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Citations for GE Life Sciences :
1301 - 1350 of 2196 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1878 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: Size-Exclusion Chromatography-Multi Angle Light Scattering (SEC-MALS) experiment was performed at 25°C using a Superdex 200 10/300 GL column (GE HealthCare) connected to a miniDAWN-TREOS light scattering detector and an Optilab T-rEX differential refractive index detector (Wyatt Technology ...
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bioRxiv - Microbiology 2020Quote: ... amino acids 319-537) (Acro Biosystems, Newark, NJ) protein was measured at 25°C using BIAcore T200 surface plasmon resonance (SPR) (GE Healthcare). STI-1499 or STI-2020 antibody was covalently immobilized on a CM5 sensor chip to approximately 500 and 100 resonance units (RU) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 μm; GE Healthcare). Entry routes were determined by pre-treating monolayers of VeroE6 or VeroE6-TMPRSS2 cells with a concentration range of camostat mesylate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated at 37°C overnight and then scanned on the Amersham Typhoon Biomolecular Imager (channel Cy2; resolution 10 mm; GE Healthcare). All pseudovirus experiments were quantified using ImageQuant TL 8.2 image analysis software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by incubating the purified proteins with ∼3% (w/w) thrombin (4°C, overnight) and then subjected to SEC using a Superdex S-200 16/60 column (GE Healthcare) pre-equilibrated with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: 1.5 mg of pure PfP2 incubated at 37°C for 3h and was subsequently loaded onto a Hi Load Superdex 75 16/600 gel filtration column (GE Healthcare) pre-equilibrated with 20mM Tris-HCl (pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... in complex with tri- and tetra-ubiquitin at 30°C using a Viscotek TDA 305 triple array detector (Malvern Instruments) downstream to an Äkta Purifier (GE Healthcare) equipped with an analytical size exclusion column (Superdex 200 10/300 GL ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE Healthcare Biosciences, 30 min, 4°C, 2,100 r.p.m.). PBMCs were extensively washed in cold phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Concentrated protein was spun down 15 min in a bench-top centrifuge (4°C) and immediately loaded onto a HiLoad 16/600 Superdex 200 column (GE Healthcare), preequilibrated in gel filtration buffer consisting of 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... spun down 15 min at 15000 rpm in a bench-top centrifuge (4°C) and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare), previously equilibrated in gel filtration buffer (30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated in a rotator overnight at 4°C and the immunocomplexes were recovered with FA buffer pre-equilibrated SPA/G beads (GE Healthcare) by rotating 2h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tagged 14-3-3ε was allowed to equilibrate with the excess of untagged 14-3-3γ (overnight at 4 °C) and then loaded on a 1 ml HisTrap HP column (GE Healthcare) while the flowthrough fraction containing 14-3-3γ was collected ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified by centrifugation at 40,000 g for 10 min at 4 °C and the proteins were purified using a 5 ml GSTrap column (GE Healthcare) and subsequent size exclusion chromatography on a Superdex 200 16/60 column vs ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were heat inactivated for 20 min at 65°C and the unincorporated radioactivity was eliminated with a G25 Illustra MicroSpin column (GE Healthcare) according to the manufacture’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Microbiology 2021Quote: Binding of C1 and its sub-components to GST-ErpB and GST-ErpQ was performed at 25°C using a Biacore T200 (GE Healthcare) as previously described (Garcia 2016) ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with 5 μl rabbit polyclonal anti-GM130 serum or 5 μl pre-immune serum for 60 min at 4°C and incubated with protein A-Sepharose (GE Healthcare) for additional 60 min ...
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bioRxiv - Physiology 2021Quote: ... The hemolysate was then centrifuged at 4°C (12000 g, 20 min) to remove cell debris and then passed (‘stripped’) on a PD-10 desalting column (GE Healthcare) equilibrated with 10 mM HEPES ...
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bioRxiv - Immunology 2019Quote: ... SI-LP and LI-LP cells were purified by density gradient centrifugation (600 rcf for 20 min at 22 °C, acceleration 5 and brake 0) with 40/70 % Percoll (GE Healthcare). Cell suspensions were subsequently filtered through 100 µm cell strainers (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated by protein electrophoresis at 120 V for 2 h using 10% Bis-Tris pre-cast gels (NuPage) and transferred overnight at 4°C onto Hybond-P PVDF membranes (GE Healthcare). After blocking in ECL blocking agent (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... experiments were performed at 30°C using a Viscotek TDA 305 triple array detector (Malvern Instruments) downstream to an Äkta Purifier (GE Healthcare) equipped with an analytical size exclusion column (Superdex 75 or 200 10/300 GL ...
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bioRxiv - Biophysics 2019Quote: ... and protein was released from the resin by PreScission Protease (PPX) action at 4 °C overnight and further purified on a Superdex 200 column (GE Healthcare) equilibrated in SEC buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Cell extract was clarified by ultracentrifugation at 140 000 x g at 4 °C for 45 min and recombinant CNOT7 protein was purified automatically using ÄKTAxpress FPLC system (GE Healthcare). The following protocol was used for automated purification ...
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bioRxiv - Biophysics 2019Quote: ... The resulting suspensions were centrifuged (13000 rpm for 35 min at 4°C) and the supernatants were applied to an HisTrap HP column (GE Healthcare) previously equilibrated with loading buffer (20 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... mosaic NP preps were stored for a month at 4°C and then analyzed via SEC using a Superose 6 10/300 (GE Healthcare) column equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 35000 g for 30 minutes at 4° C and the supernatant was loaded onto a pre-equilibrated HiTrap Chelating HP column charged with nickel ions (GE Healthcare). The column was washed extensively with wash buffer (20 mM Tris pH 7.9 ...
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bioRxiv - Genomics 2021Quote: ... Gels were exposed on a phosphorimager screen overnight at -80°C and screens were scanned using a Typhoon scanner (GE healthcare). Images were analysed using the ImageQuant software (GE healthcare).
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Biochemistry 2021Quote: ... spun at max speed for 30 min in a bench-top centrifuge (at 4°C) and finally loaded on a Superdex200 16/60 (GE Healthcare) previously equilibrated in MPS1 buffer (250 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... samples were concentrated in 10K MWCO centricons (PAL Corporation) (5000rpm [3214xg] at 4°C) and applied on a Superdex200 HiLoad 16/600 column (GE Healthcare) using 25 mM Hepes pH 7.6 ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was inactivated at 75 °C for 10 min and the labeled RNA/DNA was purified by gel filtration (Illustra MicroSpin G-25 columns; GE Healthcare). Recombinant ...
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bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C before analysis by SDS-PAGE and transfer onto a nitrocellulose membrane (Protran, 45 µm pores, GE Healthcare). DACone adducts onto BLG were visualized by scanning the membrane with an infrared imager (Odyssey ...
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bioRxiv - Biochemistry 2021Quote: ... which was then used to capture purified HexaPro S2 by the c-terminal twin StrepTag to ~80 response units (RU) in each cycle using a Biacore X100 (GE Healthcare). The binding surface was regenerated between cycles using 0.1% SDS followed by 10 mM Glycine at pH 2 ...
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bioRxiv - Microbiology 2021Quote: ... The nanobodies were fused with a His-tag at C-terminus and purified over Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 400 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... 5 min at 4 °C followed by an anion exchange chromatography loading the supernatant on a HiTrap Q HP 5 ml column (GE Healthcare) and intensive wash step of 20 column volumes with ion exchange buffer A ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Immunology 2021Quote: ... 13,200 rpm at 4°C) and the resulting supernatant was precleared for 30 min using rProtein A Sepharose fast-flow beads (GE Healthcare). The precleared lysate was incubated with HLA-DR specific antibody L243 (43 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... All purification steps were carried out at 4°C using the ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE healthcare). Protein purity was assessed by SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... These solutions were prepared either by dialysis against the buffer at 4°C or by passage through PD-10 desalting columns (GE Healthcare). Protein concentrations were measured spectrophotometrically using known values for the extinction coefficient ...
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bioRxiv - Immunology 2021Quote: ... 0.5-1 mL plasma or serum was heat inactivated at 56°C for 45 minutes and incubated overnight with Protein G Sepharose 4 Fast Flow beads (GE Healthcare) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were digested at 4°C with specific proteases and further purified by ion-exchange chromatography using a HiTrap SP HP or monoQ (GE Healthcare) columns and a gradient running from 0% buffer A (40 mM TRIS ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-SV40-NLS were cloned by in-frame insertion of the corresponding NFAT5 fragments and SV40-NLS into the C-terminus of GST of pGEX-4T-1 (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...