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Citations for GE Life Sciences :
1351 - 1400 of 1848 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Concentrated media was then subjected to size exclusion chromatography using Sepharose 4 fast flow column connected to the ÄKTA prime system (both GE Healthcare, Chicago, IL, USA) and eluted in PBS with a flow rate of 0.5 ml/min ...
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bioRxiv - Biochemistry 2023Quote: ... The flow-through was collected and purified by ion-exchange chromatography on a HiTrap Q HP column (GE Healthcare Life Sciences, Chicago, IL, USA), using Buffer I1 (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... and processed within 2 hours by dilution at 1:1 (v/v) ratio in phosphate-buffered saline solution (PBS) with layering on top of 1 volume of Ficoll-Paque Premium (GE Healthcare, Chicago, IL, USA) and separation by density centrifugation at 500× g for 30 minutes (20°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the flow-through sample containing the ASK1 TBD protein was purified by size-exclusion chromatography on a HiLoad 26/600 Superdex 75 pg column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM HEPES (pH 7.0) ...
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bioRxiv - Biochemistry 2024Quote: ... thawed ASK1 TBD-CRR-KD was subjected to size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... ASK1 TBD was expressed at 25 °C for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... or 25 °C (ASK1 TBD-CRR and ASK1 CRR) for 18 h and purified from Escherichia coli BL21 (DE3) cells using Chelating Sepharose Fast Flow (GE Healthcare, Chicago, IL, USA) according to the standard protocol ...
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bioRxiv - Genetics 2024Quote: ... Meiotic cells were scanned under the DeltaVision microscope (OMXTM V4; GEhealthcare, Chicago, IL, USA) and the super-resolution images were processed with SoftWoRx (Applied Precision, Issaquah, WA, USA).
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bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... and then further purified by gel filtration chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare) in 1% (w/v ...
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bioRxiv - Biophysics 2019Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Microbiology 2020Quote: ... The IMAC elution was then purified by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) in a buffer composed of 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were subsequently centrifuged and applied onto a Superose 6 3.2/300 Increase size exlusion column (GE Healthcare), equilibrated in complex buffer (100 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and elution was collected and concentrated for subsequent gel filtration chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in SEC Buffer (50 mM HEPES-NaOH ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified on an ion exchange column MonoS and gel filtration column Superose 6 (GE Healthcare). Purified protein was concentrated to ∼20 mg/ml in BRB20 buffer (20mM PIPES ...
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bioRxiv - Microbiology 2019Quote: ... The eluted fraction was separated by size exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) and a buffer containing 50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 0.5 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2-3 hours on a roller ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Biophysics 2019Quote: ... One was further purified via size-exclusion chromatography (SEC) with a Superose 6 increase 10/300GL column (GE Healthcare) pre-equilibrated with SEC buffer (0.035% UDM ...
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bioRxiv - Genetics 2020Quote: Complex formation between POLγA and POLγB was tested by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare) connected to an ÄKTA Purifier (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was applied to size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE healthcare) in gel filtration buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was further purified by size exclusion chromatography using a Superose 6 column (10/300 GL, GE Healthcare) equilibrated with 200 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The concentrate was subjected to size-exclusion chromatography (SEC) using a Superdex 6 Increase 10/300 column (GE Healthcare) equilibrated with buffer consisting of 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using Ni-NTA affinity resin (Quiagen) and followed with SEC (Superose 6 Increase, GE Life Sciences), as previously described10 ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 0.5 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2-3 hours on a roller ...
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bioRxiv - Cell Biology 2021Quote: ... Cy3-Tf used for the measurements at 6 kHz (0.167-ms resolution) was generated by incubating 6.5 µM monofunctional sulfo-Cy3 (GE Healthcare) with 6.3 µM human holo Tf (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were separated from unreacted fluorophores by passing over a Superpose 6 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... This amphipol-reconstituted sample was then purified by size exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) at 4 °C in buffer consisting of 150 mM NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... Fractions containing complex I were concentrated using an Amicon Ultra 4 column with 100,000 molecular weight cutoff and loaded onto a Superose 6 Increase 3.2/30 size exclusion column (GE Healthcare). Complex I was eluted in buffer B (30 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mM PMSF and 1x protease-inhibitor cocktail) and loaded onto a Superose™ 6 Increase column (GE Healthcare). Twenty-four fractions of 0.5 mL were collected and precipitated as described (Esteve-Bruna et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The cleaved protein was further purified through SEC in a Superose 6 increase 10/300 GL column (GE Healthcare), in buffer containing 50 mM phosphate pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... 50 mM KAc,1 mM DTT) Further purification was performed using size exclusion chromatography (Superose-6 increase, GE Healthcare) using Shimadzu High Performance Liquid Chromatograph ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 2 hr on a roller ...
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bioRxiv - Biochemistry 2022Quote: ... Then the interest protein was purified by gel-filtration chromatography using a Superose 6 gel filtration column (GE Healthcare) pre-equilibrated with HBS buffer (10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8.5) ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing complex I were concentrated using Amicon Ultra 4 columns with 100,000 molecular weight cutoff and loaded onto a Superose 6 Increase 3.2/300 size-exclusion column (GE Healthcare) connected to an Äkta Ettan system ...
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bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) and incubated for 2-3 h with rotation along the long axis of the tube ...
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bioRxiv - Biochemistry 2022Quote: ... Non-crosslinked and gluteraldehyde crosslinked CCANΔT−CENP-ANuc complexes were injected onto an Superose 6 3.2/300 gel filtration column (GE Healthcare) pre-equilibrated in 20 mM HEPES (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... the complex was centrifuged at 17,000 xg for 10 minutes and then loaded onto a Superose 6 (GE Healthcare) column equilibrated in wash buffer containing 25 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was then concentrated and subjected to size exclusion chromatography on a Superose 6 column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... 3D8 scFv was purified from the supernatant using an IgG Sepharose 6 fast flow (GE Healthcare, Malborough, MA, USA) affinity column ...
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bioRxiv - Microbiology 2021Quote: Size-exclusion chromatography was performed using a Superose 6 10/300 GL analytical column (GE Healthcare, Piscataway, NJ, USA) connected to an ÄKTAxpress chromatography system (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: The eluted proteins were concentrated and subjected to size-exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare) in the PBS buffer ...
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bioRxiv - Immunology 2022Quote: ... The eluted material was further purified by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) running in 20 mM HEPES pH 8.0 and 150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... dialyzed against 20 mM MOPS-NaOH pH 6.7 and loaded onto a 6 ml Resource S column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were first purified with Ni Agarose 6 FF (AOGMA) with the AKTA system (GE Healthcare Life Sciences) and further purified with anti-Flag affinity beads ...