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Citations for GE Life Sciences :
1551 - 1600 of 1848 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... the FLAG-tagged Ubr1 was eluted with 1mg/mL FLAG peptide and further purified by a Superose 6 size-exclusion column (GE Healthcare) equilibrated in a buffer captaining 50 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The fractions corresponding to the elution peak were analysed by SDS-PAGE and those containing the TFIIIC complex were collected and loaded into a XK 16/70 Superose 6 pg column (GE Healthcare) equilibrated with 20 mM HEPES pH 8 ...
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bioRxiv - Biophysics 2021Quote: ... Mixtures were incubated at room temperature for 20 minutes and resolved over a Superose 6 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with assembly buffer ...
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bioRxiv - Biophysics 2021Quote: ... and RBM3 (FliF231-438) were applied to a Superdex 200 10/300 column and to a Superose 6 10/300 column (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Biophysics 2020Quote: ... Kif14 constructs were finally separated from the MBP tag by size-exclusion chromatography on a superpose 6 10/300 (GE Healthcare) column equilibrated with BRB80 buffer (80 mM Pipes (pH 6.8) ...
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bioRxiv - Microbiology 2020Quote: Full-length PbCSP was co-complexed with a molar excess of 3D11 Fab and loaded on a Superose 6 Increase 10/300 GL (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Cell Biology 2020Quote: ... Gel filtration chromatography was performed using an AKTA FPLC System with a Superose 6 Increase 10/300 GL column (GE Healthcare) in 50 mM NaPO4 pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 10 mM maltose solution in the lysis buffer and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare) equilibrated with 30 mM HEPES at pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) before being subjected to additional purification by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... or Protein A resin (Pierce) before being subjected to additional purification by size-exclusion chromatography using either a Superose 6 10/300 column (GE Healthcare) or a Superdex 200 10/300 Increase column (GE Healthcare ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... The protein of interest was separated by affinity chromatography using resin attached with Ni-NTA and being subjected to additional purification by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in 20 mM Tris pH 8.0 and 200 mM NaCl.
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bioRxiv - Biochemistry 2022Quote: ... centrifuged at 13,300 rpm for 15 mins at 4°C and then loaded onto a Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with CENP-E gel filtration buffer (50 mM HEPES pH 7 ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 and 2E10.E9 Fab-PvCSPvk247 complexes were purified via Superose 6 Increase 10/300 GL chromatography (GE Healthcare) and concentrated to 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: Full-length recombinant PvCSPvk210 or PvCSPvk247 was complexed with a molar excess of 2F2 or 2E10.E9 Fab and loaded on a Superose 6 Increase 10/300 GL column (GE Healthcare) using an Agilent Technologies 1260 Infinity II HPLC coupled in-line with the following calibrated detectors ...
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bioRxiv - Molecular Biology 2021Quote: 2F2 Fab-PvCSPvk210 or 2E10.E9 Fab-PvCSPvk247 complexes were purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) and diluted to 50 µg/mL ...
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bioRxiv - Biophysics 2022Quote: The low-salt and high-salt fractions were further purified by using gel-filtration column Superose 6 Increase 10/300 GL (GE Healthcare). The low-salt fraction was injected into the column in SEC150 buffer (10 mM Tris 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... Images were acquired with 200 ms exposure time every 4 minutes for at least 6 h at 37°C using Softworx (Applied Precision) software ...
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bioRxiv - Biochemistry 2019Quote: ... This protein was purified by the same method as in (24) but with only a single IMAC purification step followed by preparative size exclusion chromatography on Superose-6 Increase (GE Healthcare) in a 16/60 column ...
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bioRxiv - Molecular Biology 2019Quote: ... and trimeric complexes were affinity purified via 6xHis-tagged RING1B on Ni2+-charged IMAC Sepharose 6 Fast Flow resin (GE Healthcare). 10 mM imidazole was added to lysates during binding ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2019Quote: ... CDK1/CycA was concentrated and the holocomplex separated from free CDK1 by size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare). Peak fractions were analyzed by SDS-PAGE analysis and fractions where CDK1 and CycA co-eluted were pooled ...
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bioRxiv - Biochemistry 2019Quote: ... The pooled protein was further purified by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare Life Sciences). MPEG-1 fractions were monitored using SDS-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein was then concentrated and subjected to size-exclusion chromatography on a Superose 6 5/150 column (GE Healthcare, Germany) in plunging buffer (20 mM TRIS-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated with a 50 kDa MWCO concentrator and subjected to a second SEC on a Superose 6 (10/300 GL, GE Healthcare) with a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 250 kD (size of vRNA-bound FluPolH5N1 monomer) were collected and then transferred onto a Superose 6 Increase column (GE Healthcare) in gel-filtration buffer containing 20 mM HEPES (pH 7.8) ...
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bioRxiv - Genetics 2021Quote: ... Products of were separated using 6% denaturing sequencing gel for 1 hr at 2000 V and detected at fluorescence filter in the Typhoon FLA (GE Healthcare). Rate of cleavage (nucleotide/minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein/phospholipid complexes were separated from the remaining vesicles and soluble protein by SEC using a Superose 6 column (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: The HTT-HAP40 complex was further purified by size exclusion chromatography (SEC) using a Superose 6 10/300 increase column (GE Healthcare) in running buffer 25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: 150 μl sample was analyzed by Superose 6 Inc 10/300 column (Citiva, NY) equipped in ÄKTA purifier (GE healthcare, NY). The elution was conducted at 0.5 ml/min using the same buffer as used for sample at 4 °C
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bioRxiv - Biochemistry 2020Quote: ... The sample was further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein eluent was concentrated and further purified by size-exclusion chromatography (SEC) using a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with W1 buffer ...
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bioRxiv - Biophysics 2021Quote: ... the protein was finally eluted by 230 mM imidazole and further purified through size exclusion chromatography (SEC, Superose 6, 10/300, GE Healthcare) with Lysis Buffer containing 0.001% LMNG ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
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bioRxiv - Microbiology 2021Quote: ... the clarified supernatant was purified on strep-tactin resin (IBA) followed by size-exclusion chromatography on a Superose 6 10/300 column (GE Healthcare) equilibrated with 10 mM Tris-HCl pH 8.0 and 200 mM NaCl as the running buffer (SEC buffer) ...
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bioRxiv - Microbiology 2021Quote: ... was incubated with 20-fold excess of CV3-25 Fab overnight at 4°C before purification on a Superose 6 300/10 GL column (GE Healthcare). The complex peak was harvested ...
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bioRxiv - Cell Biology 2021Quote: ... Some proteins were further purified by size-exclusion chromatography on a Superose 6 10/300 or Superdex 200 Increase column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times with 20x bead volume of TBS using a magnetic tube rack (MagRack 6, GE Life Sciences).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superpose 6 In-crease 10/300 GL or Superdex 200 Increase 10/300 GL; GE Healthcare) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... SDS-gels were stained by “Blue-Silver” Coomassie Staining (Candiano et al., 2004) and washed with water for 6 hours or overnight before imaging with LAS4000 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... ChIP for Rat1-TAP was performed by incubating 500 μl of the solubilized chromatin sample with 20 μl of IgG Sepharose 6 Fast Flow (GE healthcare) for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated in a vivaspin 100,000 MWCO and loaded onto a Superose 6 5/150 size exclusion chromatography column (GE Healthcare Lifescience) to remove thrombin and exchange the buffer to either 20 mm ...
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bioRxiv - Biochemistry 2020Quote: ... The full length hTop2α was first purified by a tandem affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE healthcare) bound to Ni2+ ions followed by a StrepTrap HP column (GE healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... the eluted proteins were concentrated to ∼200 µL and purified by size-exclusion chromatography on a Superose 6 10/300 GL column (GE Healthcare) in 10 mM Hepes pH 7.5 ...