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Citations for GE Life Sciences :
1401 - 1450 of 1791 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Zoology 2019Quote: ... The gDNA concentration was estimated by Qubit and about 20 ng of gDNA amplified with primers specifically designed to include the intron region (Hsp70 genomic F/R, Table 1) using PuReTaq Ready-To-Go™ PCR Beads (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Cell Biology 2019Quote: ... and imaged using the ChemiDoc-It Imaging System and VisionWorksLS software (UVP, Upland, CA, USA) or Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL). Bands were quantified using densitometry analysis in ImageJ (Abramoff et al. ...
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bioRxiv - Microbiology 2023Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare, Chicago, IL, United States). The signal was visualized in a Chemidoc MP imaging system (Bio Rad)s).
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in the medium for Leydig cells (low glucose DMEM (PanEco) with 10% fetal bovine serum (FBS, HyClone, GE Healthcare Life Sciences, Chicago, IL), 1% penicillin/streptomycin (PanEco) ...
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bioRxiv - Biochemistry 2023Quote: ... USA, 1:10000), luminescence was revealed with Amersham ECL™ Prime reagents (Cytiva, UK) and acquired with ImageQuant™ LAS 4000 (GE Healthcare, Il, USA).
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bioRxiv - Immunology 2021Quote: ... and incubated with 1:1000 diluted Amersham ECL anti-rat IgG horseradish peroxidase-linked species-specific whole antibody (NA935, GE Healthcare UK Limited, UK) or Amersham ECL anti-mouse IgG horseradish peroxidase-linked species-specific whole antibody (NA931 ...
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bioRxiv - Microbiology 2021Quote: Subsamples for chlorophyll a were collected by filtering 125 mL of seawater onto 25-mm diameter GF/F glass microfiber filters (Whatman, GE Healthcare Life Sciences, Chicago, IL, USA), and stored in aluminum foil at −80°C ...
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bioRxiv - Immunology 2024Quote: ... were sourced from a healthy donor with no α-gal allergies (α-gal sIgE <0.10) and isolated through a Ficoll–Paque (GE Healthcare in Chicago, IL, USA) gradient ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and purity of these samples were evaluated on a 1% agarose gel and on a NanoVue spectrophotometer (GE Healthcare Life Sciences, Chicago IL, US). We used an Illumina paired-end TruSeq RNA library preparation kit for 150 sequencing cycles for a mid-output flowcell ...
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bioRxiv - Biophysics 2021Quote: ... to 100% buffer B (6 M urea, 20 mM Tris pH 9.0, 1 M NaCl) of an AKTA FPLC system (GE Healthcare). Fractions containing full-length H1 were pooled and purified on a semi-preparative C18 HPLC column on a gradient of 0—70% buffer B and freeze-dried until use ...
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bioRxiv - Biochemistry 2020Quote: ... SEC-MALS analysis was carried out by injecting 100 μL (A280nm = 1.0) of either sample onto a Superose 6 increase 10/300 GL (GE Healthcare) equilibrated in TBS containing 0.02% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Cell supernatant were collected after centrifuge at 40,000 rpm for 30 min and loaded onto superpose 6 increase (GE healthcare) for FSEC analysis.
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... except with 0.025% (w/v) GDN as the detergent and gel filtration with a Superose 6 Increase 10/300 column (GE Healthcare) following affinity purification.
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bioRxiv - Molecular Biology 2019Quote: ... concentrated with Amicon Millipore 15 ml 100,000 MWCO centrifugal concentrator and applied to Superose 6 increase 10/300 GL column (GE Healthcare) equilibrated in storage buffer (400M KCl ...
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bioRxiv - Microbiology 2019Quote: ... Intact complexes were separated from aggregate by size-exclusion chromatography in TBS containing 0.01% (w/v) LMNG (S200 10/300 increase or Superose 6 increase, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Labeled protein was separated from unreacted fluorophore and the Sfp synthase by size-exclusion chromatography on a superose 6 3.2/200 (GE Healthcare) preequilibrated in 50 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting filtrates were analyzed by size-exclusion column chromatography using Superose 6 Increase 10/150 GL (GE healthcare). Peak values at the retention time for the complex were plotted as a function of incubation temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The target protein was cleaved off the column with TEV protease and subjected to size exclusion chromatography on a Superose 6 Increase 3.2/300 column connected to the Ettan system (GE Healthcare) operated at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... These two complexes were then mixed together with 1.5x molar excess of Nup84-Nup133 and run over a Sepharose 6 10/300 column (GE Healthcare). Fractions containing all Y complex components were pooled and concentrated.
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bioRxiv - Biochemistry 2020Quote: ... Inc) and subsequently subjected to further purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) with the buffer of 75mM Tris (pH7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was further purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... The concentrated CCT single subunit protein was passed over a Superose 6 10/300 GL size exclusion column (GE Healthcare). The individual subunit homo-oligomeric rings or micro-complexes exist around 11–14 ml fraction from the size exclusion column.
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was incubated at 4°C for 1 h with Ni-charged IMAC Sepharose 6 Fast Flow (GE Healthcare). The resin was then washed three times with His-A buffer (lysis buffer + 20 mM imidazole) ...
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bioRxiv - Biophysics 2021Quote: ... OGNG-solubilised HHAT at 1 mg ml−1 was loaded onto a Superose 6 10/300 GL column (GE Healthcare), equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Reaction products were run on a 6% non-denaturing polyacrylamide gel and quantified using a Typhoon 7000 phosphorimager (GE Healthcare) and ImageJ software (76).
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bioRxiv - Microbiology 2020Quote: ... The concentrated HDL was purified based on the difference in their molecular size by size exclusion chromatography using a Superose 6 column (1.5×60cm) (GE Healthcare) Fast Protein Liquid chromatography [V0= 34ml ...
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bioRxiv - Biophysics 2021Quote: ... To remove aggregated protein the protein solution was filtered through a 0.2 µm filter and loaded on a Sepharose 6 10/300 (GE Healthcare) column equilibrated in solubilization buffer for size-exclusion chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... unreacted labelling dye was removed via size exclusion chromatography (SEC) on a Superose 6 Increase column (GE Healthcare Life Sciences). All labeled proteins were flash-frozen in liquid nitrogen in the presence of 35% glycerol and stored at −80 °C until usage.
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Cell Biology 2020Quote: ... Between 3.6 and 3.8 mg of total extract was separated on a Superose 6 10/300 GL column (GE Healthcare, 17517201) operated on a NGC Quest System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... annealed and then the double stranded damaged or undamged product was purified by size exclusion chromatography (Superose 6, GE Healthcare) in buffer 300 (20 mM HEPES pH 7.6 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Immunology 2022Quote: ... Additional purification was then carried out by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) with 2 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by SDS-PAGE on a 6% gel and transferred to a nitrocellulose membrane (0.45 µm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... using Superdex Peptide PC 3.2/300 (for the 5-subunit hGID complex) or Superdex 30 Increase 3.2/300 (for the 6-subunit hGID complex) columns (both GE Healthcare). Three high-mass fractions enriched in cross-linked peptide pairs were collected for MS analysis.
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bioRxiv - Biochemistry 2021Quote: Hexamerization of both wild-type and L396A Lon was tested by gel filtration chromatography using a Superose 6 Increase 3.2/300 column (GE Healthcare). The column was pre-equilibrated in running buffer (25 mM Tris–HCl ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Cell Biology 2019Quote: A375 nuclear extracts and FLAG-USP7-containing protein complexes were applied to a Superpose 6 size exclusion column (GE Healthcare) that had been equilibrated with dithiothreitol-containing buffer and calibrated with protein standards (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was incubated 2 h on ice then applied onto a Superose 6 Increase 3.2/300 gel filtration column (GE Healthcare) equilibrated in 20 mM Tris-acetate (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... Z-stacks of 6 µm (30 sections, 0.2 µm thickness each) were acquired using SoftWoRx Suite 2.0 (Applied Precision, GE). High resolution images were captured on an Elyra PS.1 super resolution microscope (Zeiss ...
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bioRxiv - Biochemistry 2019Quote: ... Base subcomplex was further purified by size-exclusion chromatography using a Superose™ 6 increase 10/300 column (GE Healthcare) pre-equilibrated with base GF buffer (60 mM HEPES pH 7.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis tagged protein was purified by IMAC affinity purification using 0.5 ml of packed Sepharose 6 Fast Flow Ni-NTA Resin (GE Healthcare) pre-washed with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate is then incubated overnight with 400ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) at 4°C with slow rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified U1 snRNP from the heparin column was further purified on the Superose 6 Increase 3.2/300 column (GE Healthcare) in SEC150 buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... A 50 µg sample of HELB was loaded at 0.5 mL/min onto a Superose 6 10/300 size-exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...