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Citations for GE Life Sciences :
1351 - 1400 of 2467 citations for Human Mitochondrial Genome Maintenance Exonuclease 1 MGME1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 6His-Cdc11-SNAP was biotinylated with BG-Biotin and subsequently immobilized on a CAP chip (Biotin Capture Kit; GE Healthcare, Freiburg, Germany). Preparation of the chip ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from brain tissues or culture cells were extracted using the illustra RNAspin Mini RNA Isolation Kit (GE Healthcare Life Sciences) for reverse transcription with the High-Capacity cDNA Reverse Transcription Kits (ABI Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... according to manufacturer’s instructions and circular DNA was enriched by rolling circle amplification (RCA) with TempliPhi™ amplification kit (GE Healthcare, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were further washed three times for five minutes in PBS-T before being visualised using ECL kit (GE Healthcare Cat. #RPN2232) and ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of protein samples were subjected to immunoblotting for target proteins and immunoreactive bands were visualized using the Amersham ECL western blotting detection kit according to the manufacturer’s protocol (GE Healthcare, Piscataway, NJ). Antibodies for GAPDH or Cyclophilin B were used as loading controls.
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bioRxiv - Molecular Biology 2019Quote: ... random hexamer primers were used following the manufacturer’s instructions for the GenomiPhi V2 DNA Amplification Kit (catalogue # 45-001-221, GE Healthcare Life Sciences).
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bioRxiv - Genetics 2020Quote: We performed WGA on each F2 gDNA cercarial sample using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences, USA) according to Le Clec’h et al ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were developed using the ECL Prime kit (Cytiva, Marlborough, MA) and imaged using Amersham Imager 600 (GE Healthcare, Amersham, Buckinghamshire, UK). Fractionation was repeated with 3 independent sets of cultures and one set of immunoblots is presented as a representative.
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bioRxiv - Microbiology 2020Quote: ... extracted DNA was subjected to multiple displacement amplification (MDA) using the illustra GenomiPhi V2 DNA amplification kit (GE Healthcare, Piscataway, NJ, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA from 30–50 light-treated or dark control embryos was extracted using the illustra™ RNAspin Mini kit (GE Healthcare). 1 μg total RNA was used for reverse transcription with qScript XLT cDNA SuperMix (Quantabio) ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Genomics 2021Quote: Single-cell WGA was done on 10~17 μm or 17~25 μm C1 DNA-Seq IFC (Fluidigm, 100-5763 or 100-5764) via multiple displacement amplification method using illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, 25660031). The WGA products from single cells were mixed according to their tissue origin ...
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bioRxiv - Molecular Biology 2022Quote: ... The probes (PCR amplified from gDNA or binary plasmid using the oligos in Supplemental Table S3) were internally labelled using [α-P32] dCTP (BRIT India) using Rediprime labelling kit (GE healthcare) and hybridisation and washes were performed as described ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed thrice with PBST and the bands corresponding to protein of interest were detected by chemiluminescence using the Amersham ECL Prime Western Blotting Detection Kit (GE Healthcare; RPN2232) in a Fusion FX7 Spectra Multispectral Imaging system (Witec AG ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes were further washed 3x with TBST and once with TBS before visualization of the resulting bands with ECL Advanced Western Blotting Detection Kit (GE Healthcare, #RPN2135) in an Amersham Imager 600 (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... All genes used in the curves were purified with the IllustraTM GFXTM PCR DNA and Gel Band Purification Kit (GE Healthcare, UK) for better efficiency of the standard curves ...
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bioRxiv - Microbiology 2023Quote: ... Anti-rabbit immunoglobulin AP-conjugated secondary antibody was used and reaction results were obtained using CDP Start Detection Reagent kits (GE Healthcare, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein bands were visualized using an ECL kit (Ez WestLumi plus, ATTO, Tokyo, Japan) via chemiluminescence (ImageQuant LAS 4000 mini, GE Healthcare, USA) and quantified using NIH ImageJ software (National Institutes of Health ...
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bioRxiv - Cell Biology 2020Quote: ... or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000, GE Healthcare, Buckinghamshire, UK), and SuperSigna West Femto Maximum Sensitivity Substrate (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: The prepared cell supernatants and sera were first filtered through 1-μm filters (GE Healthcare Whatman Puradisc GMF ...
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bioRxiv - Immunology 2021Quote: ... the mAb was purified using a 1 mL HiTrap Protein A HP column (GE Healthcare). The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...
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bioRxiv - Systems Biology 2022Quote: ... The filtered lysate was loaded onto a 1 ml HisTrap FF (GE Healthcare, Freiburg, Germany) and unbound protein was removed with 20 column volumes of 15% buffer B (50 mM HEPES/KOH pH 7.8 ...
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bioRxiv - Cell Biology 2019Quote: ... The following horseradish peroxidase-conjugated secondary antibodies were used: anti-rabbit (GE Healthcare; 1:3000), anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Biophysics 2019Quote: ... used at 1:5000 dilution and detection with peroxidase-labelled secondary antibodies (GE Healthcare, UK) and ECL Plus (enhanced chemiluminescence ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies were added as follows: biotinylated anti-mouse 1:200 (GE Healthcare RPN 1001) followed by Tyramide Super Boost with Alexa Fluor 488 1:500 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... After 1 hour the excess dye was removed via PD-10 desalting column (GE Healthcare) equilibrated with the storage buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... 1:3000 dilution) and then with anti-mouse or anti-rabbit secondary antibodies (GE Healthcare 1:5,000 dilution in 5% dry milk and 1X TBST) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM DTT) and purified by cation exchange chromatography (MonoS 5/50 GL, GE Healthcare) on an AKTA pure chromatography system (buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM DTT) and purified by cation exchange chromatography (MonoS 5/50 GL, GE Healthcare) on an AKTA pure chromatography system (GE Healthcare) ...
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bioRxiv - Genetics 2020Quote: ... Anti-rabbit IgG or anti-mouse IgG conjugated with horseradish peroxidase (GE Healthcare, 1:3,000) and ECL (Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-Rabbit IgG Horseradish Peroxidase (HRP)-linked whole antibody (GE Healthcare, #NA934, 1:10000).
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bioRxiv - Biochemistry 2020Quote: ... the 5’ half of the tRNA (nucleotides 1-39) was chemically synthesized (GE Healthcare Dharmacon) and enzymatically ligated to the chemically synthesized 3’ half following established protocols (52 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM benzamidine) and loaded on a 5 ml HiTrap IMAC HP column (GE Healthcare). After a wash step for 3 column volumes in 30% IMAC buffer B (same as IMAC A but 500 mM imidazole) ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibodies used were ECL-anti-rabbit HRP IgG (GE Healthcare, NA9340V, 1:10000), ECL-anti-mouse HRP IgG (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... and HRP-linked anti-mouse (GE Healthcare, #NA931-1ML, at a dilution of 1:5000) as the secondary antibody ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were developed with goat anti-rabbit HRP antibody (1:10,000; GE Healthcare #NA934V) and ECL reagent (GE Healthcare #RPN2106).
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bioRxiv - Microbiology 2020Quote: ... mAb followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2,000, NXA931, GE Healthcare). Periplasmic fractions ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated with streptavidin conjugated to horse radish peroxidase (1:5000) (GE Healthcare, RPN1231VS) in 1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and protein was eluted by ca 40 units PreScission protease (GE Healthcare) for 4 hours at 4°C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then incubated with [3H]acetic acid (1 μCi/well; Amersham, GE Healthcare) in serum free DMEM F-12 for 4 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies at 1: 5000 dilution (GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and then for another 1 h following the addition of protein-G Sepharose (GE Healthcare). Protein bound to antibody beads was washed five times with lysis buffer at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was then SEC purified over a Superdex 200 1/150 column (GE Healthcare). The peak corresponding to complex was pooled and concentrated to 1.0 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... The ITC protein sample p52 (1-398) went through desalting column (HiTrap desalting, GE Healthcare) to freshly made ITC buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h at room temperature and visualized using Enhanced Chemo-Luminescence (ECL) (GE Healthcare) or Typhoon Biomolecular Imager (GE Healthcare) ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated overnight in 8M rehydration solution containing 1% IPG pharmalyte pH3-10 (GE Healthcare). After focusing ...
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bioRxiv - Biophysics 2022Quote: ... to ~ 1 mL and further purified on a Superdex 200 10/300 Increase (GE Healthcare) column equilibrated with buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged ZO-1-PDZ2 were purified using a HiTrap Chelating HP (GE Healthcare), where a renaturation gradient by exchange of denaturation buffer to a gel filtration buffer (Tris 50 mM (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was then loaded on a 1 mL HisTrap FF Crude column (GE Healthcare) with flow rate of 1 mL/min using the AKTA FPLC ...