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Citations for GE Life Sciences :
1001 - 1050 of 1339 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... concentrated to 0.5 ml and after centrifugation at 21,700 g for 20 min directly applied to a Superose 6 Increase 10/300 GL column (GE Healthcare) in SEC buffer (50 mM TRIS pH 7.8 ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysate was centrifuged at 45,000 x g for 35 minutes and supernatant was loaded onto a HisTrap HP affinity column (GE Healthcare). A linear gradient of buffer B (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Fractions between 8.5 and 10 mL were pooled and concentrated by centrifugation at 350 x g using Vivaspin filters with a 10,000 kDa molecular weight cutoff (GE Healthcare). Concentrated GPC-I53-50NPs were then diluted 1:1 with TBS ...
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bioRxiv - Biophysics 2022Quote: ... The sample was exchanged into the D2O buffer using a NAP-5 Sephadex G-25 column (GE Healthcare, Chicago, IL). For dUMP and TMP (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant after centrifugation at 17,000 g at 4 °C for 1 hr was incubated with Glutathione Sepharose 4B (GE healthcare) at 4 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Antibody supernatants were harvested seven days following transfection and purified using protein G affinity chromatography following the manufacturer’s protocol (GE healthcare). In order to validate the functional relevance of Arg100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris and unlysed cells were pelleted at 48,000 × g and the supernatant was incubated with glutathione beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... supernatant was adjusted to pH=7 and loaded onto a High-Trap Prot G column (GE Healthcare, Chicago, Illinois, USA) using an Äkta system (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... After centrifugation at 60,000 × g the supernatant was used for purification using nickel-affinity chromatography on a HisTrap HP column (GE Healthcare), using an isocratic elution of low to high imidazole buffer (500 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Protein G-coupled sepharose (#17–0618–01) and Protein A-coupled sepharose (#17–5138–01) beads were from GE Healthcare and the protease inhibitor cocktail was from Sigma.
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bioRxiv - Immunology 2022Quote: ... Polyclonal IgG from subject 391370 was isolated from heat-inactivated plasma using Protein G Sepharose 4 Fast Flow (GE Healthcare). Purified IgG was buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was then centrifuged for 1 h at 16,000 g at 4 °C and the supernatant was added to 1 mL of glutathione beads (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... all Fabs were expressed in ExpiCHO cells and purified using a HiTrap Protein G HP column (GE Healthcare, Chicago, IL) followed by size exclusion chromatography as used for the IgG1 but in Tris Buffered Saline (TBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The unreacted DBCO molecules were eliminated by a Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences). To make fluorescently labeled DBCO modified FBS proteins or individual proteins (BSA or APO H) ...
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bioRxiv - Developmental Biology 2020Quote: ... After the buffer was replaced with 20 mM sodium phosphate (pH 5.5) buffer containing 25 mM NaCl using a PD MiniTrap G-25 column (GE Healthcare), the digested sample was applied to a HiTrap Q HP column and every 1.5 ml was collected during the application and washing with the same buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation for 1 hour at room temperature proteins were purified using PD MiniTrap™ G-25 columns (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at 4 °C in an overhead tumbler before retrieving immune complexes with protein G Sepharose (GE Healthcare). Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Ab Spin Trap columns (immobilized protein G) and NHS-activated sepharose® 4 fast flow were purchased from GE Healthcare Life Sciences (Buckinghamshire ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were cleared by centrifugation at 100,000 g at 4°C for 30 min and then mixed with Glutathione Sepharose 4B (GE Healthcare). The Sepharose beads were washed with buffer B [50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled NBD1 was spun at 4 °C and 16,000 g for 30 minutes and passed through a gel filtration column (Superdex 200 Increase 10/300 GL - GE Healthcare) previously equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifuging at 27,000 x g and the supernatant was applied to a HiTrap-Ni column (GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 33,000 g and loaded on pre-equilibrated Ni-Sepharose 6 Fast Flow (HisTrap FF, GE Healthcare). The column was washed with 20-30 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... DTT was removed via two consecutive passes through a PD-10 Sephadex G-25 M buffer exchange column (GE Healthcare) as per the manufacturer’s instructions into labelling buffer (50 mM HEPES pH 7.1 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was centrifuged for 10 min at 16,000 × g and the supernatant was incubated with 100 μl (settled volume) of IgG agarose beads (GE Healthcare) at 4°C for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Immunology 2021Quote: ... IgG and IgM were separated from serum by affinity chromatography using HiTrap Protein G High Performance column (Cytiva, GE Healthcare) and CaptureSelect IgM Affinity Matrix (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was clarified by centrifugation at 13,000 x g for 35 min and was passed over Ni-NTA sepharose (GE Healthcare) column ...
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bioRxiv - Biophysics 2022Quote: ... The solution was subjected to a 5 min spin at 17,000 x g in a refrigerated microcentrifuge before separation on a Superdex 200 10/300 column (GE Healthcare) equilibrated with SEC buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... the sample was clarified 400,000 x g for 30 minutes and applied to a Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column equilibrated in G-buffer containing 0.2 M ammonium acetate ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 40 min and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700 x g for 12 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 1 h and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min at 4°C and incubated for 45 min with amylose beads (GE Healthcare). The loaded protein was cleaved on column by GST-tagged 3C protease at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... and further purified by affinity chromatography on an affinity column containing the above mentioned connexin36 segment protein coupled to CNBr-activated Sepharose (14 mg to 2.5 g Sepharose 4B; GE Healthcare). The specificity of the affinity purified antibodies was tested on tissue extracts and cryostat sections (12 µm thick ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was spun down at 400× g for 5 minutes followed by resuspension in a an isotonic 33.75% (v/v) Percoll (GE Healthcare) diluted in PBS with 2% foetal calf serum and density gradient centrifugation at 700x g for 12 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The sonicated proteins were pre-cleared with a 25μl protein A sepharose and protein G sepharose beads mixture (GE Healthcare) and the appropriate antibodies were added to the cleared extract and incubated overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... followed by centrifugation at 20,000 x g where the clear lysate was subsequently loaded onto a 5 mL Ni-NTA Sepharose column (GE Healthcare) equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 39 000 × g for 40 min and the supernatant loaded onto StrepTrap column (GE Healthcare) in lysis buffer containing 50 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... The soluble fraction was separated by centrifugation at 30,000×g for 30 min and subjected to size exclusion chromatography using a HiLoad26/60 Superdex75 column (GE Healthcare) with gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified and buffer exchanged by size exclusion chromatography using HiLoad 16/600 Superdex-G-200 (GE Healthcare) gel filtration column to storage buffer (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was obtained post the ultracentrifugation at 175,000 g for 1h and loaded onto a HiTrap TALON crude column (GE Healthcare) for affinity enrichment ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...