove cell debris and the supernatant was filtered and loaded onto a Nickel Sepharose Fast Flow column (GE Healthcare) in the case of pSY10 constructs ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble fraction was removed by centrifugation at 75,000g for 30 min at 4°C and the supernatant was applied to NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The mixed complexes were incubated over night at 4 °C and the formed R2SP complex was separated from excess SPAG1/PIH1D2 using a Superose 6 16/60 XK (GE Healthcare), see Supplementary Information for detailed protocol ...
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bioRxiv - Biophysics 2023Quote: ... 3,000 g × 15 min at 4 °C) and further purified by size-exclusion chromatography (ÄKTA pure system, Superdex-75 Increase 10/300 GL, GE Healthcare). The unfolding and refolding process for GlnBP WT was conducted under the same conditions as described for the GlnBP variants.
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was clarified by centrifugation at 13000 rpm for 10 min at 4°C by a table-top centrifuge and further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in chromatography buffer (20 mM HEPES–KOH pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Images were acquired with 200 ms exposure time every 6 minutes for at least 6 h at 30°C using Softworx (Applied Precision) software ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the soluble proteins with His6 tag were purified on 1ml of nickel phosphate loaded sepharose beads (GE Healthcare). Bound proteins were washed in lysis buffer with 25mM imidazole then with 400mM Imidazole ...
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bioRxiv - Biophysics 2022Quote: ... was performed at 20 °C using S75 (IDR constructs) and S200 Increase 5/150 (NTD and ARD constructs) analytical SEC columns (GE Healthcare) equilibrated in the appropriate buffers (see Tables S1 and S2 ...
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bioRxiv - Microbiology 2023Quote: ... Size exclusion chromatography was performed in 20°C ÄKTA purifier system (Amersham) and a prepacked Hi-Prep 16/60 Sephacryl S-200 high-resolution column (GE Healthcare). Flow rate was 0.5 ml/min ...
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bioRxiv - Cancer Biology 2023Quote: ... Recipient mice were allowed to recover for 6 weeks post-transplantation before Cre-LoxP recombination was induced by two intraperitoneal injections of Poly(I)-Poly(C) (300 μg in 200 μl PBS; #27-4732-01 GE Healthcare) with 48 h rest in-between ...