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Citations for GE Life Sciences :
1301 - 1339 of 1339 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 25 μg of protein was separated on a 12% SDS-PAGE gel and transferred to a Hybond-LFP PVDF membrane (GE Healthcare, Illinois, USA). The membrane was blocked in 5% skimmed milk in 1X Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2024Quote: ... For transphosphorylation assays the kinase was autophosphorylated as described before and excessive ATP was removed using illustra™ MicroSpin™ G-25 Columns (GE healthcare). Afterwards the phosphate receptor protein (10 µM ...
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bioRxiv - Biochemistry 2024Quote: ... each studied sample—the entire EC and entire MB prepared from each hemi-forebrain—was incubated with 40 µL of Protein G Sepharose 4 Fast Flow resin (GE healthcare, Piscataway, NJ) slurry (volume of settled beads:volume of storage buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... The α-syn oligomers and free α-syn from the supernatant were separated using a Superdex 200 Increase 10/300 GL (GE Healthcare, 28990944) equilibrated with PBS ...
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bioRxiv - Physiology 2020Quote: ... U2OS-ERα cells were cultured in phenol-free αMEM medium supplemented with 10% HyCloneTM charcoal/dextran stripped FBS (GE Healthcare Life Sciences, Pittsburgh, PA), 1% antibiotic/antimycotic ...
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bioRxiv - Neuroscience 2022Quote: ... The labelled protein was purified from the excess of free dye by a P10 desalting column with Sephadex G25 matrix (GE Healthcare, Waukesha, WI, USA), divided into aliquots ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Immunology 2020Quote: Approximately 200 μL of normal mouse serum or anti-CD200 serum day 157 (after 4X immunizations) were purified using a protein G SpinTrap (GE Healthcare # 28-4083-47) according to the manufacturer’s instrutions ...
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bioRxiv - Neuroscience 2020Quote: ... in which they were precleared for 1 h using 30 μl (dry volume) of a 1:1 mixture of protein-A and protein-G-agarose beads (GE Healthcare, Chicago, IL, USA). Thereafter ...
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bioRxiv - Biochemistry 2020Quote: ... The clear upper phase of the lysate was desalted by size exclusion chromatography using a PD MiniTrap G-25 prepacked column (GE Healthcare, Chicago, IL, USA) with assay buffer as eluent ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was subjected to serial purification steps using Superdex 200 Increase 10/300 G size-exclusion and Hitrap Q Sepharose Fast Flow anion-exchange columns (GE Healthcare, Pittsburgh, PA, USA). Purified a-syn was applied to High capacity endotoxin removal spin columns (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 μg of total protein from the supernatants was precleared with Protein G Sepharose 4 Fast Flow (GE Healthcare, catalog #GE17-0618-01) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Microbiology 2021Quote: ... Primers were labelled using T4 PNK in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol) and purified using G-50 Microspin columns (GE Healthcare, #27-5330-01). Reverse transcription reactions were stopped by adding 40 μL of cold 0.3 M Na-acetate ...
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bioRxiv - Biochemistry 2021Quote: ... reaction mixture was terminated by heating and limit-digested products were recovered by doing centrifugation (10000 × g) for 10 min and then applied to Toyopearl S40 resin containing column (GE Healthcare-XK 26/100) for purification ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing proteins were loaded onto the HiTrap Desalting 5 x 5 ml (Sephadex G-25 S) (GE Healthcare, Code#: 17-1408-01) with a running condition of 50 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of total RNAs were fractionated by formaldehyde-agarose gel electrophoresis and transferred to Amersham™ Hybond-XL nitrocellulose membrane (GE Healthcare, Cat# RPN203S) by capillarity ...
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bioRxiv - Neuroscience 2023Quote: ... A small volume of the bead-coupled GST-fusion proteins was loaded into a ProbeQuant G-50 Micro Column (GE Healthcare, cat no. 28903408) and washed once in ice cold lysis buffer containing 1 % (v/v ...
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bioRxiv - Biophysics 2024Quote: ... Dyes were incubated with G-actin at a 2-fold molar excess for 2 hours at room temperature before being separated from the labeled protein by applying the labeling reaction to a spin column packed with Sephadex G-50 Fine DNA Grade (GE Healthcare GE17-0573-01) hydrated with A buffer (5 mM Tris-HCL (pH 8) ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... 150 μl of DEPC-treated water was then added and the reaction passed through a home-poured G-25 sephadex (GE Healthcare cat. # 17-0033-01) spin column ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Genomics 2022Quote: ... 0.5ml of antibiotic antimycotic solution (10000 lg/ml streptomycin, 10000 U/ml penicillin G, and 25 lg/ml amphotericin B, HyCloneTM, GE Healthcare Life Sciences, Amersham, UK) and 6ml of foetal calf serum were used to make lymphocyte short-term medium ...
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bioRxiv - Cell Biology 2022Quote: ... A tenth of the lysate was taken as TCE control and the rest was added to washed Protein G-coupled Sepharose beads (Sigma-Aldrich GE Healthcare 17-0618-01) for preclearing (30 min) ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... The LacO-specific probe was end labeled with 32P-γ-ATP for 1 h at 37 °C before cleaning with illustra ProbeQuant G-50 micro column (GE Healthcare; 28-9034-08). The blot was incubated for 2 h at 42 °C in hybridization buffer (ULTRAhyb™ Ultrasensitive Hybridization Buffer [Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Twenty μg samples were loaded into SDS-PAGE gels and transferred onto Amersham Hybond-P 0.45μm PVDF membranes (GE Life Sciences, Pittsburgh, PA; CAT: GE 10600029). Membranes were incubated in Fisher No-Stain Total Protein Stain (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... Concentrated protein was then subjected to single step buffer exchange with Dialysis Buffer (20mM Tris-Cl, pH 8.0, 50mM NaCl) with the help of aPD10 desalting column (GE Healthcare, Sephadex G-25 M matrix) under gravity flow ...
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bioRxiv - Immunology 2022Quote: ... The cell free supernatant derived during harvest of the fermented broth was processed using Blue Sepharose™ 6 Fast Flow column (GE Healthcare, Cat# 17-0948-01) in flow-through mode followed by a hydrophobic interaction chromatography (HIC ...
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bioRxiv - Microbiology 2022Quote: ... Samples (100 µL OXAVL02 or OXAVL06 at 0.5-1 mg/mL) were loaded onto a Superdex® 200 Increase 10/300 G column (GE Healthcare Bio-Sciences AB Uppsala, SW) pre-equilibrated with the “SECMALS-PBS buffer” ...
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bioRxiv - Neuroscience 2021Quote: ... and subjected to single-step buffer exchange into SEC running buffer (see SAXS method) over a PD10 desalting column (GE Healthcare, Sephadex G-25 M matrix) under gravity flow ...
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bioRxiv - Biochemistry 2020Quote: ... The redissolved protein sample was desalted using a NAP10 desalting column pre-packed with Sephadex G-25 resin (NAP™-10 Columns, Sephadex™ G25, GE Healthcare Life Sciences, UK) and eluted in 20 mM sodium phosphate buffer (pH 6.0) ...
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bioRxiv - Pathology 2023Quote: ... homogenates were centrifuged at 500 x g for 5 min at RT and cell pellets were resuspended in a 36% Percoll solution (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) containing 100 UI/mL of heparin (HIBOR 5000 UI ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble chromatin was pre-cleared by incubation with 10 μL of 50% RIPA-equilibrated Protein A + G sepharose bead slurry (GE Healthcare, Cat. No 17-5280-11 and 17-0618-06) for every 100 μL of chromatin for 1 h at 4°C ...