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Citations for GE Life Sciences :
1101 - 1150 of 1931 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The supernatants were initially applied to a HisTrap FF crude 5 mL (GE Healthcare UK Ltd.), which were pre-equilibrated with the HisTrap binding buffer (20 mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was then circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that was inoculated with 100mM CoCl2 for ten minutes ...
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bioRxiv - Biophysics 2023Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... then supernatant was loaded onto a 5 mL Ni2+ affinity column (HisTrap HP, GE Life Sciences) pre-equilibrated with resuspension buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Antibodies were separated from unincorporated fluorophores on desalting columns (NAP-5 or PD-10, GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a 5 ml HiTrap Q HP column (GE Healthcare, Pittsburgh, PA), and the flow-through was collected and dialyzed against 20 mM HEPES pH 6.8 ...
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bioRxiv - Microbiology 2024Quote: ... for 5 min before imaging of the membrane using an Amersham™ Imager 600 (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... The filtered supernatant was loaded onto a HisTrap FF 5 mL (GE Healthcare, Marlborough, MA, USA) pre-equilibrated with 50 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The respective fractions were pooled and applied to a MonoQ 5/50 GL column (GE Healthcare) equilibrated in 50 mM Tris–HCl ...
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bioRxiv - Bioengineering 2024Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer composed of 100 mM tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was loaded on a 5-ml His Trap HP Ni-NTA column (GE Healthcare) equilibrated with lysis buffer A1 ...
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bioRxiv - Biochemistry 2024Quote: ... The complexes were then loaded onto a HiTrap™ Heparin HP 5 ml column (GE Healthcare) and eluted using a NaCl gradient ...
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bioRxiv - Cell Biology 2021Quote: ... A 25 mL aliquot was layered over a 1.073 g·mL−1 Ficoll-Paque™ PLUS solution (GE Healthcare, Chicago, Illinois, United States) and centrifuged at 2000 x g for 25 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were treated with oxygen plasma for 2 min and immediately submerged in Plus-One Repel Silane solution (GE Life Sciences) for 3 min ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 1X Hank’s Balanced Salt Solution (HBSS, SH30588.02, GE Healthcare Life Sciences, US). Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg ...
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bioRxiv - Cell Biology 2022Quote: ... for 2.5 hours at 37 °C and 13mm coverslips were silanized for 3 minutes using ethanol containing 0.37% Bind-Silane solution (GE Healthcare Life Science) and 3.2% acetic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was stained with ethidium bromide solution for 25 minutes followed by visualization on a Typhoon FLA 9500 fluorescence imager (GE Life Sciences) by selecting Cy3 scanning mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated by density gradient centrifugation using the Ficoll® Paque Plus solution (17-1440-02, GE Healthcare).
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved His6-SUMO tag and uncleaved His6-SUMO-GBP2 were separated from tag free GBP2 by passing the solution over a His-Trap column (GE Healthcare/Cytiva). Tag free GBP2 in the flow-through was concentrated and subjected to size exclusion chromatography on a HiLoad16/600 Superdex 200pg column (GE Healthcare/Cytiva ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were injected two times/embryo with mRNAs at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs or plasmid DNA at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... The resulting solutions were then filtered using a 0.22 μm filter unit and applied to a Ni2+-sepharose resin (GE Healthcare, fast flow) pre-equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The gel was stained with Sybr Gold solution for 25 minutes followed by visualization on a Typhoon FLA 9500 fluorescence imager (GE Life Sciences) by selecting Cy3 scanning mode ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 20 minutes then incubated in an excess of blocking solution (1% bovine serum albumin, Sigma-Aldrich, A7906; 1% inactivated horse serum, GE Healthcare B15-023 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were injected with mRNAs at four-cell to eight-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Cell Biology 2020Quote: ... Gels were UV irradiated for 5 minutes and imaged using an Amersham Imager 600 (GE Life Sciences). Gels were then equilibrated in Tris-glycine transfer buffer for 5 minutes and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM β-Mercaptoethanol) with 3C protease and then loaded onto a HisTrap HP column (GE Healthcare). The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The first purification step was affinity chromatography with a 5 mL HisTrap™ FF column (GE Healthcare) precharged with Ni2+ and equilibrated in buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... elution from the HisPur™ Cobalt Resin was applied to a 5 ml streptactin column (GE Healthcare) and washed with HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The filtered sample was applied to a 5 mL HisTrap HP column (GE Healthcare, #17-5248-02), pre-equilibrated with Buffer N and 40 mM imidazole using a peristaltic pump ...
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bioRxiv - Biochemistry 2021Quote: ... The target was captured from the cleared lysate on a 5 ml HisTrap FF column (GE Healthcare) and eluted with a linear gradient from 10 to 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... followed by hydrophobic interaction chromatography (HIC) using a 5 mL HiTrap Phenyl FF (HS) column (GE Healthcare). For the ion exchange procedure ...
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bioRxiv - Biochemistry 2021Quote: AsChi18A was purified by immobilized metal affinity chromatography using a HisTrap FF 5 mL column (GE Healthcare). The periplasmic extract containing AsChi18A was applied on the column using a binding buffer consisting of 20 mM Tris-HCl pH 8.0 and 5 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... The diluent was reloaded on AKTA with 5 mL Hitrap Q HP column (GE Healthcare Life Sciences), followed by a wash with 35 mL buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... combined and further purified by anion-exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare, buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The FdR9-containing flow-through was loaded on a 5 mL HiTrap Q HP column (GE Healthcare) and elution was done using a linear NaCl gradient (0.04 - 1 M ...
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bioRxiv - Biophysics 2020Quote: ... the mixture of tetramers was purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) using a column gradient from 0.1 to 0.4 M NaCl ...
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bioRxiv - Bioengineering 2020Quote: ... The 0.2 μm filtered cleaved product was injected in a 5 mL HiTrap SP HP (GE Healthcare) cationic exchange column and eluted with a gradient to buffer F (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded into a 5 mL Ni–NTA affinity chromatography column (GE Healthcare, United States) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 300 mM NaCl (IMAC B buffer) and loaded onto a 5 mL HiTrap IMAC column (GE Healthcare) using an Äkta Start Chromatography system ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Biophysics 2020Quote: ... The labeled viruses were purified from excess dyes on a Nap-5 gel filtration column (GE Healthcare) that was equilibrated with 50 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a HisTrap column (5 mL, cat. No. 17-5248-01, GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... After cell disruption the cleared lysate was loaded on a 5 ml HisTrap FF column (GE Healthcare) equilibrated in lysis buffer A and the protein was eluted with a gradient of 25 – 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... followed in tandem by a HiTrap™ Q Sepharose HP column (5 ml/min, GE Life Sciences). The SP Sepharose column was removed before the protein was stepped off from the Q sepharose with Buffer B2 (20 mM HEPES pH 7.5 ...