Labshake search
Citations for GE Life Sciences :
951 - 1000 of 1339 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... 50 µg of purified GST-HA-SspH1 was mixed with 2 µg in-house purified GST-tagged PreScission protease and pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare) in PreScission protease buffer (50 mM Tris pH 7.5 ...
-
bioRxiv - Microbiology 2024Quote: ... The 6ξ-His tag was cleaved for 16 h at 4 °C using 1U of thrombin per 100 µg of total protein (GE Healthcare). The cleaved proteins were then passed through a Ni-NTA column to remove the cleaved 6ξ-His tag and any uncleaved protein ...
-
bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
-
bioRxiv - Cell Biology 2024Quote: ... equal volumes (125 μl containing 6250 µg) of two different kind of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was isolated from samples using illustra RNAspin Mini RNA Isolation Kit with RNase-free DNase I (GE Healthcare) according to the manufacture’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... The complex was separated from free D3_Sof protein by size-exclusion chromatography using “Superdex 75 10/300 GL” column (GE Healthcare) at 0.4 ml/min flow rate using running buffer containing 25 mM Tris-HCl (pH 7.6 at 25 ° C) ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed with cold phenol red-free DMEM/10% FBS buffered by 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses ...
-
bioRxiv - Biochemistry 2022Quote: ... and then separating the complex from free GTPase by a HiLoad 26/60 Superdex 200 gel filtration column (GE Healthcare), followed by a RESOURCE™ Q column ...
-
bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed in PBS and incubated overnight in Cy3-conjugated streptavidin (1:1000 dilution; PA43001, GE Healthcare) in “Triton-PBS” (PBS containing 0.3% v/v Triton X-100 [Sigma]) ...
-
bioRxiv - Biochemistry 2022Quote: ... ΔCter and free p85α were further subjected to gel filtration using SuperdexTM 200 10/300 GL Increase from GE healthcare. After gel filtration ...
-
bioRxiv - Developmental Biology 2020Quote: ... Minced mammary tissues were then digested in phenol-red free Dulbecco’s Modified Eagle Medium (DMEM) (Nacalai Tesque) containing 2mM L-glutamine (GE healthcare), 1mg/ml collagenase (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2021Quote: ... Free dye was removed by size exclusion chromatography using a HiPrep 16/60 Sephacryl S 100 HR column (GE Healthcare) equilibrated with PBS buffer ...
-
bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
-
bioRxiv - Neuroscience 2022Quote: ... The protein was separated from free dye using gel filtration on a Superdex 75 pg 10/300 column (GE Healthcare).
-
bioRxiv - Immunology 2024Quote: ... Conjugated RBD-mi3 particles were separated from free RBDs by SEC on a Superose 6 10/300 column (GE Healthcare) equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: Conjugated RBD-mi3 particles were separated from free RBDs by SEC on a Superose 6 10/300 column (GE Healthcare) equilibrated with PBS (20 mM sodium phosphate pH 7.5 ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed with cold phenol red-free DMEM with 10% FBS and 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses and incubated at 37 °C to initiate virus-cell fusion ...
-
bioRxiv - Immunology 2022Quote: ... Conjugated SOSIP-mi3 particles were separated from free SOSIPs by SEC on a Superose 6 10/300 column (GE Healthcare) equilibrated with PBS ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant obtained by centrifugation (19,000 × g, 15 min, 4°C) was applied to a His SpinTrap column (GE Healthcare) previously equilibrated with buffer A ...
-
bioRxiv - Biophysics 2021Quote: ... Cell lysates were centrifuged at 75,000 x g for 25 min and supernatants were loaded on a GSTrap FF 1 mL column (GE Healthcare) using an AKTA Start purification system (GE Healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was recovered by centrifugation (50,000 g, 20 min, 4°C) and applied to a HisTrap FF NiNTA column (GE Healthcare). After washing with buffer A (50 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: Cells lysates were prepared using 1 % triton lysis buffer and precleared with prewashed Protein G Sepharose (PGS) beads (GE Healthcare) for 30 min on a shaker at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... The cell lysate was then centrifuged at 18,800 × g for 10 min and the resulting supernatant was applied to His Spin Trap (GE Healthcare). After centrifugation (100 × g ...
-
bioRxiv - Physiology 2022Quote: ... Supernatants were collected after centrifugation at 13,200 rpm for 15 min and precleaned for 1 h with 50% protein G-Sepharose (GE Healthcare), followed by incubation with an indicated antibody at 4 °C Upon addition of 100 μl of 50% protein G-Sepharose ...
-
bioRxiv - Molecular Biology 2020Quote: ... For each IP 100µL chromatin was pre-cleared with BSA blocked protein-G sepharose beads (GE Healthcare, Chicago, IL, USA) and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449 ...
-
bioRxiv - Physiology 2020Quote: ... Cell pellets were resuspended with Medium 199 X1 and cells subsequently separated using a three layer Percoll density gradient (1.028, 1.05 and 1.088 g/ml) according to manufacturer instructions (Percoll plus, GE Healthcare Life Sciences) and as described previously83 by centrifuging at 600g for 30 minutes with controlled acceleration and braking ...
-
bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 25,000 × g for 30 min and were then bound to Ni-NTA beads (GE Healthcare). After extend washing with buffer containing 50 mM imidazole ...
-
bioRxiv - Biophysics 2020Quote: ... which is complementary to the tracrRNA 5’-end was labeled with ATTO647N-NHS (1:60 DNA:dye molar ratio) and purified using G-25 micro spin column (Illustra, GE Healthcare). The measured labeling efficiency of ATTO647N-oligonucleotide was >80% ...
-
bioRxiv - Biophysics 2021Quote: ... The dye-labeled protein was purified using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50000 rpm for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: ... The suspension of solubilized protein was ultracentrifuged at 100,000 × g for 1h before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with buffer containing 20 mM Tris-Cl ...
-
bioRxiv - Biochemistry 2020Quote: ... for 30 minutes with or without cofactor GMPPNP (30 μM) then applying 0.5 mL to a PD-10 Sephadex G-25 gel-filtration column (GE Healthcare). Eluted fractions (0.5 mL ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 48,284 g for 45 min at 4°C and loaded into a HisTrap FF cartridge (GE Healthcare). Proteins were eluted with 100 mM HEPES ...
-
bioRxiv - Bioengineering 2020Quote: ... and 35 ml of this suspension was carefully added to 15 ml Ficol-Paque PLUS (1.077 g cm-3, GE Healthcare) and centrifuged at RT ...
-
bioRxiv - Biochemistry 2020Quote: ... reactions were ended with 30 mM EDTA and unincorporated nucleotides were removed using Illustra MicroSpin G-50 columns (GE Healthcare). Samples were then separated on 0.6% alkaline agarose gels (30 mM NaOH and 2 mM EDTA ...
-
bioRxiv - Biochemistry 2021Quote: ... The excess Cu(II) was eliminated by passing the protein through a PD MidiTrap G-25 desalting column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 and 150 mM NaCl.
-
bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
-
bioRxiv - Cell Biology 2021Quote: ... The cell lysate was centrifuged at 38,000 ×g and the supernatant was loaded onto a 5 mL HisTrap Ni-NTA column (GE Healthcare). The column was washed with HisTrap Buffer and protein eluted with a linear gradient of imidazole from 25 to 300 mM in HisTrap Buffer over 10 column volumes.
-
bioRxiv - Microbiology 2020Quote: ... The lysate was centrifuged for 30 min at 30.000x g at 4 °C and loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mmol L−1 Hepes at pH 8 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The chromatin was then precleared overnight at 4°C with vertical rotation at 15rpm (a total of 5 reactions per condition in 15ml tubes) with 500µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) beads and then used for immunoprecipitation.
-
bioRxiv - Molecular Biology 2021Quote: ... and immunoprecipitated again in a single reaction with 100µls of a mix of Protein G and A SepharoseTM 4 Fast flow (GE Healthcare) pre-conjugated with histone H3 and histone H2B antibodies in order to specifically enrich for chromatin-associated dCas9 complexes and eliminate from the purification unspecific non-chromatin associated dCas9 complexes ...
-
bioRxiv - Plant Biology 2021Quote: ... The membrane extract was centrifuged for 35 mins at 200,000 x g and the supernatant loaded onto a 10 ml Nickel column (Chelating Sepharose; GE Healthcare) equilibrated in TSB with 0.2% DDM pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
-
bioRxiv - Microbiology 2020Quote: ... and the others were incubated with the indicated antibodies overnight at 4°C.Then mixed with the Protein G sepharose beads (GE Healthcare) for 2 h at 4°C.The immunoprecipitates were washed four to six times with the 293T lyses buffer (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... with a JA30.50 Ti rotor at 20,000 ×g and applied to a HiTrap SP HP column (GE Healthcare; 1 ml) equilibrated in buffer A (20 mM Na-phosphate buffer (pH 7.0) ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were cleared by centrifugation for 1 hour at 38,700 x g and passed over a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 15 column volumes of 20 mM Tris ...
-
bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
-
bioRxiv - Biophysics 2020Quote: ... The lysate was centrifuged at 20,000 x g and the supernatant was loaded onto a StrepTrap HP column (GE Healthcare). Protein was eluted in binding buffer with 2.5 mM D-desthiobiotin ...
-
bioRxiv - Cell Biology 2021Quote: ... Insoluble material was sedimented by centrifugation at 13,500 g for 30 min at 4°C prior to loading on His-trap FF columns (GE Healthcare) pre-equilibrated with lysis buffer ...