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Citations for GE Life Sciences :
1301 - 1350 of 1931 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Aurora A protein was thawed and buffer exchanged in the experimental buffer using NAP-5 Columns (GE Healthcare). Experiments typically involved titrating 25 µM of protein in the sample cell with 300 µM of compound in the syringe ...
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bioRxiv - Neuroscience 2023Quote: ... ATXN1 blots were then placed in 5% milk PBST plus 1:2500 rabbit specific HRP antibodies (GE Healthcare) while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated hMDH preps were loaded onto a 5 mL HiTrap Butyl HP column (GE Healthcare). An inverse linear gradient of ammonium sulfate from 1.5 M to 0 M was used to purify hMDH where it eluted at a conductivity of 135 mS/cm ...
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bioRxiv - Biochemistry 2024Quote: ... TEV protease and non-cleaved protein was removed by IMAC (HisTrap FF Crude column, 5 ml, GE Healthcare) with a linear gradient of imidazole (0-500 mM) ...
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bioRxiv - Biochemistry 2024Quote: ... RRM2 (>5 mg mL-1) was injected onto a 24 mL Superdex 75 size exclusion column (GE Healthcare) at a flow rate of 1 mL min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess HaloTag ligand was then removed by purification on desalting NAP-5 or PD-10 columns (GE Healthcare). To generate the HRP-his6Halo-hPKR-N conjugate ...
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bioRxiv - Biochemistry 2024Quote: ... The eluents were pooled and directly loaded onto HiTrap Q HP 5 mL anion exchange column (GE Healthcare) pre-equilibrated with buffer D [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: Time-lapse images were collected at 37°C with 5% CO2 using a DeltaVision ELITE microscope (Applied Precision). We used an apochromatic 100x objective lens (Olympus ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5% glycerol and further enriched with ion exchange Mono S HR 16/10 (SOURCETM 30Q GE Healthcare) to 95% purity ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequently loaded onto a HiTrap SP (CEH-37)/Q (POT proteins) HP 5 mL column (GE Healthcare). An appropriate salt gradient was applied using 20 mM HEPES pH 7.5 buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... passed through a 0.22 μm filter and applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 CV of buffer Q ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was diluted 10-fold and applied to a 5 mL HiTrap Q HP column (GE Healthcare) for ion exchange chromatography using a 50 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: ... The signal of the secondary antibody was detected by using the detect solution mixture (1:1) (ECL™ Prime Western Blotting System, GE Healthcare Life Science) and a luminescent detector (Amersham Imager 600 ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoreactive bands were determined by exposing nitrocellulose blots to a chemiluminescence solution (Denville Scientific Inc., Metuchen, NJ) followed by exposure to Amersham Hyperfilm ECL (GE Healthcare Limited, Buckinghamshire, UK). To determine the relative amounts of protein densitometry Image J version 1.48v (National Institutes of Health ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was collected and incubated for 5 min on 1 mL Ni Sepharose® 6Fast Flow (GE Healthcare) equilibrated with 10 mL binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
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bioRxiv - Biophysics 2021Quote: ... 10 μM ZnCl2 and 1 mM DTT) and loaded into a HiTrap Heparin HP 5 ml column (GE Healthcare) equilibrated with 6.25% of buffer HepB (buffer HepA supplemented with 2 M NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap SP HP 5 ml column (GE Healthcare) for cation exchange ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody dilutions in 5% milk/PBST: anti-MBP HRP conjugated, 1:50,000 (NEB, and anti-GST HRP conjugates, 1:2,000 (GE Healthcare,). Scanned western blot films were quantified using the gel analysis tool in ImageJ.
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bioRxiv - Molecular Biology 2021Quote: ... The protein fraction eluted with 250 mM imidazole was purified further by MonoQ 5/50 GL column (GE Healthcare) at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Filtered supernatant was loaded into a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
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bioRxiv - Neuroscience 2021Quote: ... They were maintained within 37°C incubators at 5% CO2 in DMEM + high glucose (GE Healthcare Bio-Science, SH30022FS) supplemented with 9.09% fetal bovine serum (50 mL added to 500 mL DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Sucrose gradient fractions containing ODA complexes were further purified over a MonoQ 5/50 anion exchange column (GE Healthcare) to separate out other axonemal dynein species ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was then passed over a HisTrap™ FF Ni2+ column (5 ml/min, GE Life Sciences) and washed with Buffer B and the cleaved topo VI collected in the flow through ...
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bioRxiv - Biochemistry 2021Quote: AsLMO10A and AsLPMO10B were purified by anion exchange chromatography using a 5 mL HiTrap DEAE FF column (GE Healthcare) followed by hydrophobic interaction chromatography (HIC ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was additionally purified by ion-exchange chromatography on a “Mono S 5/50 GL” column (GE healthcare) at 0-100% B gradient over 15 column volumes at 0.5 ml/min flow rate using 20 mM sodium phosphate buffer (pH 6.0 at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mM DTT and loaded directly onto a 5 ml HiTrap SP Sepharose fast flow column (GE Healthcare). Bound Nup84-Nup133 was eluted from the column using a 15 column volume (CV ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was diluted to 70 mM NaCl and injected into an HiTrap Heparin HP 5 ml (GE Healthcare) column equilibrated with 20 mM HEPES pH 7.9 ...
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bioRxiv - Biochemistry 2020Quote: Soluble protein was manually applied at room temperature to a 5 mL HiTrapQ anion exchange column (GE Healthcare # 29051325) that had been pre-equilibrated in buffer B ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then further purified by anion exchange chromatography using a 5 ml HiTrap Q HP column (GE Healthcare) across a gradient of 100 mM to 1M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then washed five times for 5 min and proteins were detected with Amersham ECL (GE Healthcare). Images were taken using the iBright Imaging System (Invitrogen).
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bioRxiv - Biophysics 2021Quote: ... The TEV-protease cleaved sample was loaded onto a 5 mL Q-Sepharose HP column (GE Healthcare Life Sciences), equilibrated in low-salt buffer and maintained at ∼4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed cleavage reactions were purified by chromatography on a 5 mL HiTrapQ Sepharose Column (GE Healthcare Life Sciences, USA) (or for SPARTIN ...
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bioRxiv - Immunology 2022Quote: ... 5% Glucose and the proteins purified by cation-exchange chromatography using a HiTrap SP High-Performance columns (GE Healthcare), followed by size exclusion chromatography using a HiLoad 16/600 Superdex 75pg column (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Physiology 2021Quote: ... membranes were incubated for 5 min at room temperature with the Amersham ECL Select peroxidase substrate (RPN2235; GE healthcare), and imaged with the Fusion camera ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare), and after washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was then applied to a HiTrap Q HP 5 mL anion exchange chromatography column (GE Healthcare) attached to an ÄKTA Explorer 100 system (Amersham Pharmacia Biotech) ...