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Citations for GE Life Sciences :
1551 - 1600 of 2467 citations for Human Mitochondrial Genome Maintenance Exonuclease 1 MGME1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Staining was detected using an HRP-conjugated anti-rabbit secondary antibody and Chemi-Lumi One L assay kit (Nacalai) on a ImageQuant LAS 4000 imager (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Microbiology 2020Quote: ... the cDNA obtained in the section “Realtime and Endpoint RT-PCR detection of deltaviruses from animal specimens” was amplified using illustra GenomiPhi V2 Kit (GE healthcare; Chicago, IL, USA). The amplified DNA was then purified with innuPREP PCRpure Kit (Analytik Jena ...
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bioRxiv - Evolutionary Biology 2019Quote: ... on each FTA punch or on 2 µL or 4 µL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life TM Sciences). Amplified DNA was purified with the SigmaSpin Sequencing reaction Clean-up (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... Membranes were again washed with 4 x 15 min TBS-T washes and a final 15 min TBS wash prior to band development with an ECL Prime Western Blot Detection Kit (GE Healthcare, Chicago, IL, USA) and imaged using a Chemidoc Imaging system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5) was immobilized on the surface of a CM5 sensor chip using a Amine Coupling Kit (GE Healthcare Bio-Sciences AB, Sweden). VHHs were serial 4-fold diluted from 417 nM to 0 nM and flowed over the captured RBD surface at 15 μl/min for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The samples were transferred to a positively charged membrane using southern blot protocol from the Whatman Nytran SuPerCharge TurboBlotter kit (Whatman GE Healthcare, Little Chalfont, UK). Air dry the membrane ...
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bioRxiv - Microbiology 2022Quote: ... Multiple displacement amplification of genomic DNA was performed using the illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit (GE Healthcare, Piscataway, NJ, USA). Paired-end library was constructed using NEXTFLEX Rapid DNA-Seq (Bioo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... PCR samples were resolved on agarose gel and amplified fragments extracted from gel using GFX PCR DNA and Gel Band purification kit (GE Healthcare, 28-9034-70). The purified DNA or 1µg of plasmid DNA were digested with XmaI and XbaI for 1 h at 37°C in 1x CutSmart buffer and nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... The remainder of the extraction was performed with a modified version of the Illustra RNAspin mini RNA isolation kit (GE Healthcare, #25-0500-71) protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The PuACO1 or PuERF2 CDSs were inserted into the pGEX4T-1 vector (GE Healthcare, http://www3.gehealthcare.com) downstream from GST ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNA encoding mouse TRIM41 (residue 35-85 aa, NP_663352.2) was inserted into pGEX6p-1 (GE healthcare), and the expression vector was transformed into E ...
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bioRxiv - Cell Biology 2020Quote: ... peroxidase-conjugated sheep anti-mouse IgG antibody diluted in 1:4000 (NA931, GE Healthcare, Little Chalfont, UK); and peroxidase-conjugated donkey anti-rabbit IgG antibody diluted in 1:2000–1:4000 (NA934 ...
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bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with a 1:5000 dilution of anti-rabbit (GE Healthcare, Little Chalfont, UK, NA934V) or anti-mouse secondary antibody (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM β-mercaptoethanol) and further purified by gel filtration over a Superdex-200 column (GE Healthcare). The protein product was concentrated with a centrifugal filter unit (MD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated in horseradish peroxidase-labeled goat anti-rabbit or goat anti-mouse antibodies (GE Healthcare; 1:10,000) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Biophysics 2020Quote: ... the sample supernatant fraction was incubated with 1 ml Glutathione Sepharose 4B (GE Healthcare, 17-0756-01) for 3 h at 4 °C to bind GST-tagged proteins ...
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bioRxiv - Bioengineering 2019Quote: ... Whatman Grade 1 chromatography paper for the paper-based reactions was obtained from GE Healthcare (Mumbai, India). DNA ladder (O’GeneRuler ultra low range 10–300 bp ladder) ...
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bioRxiv - Molecular Biology 2020Quote: ... 12.5% acrylamide:bis-acrylamide 19:1) electrophoresis and heat/vacuum dried for autoradiography (Amersham Hyperfilm ECL, GE Healthcare). Images were analyzed using ImageJ ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM β-mercaptoethanol and loaded onto a 1 ml MonoQ anion exchange chromatography column (GE Healthcare). After washing with 10 column volumes of buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HRP conjugated secondary antibodies were used at 1:10,000 and visualised using ECL (GE Healthcare, Buckinghamshire, UK), high performance chemiluminescence film (Amersham Hyperfilm ECL ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 and 1 mM CaCl2 and applied to a 5 mL HisTrap Excel (GE Healthcare). Afterwards the column was washed with 40 mL of 20 mM TRIS pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... 1% saline-sodium citrate [SSC]) at 50 °C and exposed to a phosphor screen (GE Healthcare, USA) overnight ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK). Ban quantification was done using Image LabTM v ...
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bioRxiv - Biochemistry 2021Quote: Calorimetric titration of Mint3NT with FIH-1 was performed using a MicroCal™ iTC200 system (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal His6-tag was then removed by digestion with thrombin (≈ 1 U/mg; GE Healthcare) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Japan) was subcloned into the BamHI and XhoI sites of pGEX-4T-1 vector (GE Healthcare, Inc.) using standard PCR-based protocols ...
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bioRxiv - Biochemistry 2020Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Eranthie Weerapana) for 1 h at 22 °C and desalted (PD Miditrap G-25 columns, GE Healthcare). Each light lysate was then combined 1:1 with a heavy lysate in 1 mL total volume and incubated with 2% SDS and 2 M urea final concentration in PBS in a final volume of 2 mL for 40 min at 22 °C ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA (pH 8.0)) and further purified on a Source 15Q anion exchange column (GE Healthcare) with the application of 50 mL buffer I (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded at 1 mL/min onto a Ni-NTA column (HisTrap HP, 5 mL; GE Healthcare) that had been previously equilibrated with Ni-NTA Buffer (NB ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µM all samples) separately was loaded (300 µl) in a superdex-200 increase column (GE Healthcare) attached to a Malvern Panalytical Omnisec resolve-reveal system ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated for 1 min with HRP substrate (ECL Prime Western Blotting Detection Reagent (GE Healthcare) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo-Fisher-Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... the membranes were probed with 1:2,500 goat anti-rat HRP-linked secondary antibodies (GE healthcare, NA935) in 5% milk powder/PBS solution for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 1 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare, 17096902) for POT1 or 1 mL amylose beads (New England BioLabs ...
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bioRxiv - Physiology 2020Quote: ... All membranes were incubated with secondary horseradish peroxidase conjugated antibody (1:10000; GE Healthcare, Chicago, Illinois, USA) for 1-2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and sheep anti-mouse IgG conjugated with Horseradish Peroxidase (1:10000 dilution, GE Healthcare UK Limited, UK). Band quantification was done using Image LabTM v ...
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bioRxiv - Cancer Biology 2022Quote: ... WT and mutant Ankrd26 N-Ank modules were generated by cloning into pGEX-6P-1 (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... generated by cloning of corresponding ORFs between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare), expressed PhoPN ...
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bioRxiv - Biochemistry 2019Quote: ... Cleared lysates were supplemented with 1 mM DTT and incubated with GSH-Sepharose 4B matrix (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Samples seeded on #1 thickness coverslips were imaged on a DeltaVision Core light microscopy system (GE Healthcare) using either a 60x/1.35na or 100x/1.4na objective fitted to an Olympus IX-71 microscope stand ...
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bioRxiv - Cell Biology 2019Quote: ... Siz1 (167-508) or Siz2 (130-490) was purified from MBPTrap HP 1 mL column (GE Healthcare) with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... FLAG eluate was loaded onto a 1 mL HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic bump and washed with 5 column volumes of 26S NiA buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
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bioRxiv - Physiology 2019Quote: ... and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...